Abstract: Methods and systems for imaging a sample using fluorescence microscopy. High resolution lenses can be used for light sheet microscopy by tilting the excitation beam relative to the imaging optical axis. The excitation beam can be tilted using mirrors, instead of lenses, to generate the tilted illumination sheet. In some examples, a light path starts at a top downward-facing cone of light as it emerges from an optic fiber; a first off-axis parabolic mirror collimates in the x-axis, while a second off-axis parabolic mirror collimates in the y-axis, followed by the photomask to create four primary light sheets, which are then focused by a third off-axis parabolic mirror. As the four primary light sheets converge they create an interference pattern at an objective lens.

Abstract: Methods and systems for imaging a sample using fluorescence microscopy. High resolution lenses can be used for light sheet microscopy by tilting the excitation beam relative to the imaging optical axis. The excitation beam can be tilted using mirrors, instead of lenses, to generate the tilted illumination sheet. In some examples, a light path starts at a top downward-facing cone of light as it emerges from an optic fiber; a first off-axis parabolic mirror collimates in the x-axis, while a second off-axis parabolic mirror collimates in the y-axis, followed by the photomask to create four primary light sheets, which are then focused by a third off-axis parabolic mirror. As the four primary light sheets converge they create an interference pattern at an objective lens.