Abstract: The present disclosure provides nucleic acid constructs for the treatment of cancer, comprising a cancer-specific promoter and one or more therapeutic genes.

Abstract: The presently disclosed subject matter provides a kinetically controlled mixing process, referred to herein as “flash nanocomplexation” or “(FNC),” to accelerate the mixing of a polyanion solution, for example, a plasmid DNA solution, with a polycation solution to match the polyelectrolyte complex (PEC) assembly kinetics through turbulent mixing in a microchamber, thus achieving explicit control of the kinetic conditions for nanoparticle assembly as demonstrated by the tunability of nanoparticle size, composition, hydrodynamic size, hydrodynamic density, surface charge, and polyanion payload.

Abstract: The present disclosure provides nucleic acid constructs for the treatment of cancer, comprising a cancer-specific promoter and one or more therapeutic genes.

Abstract: The present disclosure provides nucleic acid constructs for the treatment of cancer, comprising a cancer-specific promoter and one or more therapeutic genes.

Abstract: A method is disclosed for identifying a member of a peptide library that interacts with a target molecule in situ, the method including expressing immobilised nucleic acid molecules to produce the peptide library in a way that each member of the peptide library is immobilised on the nucleic acid molecule from which it was expressed; contacting the immobilised peptide library with the target molecule; and detecting an interaction between at least one member of the peptide library and the target molecule. The method further comprises sequencing the plurality of nucleic acid molecules in situ on the solid support, such that the at least one member of the peptide library that interacts with the target molecule can be immediately identified, at least by the sequence of the nucleic acid molecule from which it was expressed, without requiring additional or secondary analysis or characterising procedures in order to identify the useful members of the library.

Abstract: A method is provided for isolating protease resistant antimicrobial peptides (AMPs) from a peptide display library. A plurality of nucleic acid constructs that encode displayed peptides are expressed, resulting in the formation of a plurality of peptide-nucleic acid complexes, each complex comprising at least one displayed peptide associated with the corresponding nucleic acid construct encoding the displayed peptide. The complexes are exposed to at least one protease, to allow the proteolysis of protease-sensitive peptides, such that resistant peptides remain. The peptide-nucleic acid complexes are further exposed to a membrane composition to allow association of complexes that contain membrane-associating peptides. Complexes that remain unassociated with the membrane are removed; and membrane-associated complexes are recovered. The AMPs so characterised may be resistant to one or more protease enzymes and exhibit antimicrobial activity against one or more microbe.

Abstract: The invention provides for methods of generating modified polynucleotide libraries by inserting and/or deleting at least three nucleotide residues in polynucleotide sequences. Theses methods may be used with other methods of gene modification such as gene shuffling. The invention further provides methods of directed molecular evolution using the modified polynucleotide libraries produced by these methods.

Abstract: Disclosed herein are methods and compositions for modulating HIV infection by regulating expression of one or more receptors.

Abstract: A method is provided of regulating transcription in a plant cell from a DNA sequence comprising a target DNA operably linked to a coding sequence, which method comprises introducing an engineered zinc finger polypeptide in said plant cell which polypeptide binds to the target DNA and modulates transcription of the coding sequence.

Abstract: By dividing at least the part of the correction optics of at least one row of emitter elements, i.e. the part acting as the fast axis collimator, into several segments, the degradation of beam quality at the focus, especially the widening of the focus, which is caused by the nonconformities between the diode laser bars, or between the row of emitter elements, and the correction optics, can be effectively prevented.

Abstract: We disclose a polypeptide capable of binding to a nucleic acid comprising a receptor nucleotide sequence.

Abstract: A diode laser component has at least one cooling element and at least one laser diode arrangement provided on a mounting surface of the cooling element. The cooling element is produced as a multilayer material or substrate made of several stacked layers joined by their flat sides. These layers consist partially of a metal and partially of an insulating material and/or of a material with a smaller thermal expansion coefficient than metal. At least some of the layers form a cooling plane. At least one cooling channel through which a cooling medium flows is formed in the plane of at least one layer of said cooling plane by at least one recess formed in said layer. Passages or channels are provided for supplying and draining the cooling medium that flows through the cooling channel.