Abstract: A hot foil stamping press with a pressing table (10) on which a sheet can be advanced, a pressing system (20) for pressing a foil (14) onto the sheet, a guiding system (18) for guiding the foil (14) over the sheet, the guiding system (18) defining a foil transportation path which extends across the path along which the sheet is being advanced, a holder (11) for receiving a supply (12) of foil, and a discarding system (22) for the used foil, the holder being arranged at an operator side of the press.

Abstract: Provided are immune cell RNA sequencing methods. In some embodiments, the methods comprise producing a circularized DNA comprising a complementary DNA (cDNA) and a known heterologous sequence, wherein the cDNA is produced from an immune cell RNA. Such methods further comprise performing rolling circle amplification using the circularized DNA as template to produce a concatemer comprising repeating segments comprising the cDNA and the known heterologous sequence. Such methods further comprise sequencing the concatemer or fragments thereof. Also provided are methods comprising producing immune cell RNA sequencing reads using a R2C2 sequencing method, extracting HLA reads from the sequencing reads, and producing allele-specific HLA sequences from the extracted HLA reads. Also provided are computer-readable media, systems, compositions and kits that find use, e.g., in practicing the methods of the present disclosure.

Abstract: A holographic foil supplying device for a hot foil stamping machine has a reel (26) for holographic foil (20), an advancement system (24) for advancing the holographic foil (20) along a movement path (P) in a feeding direction (F) and a frame (22) supporting the reel (26) and the advancement system (24), wherein the frame (22) comprises a mount (30) for mounting the holographic foil supplying device (18) to the hot foil stamping machine. Further, a hot foil stamping machine is shown.

Abstract: A hot foil stamping press with a pressing table (10) on which a sheet can be advanced, a pressing system (20) for pressing a foil (14) onto the sheet, a guiding system (18) for guiding the foil (14) over the sheet, the guiding system (18) defining a foil transportation path which extends across the path along which the sheet is being advanced, a holder (11) for receiving a supply (12) of foil, and a discarding system (22) for the used foil, the holder being arranged at an operator side of the press.

Abstract: Aspects of the present disclosure include methods of producing nucleic acid libraries. In certain aspects, the methods include producing tagged primer extension products, and contacting aliquots of the tagged primer extension products with transposomes to produce tagged extension product fragments. The methods may further include sequencing the tagged extension products and tagged extension product fragments to determine the sequences of nucleic acids of interest. Also provided are compositions and kits that find use, e.g., in practicing embodiments of the methods.

Abstract: A holographic foil supplying device for a hot foil stamping machine has a reel (26) for holographic foil (20), an advancement system (24) for advancing the holographic foil (20) along a movement path (P) in a feeding direction (F) and a frame (22) supporting the reel (26) and the advancement system (24), wherein the frame (22) comprises a mount (30) for mounting the holographic foil supplying device (18) to the hot foil stamping machine. Further, a hot foil stamping machine is shown.

Abstract: Provided are methods of preparing high molecular weight nucleic acids for analysis. In certain embodiments, the methods comprise migrating nucleic acids comprising high molecular weight nucleic acids through a polymeric matrix, excising a portion of the polymeric matrix comprising high molecular weight nucleic acids, and isolating the high molecular weight nucleic acids from the excised polymeric matrix. The isolating comprises immobilizing the high molecular weight nucleic acids on particulate solid supports, and eluting the high molecular weight nucleic acids from the particulate solid supports. The methods may further comprise analyzing the isolated high molecular weight nucleic acids. According to some embodiments, the analyzing comprises sequence analysis of the isolated high molecular weight nucleic acids, e.g., using a nanopore- or zero mode waveguide (ZMW)-based sequencing device.

Abstract: Provided are methods of nucleic acid sequencing. The methods include producing a circularized DNA including a full-length cDNA and a known heterologous sequence, and performing rolling circle amplification using the circularized DNA as template to produce a concatemer including repeating segments including the full-length cDNA and the known heterologous sequence. The methods further include obtaining a raw sequencing read of the concatemer using a nanopore, identifying the repeating segments in the raw sequencing read, and producing a consensus sequence of the full-length cDNA based on the sequences of the repeating segments. Computer-readable media, computing devices, and systems that find use, e.g., in practicing the methods of the present disclosure are also provided.

Abstract: Aspects of the present disclosure include methods of sequencing antibody chains from hybridomas. In some embodiments, the methods comprise contacting hybridoma RNA with a constant region-specific reverse transcription primer, and contacting the hybridoma RNA with a reverse transcriptase with terminal nucleotidyl transferase activity, thereby creating a heavy chain and/or light chain cDNA. Such methods further comprise contacting the cDNA with a template switch oligonucleotide comprising a universal PCR primer sequence, thereby generating a single stranded cDNA, and performing second strand synthesis on the single stranded cDNA, thereby producing a double stranded heavy chain and/or light chain cDNA. A heavy chain and/or light chain sequencing template is produced, and the methods further comprise sequencing the template by Sanger sequencing. Also provided are kits that find use, e.g., in practicing the methods of the present disclosure.

Abstract: Aspects of the present disclosure include methods of producing nucleic acid libraries. In certain aspects, the methods include producing tagged primer extension products, and contacting aliquots of the tagged primer extension products with transposomes to produce tagged extension product fragments. The methods may further include sequencing the tagged extension products and tagged extension product fragments to determine the sequences of nucleic acids of interest. Also provided are compositions and kits that find use, e.g., in practicing embodiments of the methods.

Abstract: Aspects of the present disclosure include methods of producing nucleic acid libraries. In certain aspects, the methods include producing tagged primer extension products, and contacting aliquots of the tagged primer extension products with transposomes to produce tagged extension product fragments. The methods may further include sequencing the tagged extension products and tagged extension product fragments to determine the sequences of nucleic acids of interest. Also provided are compositions and kits that find use, e.g., in practicing embodiments of the methods.

Abstract: Aspects of the present disclosure include methods of producing nucleic acid libraries. In certain aspects, the methods include producing tagged primer extension products, and contacting aliquots of the tagged primer extension products with transposomes to produce tagged extension product fragments. The methods may further include sequencing the tagged extension products and tagged extension product fragments to determine the sequences of nucleic acids of interest. Also provided are compositions and kits that find use, e.g., in practicing embodiments of the methods.

Abstract: A muffler for an exhaust system of an internal combustion engine having a housing wall, having a muffler housing with at least one inlet pipe guided through the housing wall and at least one outlet pipe guided through the housing wall, wherein the inlet pipe has at least one outflow opening via which the exhaust gas can flow from the inlet pipe into the outlet pipe, whereby, within the muffler housing, at least one coupling chamber with a chamber wall is provided with a center axis, which forms a flow channel between the inlet pipe and the outlet, whereby the chamber wall encloses the inlet pipe or the outlet pipe in the receiving direction E1 with respect to the center axis and is connected only indirectly via the inlet pipe and the outlet pipe or a muffler housing intermediate wall in a form- or material-fitting manner to the housing wall, whereby the chamber wall of the coupling chamber has a diameter D and whereby the exhaust pipe has an outer diameter d, whereby for the ratio of D/d, 20/10>?D/d>=11

Abstract: A muffler for an exhaust system of an internal combustion engine having a housing wall, having a muffler housing with at least one inlet pipe guided through the housing wall and at least one outlet pipe guided through the housing wall, wherein the inlet pipe has at least one outflow opening via which the exhaust gas can flow from the inlet pipe into the outlet pipe, whereby, within the muffler housing, at least one coupling chamber with a chamber wall is provided with a centre axis, which forms a flow channel between the inlet pipe and the outlet, whereby the chamber wall encloses the inlet pipe or the outlet pipe in the receiving direction E1 with respect to the centre axis and is connected only indirectly via the inlet pipe and the outlet pipe or a muffler housing intermediate wall in a form- or material-fitting manner to the housing wall, whereby the chamber wall of the coupling chamber has a diameter D and whereby the exhaust pipe has an outer diameter d, whereby for the ratio of D/d, 20/10>?D/d>=11