Abstract: A base for use with scaffolding. The base includes side support members and cross members. The side support members have scaffold receiving members provided at ends thereof. The cross members extend between the side support members and are rotatably mounted to the side support members. Wheels are mounted to the cross members. As the cross members are rotated from a first position to a second position, the wheels are moved from an initial position in which the wheels are spaced from a ground or floor surface to a second position in which the wheels are positioned in engagement with the ground or floor surface.

Abstract: A system including a gearbox and a hydraulic machine connected by a working fluid circuit. The gearbox including a gearbox housing having first and second gearbox fluid passages integrated into the gearbox housing. The hydraulic machine including a case having first and second hydraulic machine fluid passages, the first and second hydraulic machine fluid passages being integrated into the case. A set of rotating elements enclosed within the case. The first gearbox fluid passage and the first hydraulic machine fluid passage align to form a feed passage to conduct fluid at a first pressure from a first source to the set of rotating elements. The second gearbox fluid passage and the second hydraulic machine fluid passage align to form a charge passage to conduct fluid at a second pressure from a second source to the set of rotating elements. The first pressure being less than the second pressure.

Abstract: Muconic acid is a molecule that can be converted into direct replacement chemicals for incumbent petrochemicals and performance-advantaged bioproducts. Disclosed herein are Pseudomonas putida KT2440 that are engineered to convert glucose and xylose, the primary carbohydrates in lignocellulosic hydrolysates, to muconic acid using a model-guided strategy to maximize the theoretical yield. Using adaptive laboratory evolution (ALE) and metabolic engineering in a strain engineered to express the D-xylose isomerase pathway, we demonstrated that mutations in the heterologous D-xylose:H+ symporter (XylE), increased expression of a major facilitator superfamily transporter (PP_2569), and overexpression of aroB encoding the native 3-dehydroquinate synthase, enable efficient muconic acid production from glucose and xylose simultaneously. Using the rationally engineered strain, we produced 33.7 g/L muconate at 0.18 g/L/h and a 46% molar yield (92% of the maximum theoretical yield).

Abstract: Disclosed herein are methods and compositions that enable the production of branched-chain polyhydroxyalkanoates, branched-chain 3-hydroxyacids (BCHA) (PHA monomers), and branched-chain fatty acids (BCFA) in Pseudomonas putida KT2440. The branched-chain molecules enabled by this platform enable novel chemistries that are not accessible via the existing paradigm which is limited to straight-chain molecules. Further disclosed herein are methods and compositions for deciphering the mechanisms of bacterial fatty acid biosynthesis is crucial for both the engineering of bacterial hosts to produce fatty acid-derived molecules and the development of new antibiotics.

Abstract: The present disclosure relates to a genetically modified microbial cell that includes a first genetic modification resulting in the expression of an exogenous vanillate demethylase, such that the microbial cell is capable of metabolizing an S-lignin decomposition product and producing 2-pyrone-4,6-dicarboxylate (PDC).

Abstract: Disclosed herein are the genetically modified Pseudomonas with improved tolerance to hydroxycinnamic acids.

Abstract: Disclosed herein are compositions and methods to improve Cupriavidus sp. as a host for formate conversion. Also disclosed herein are compositions and methods to improve growth of non-naturally occurring Cupriavidus sp. on carbon dioxide, succinate, formate and fructose as sole carbon sources.

Abstract: The present disclosure relates to a non-naturally occurring microorganism that includes an endogenous genetic deletion that eliminates the expression of at least a pyruvate kinase, where the genetically modified prokaryotic microorganism is capable of producing 3-deoxy-D-arabino-heptulosonate-7-phosphate.

Abstract: Disclosed herein are engineered Pseudomonas useful to relieve the metabolic bottleneck of 4-hydroxybenzoate transformation in a muconate accumulating strain on an engineered Pseudomonas by swapping its endogenous para-hydroxybenzoate-3-hydroxylase (PHBH), PobA, with a homolog, PraI, that has a broader cofactor preference.

Abstract: An aspect of the present disclosure is a microbial cell that includes a genetic modification resulting in the expression of a deficient form of an endogenous dioxygenase, and a gene encoding an exogenous dioxygenase and a promoter sequence, where the endogenous dioxygenase includes PcaH and PcaG, the exogenous dioxygenase includes LigA and LigB, the microbial cell is capable of growth utilizing at least one of a cellulose decomposition molecule or a lignin decomposition molecule, and the microbial cell is capable of producing 2-hydroxy-2H-pyran-4,6-dicarboxylic acid.

Abstract: The present disclosure relates to a genetically modified microbial cell that includes a first genetic modification resulting in the expression of an exogenous vanillate demethylase, such that the microbial cell is capable of metabolizing an S-lignin decomposition product and producing 2-pyrone-4,6-dicarboxylate (PDC).

Abstract: The present disclosure relates to a non-naturally occurring microorganism that includes a gene encoding a MucK transporter protein, where the microorganism is capable of catabolizing terephthalic acid (TPA). In some embodiments of the present disclosure, the gene encoding the MucK transporter protein may contain at least one mutation, relative to a reference gene encoding a reference MucK transporter protein.

Abstract: Disclosed herein are compositions of non-naturally occurring enzymes to enable microbial syringol utilization with GcoAB.

Abstract: The present disclosure relates to a genetically modified microbial cell that includes a genetic modification resulting in the expression of a vanillate demethylase, where the microbial cell is capable of metabolizing at least one S-lignin decomposition molecule including at least one of syringate and/or 3-O-methyl gallate, and the genetically modified microbial cell is capable of producing gallate. In some embodiments of the present disclosure, the vanillate demethylase may include VanAB.

Abstract: Disclosed herein are methods and compositions for catalytic glycolysis to deconstruct PET to bis(2-hydroxyethyl) terephthalate (BHET). For BHET conversion to terephthalate and ethylene glycol, we engineer Pseudomonas putida KT2440 with PETase and MHETase enzymes from Ideonella sakaiensis. We further engineer P. putida to convert terephthalate to a performance-advantaged bioproduct, ?-ketoadipic acid, and for improved utilization of ethylene glycol, a byproduct of BHET catabolism. In a bioreactor, we produce 15.1±0.6 g/L of ?-ketoadipic acid (?KA) from BHET at 76±3% molar yield. Lastly, we demonstrate conversion of catalytically depolymerized PET to ?KA. Overall, this work highlights the potential of tandem catalytic deconstruction and biological conversion as a means to upcycle waste PET.

Abstract: Disclosed herein are the genetically modified Pseudomonas with improved tolerance to hydroxycinnamic acids.

Abstract: Disclosed herein are engineered P. putida KT2440 co-expressing PETase and MHETase enzymes that selectively degrades PET into monomers, ethylene glycol and terephthalate (TPA). In another embodiment, disclosed herein are methods for making and using a highly efficient EG metabolizing P. putida KT2440 strain. Given that native P. putida does not have a TPA metabolic pathway, nor the proteins to transport TPA into the cell, the next metabolic engineering challenge for developing synthetic P. putida strain to plastic upcycling was enabling TPA catabolism in P. putida KT2440. TPA transporters and catabolic pathway have been characterized in several microorganisms including Comamonas sp. strain E6 and Rhodococcus jostii RHA1.

Abstract: An apparatus and method for creating the apparatus that holds a bolt open after a last round is fired from a magazine is disclosed. A lower receiver may include at least two cavities. A transfer bar may be configured to fit into a first cavity of the at least two cavities. A hold down may be configured to fit into a second cavity of the at least two cavities. At least a portion of the hold down may be configured to overlap with at least a portion of the transfer bar to hold the transfer bar in place. The transfer bar, when a final round is fired from a magazine in the lower receiver, may be pushed up by a bump in the magazine to transfer upward movement to the transfer bar that catches a bolt and holds the bolt open until activation of a release button.

Abstract: A method and apparatus for enabling handgun magazines to function in a rifle lower forging, may include a rifle lower forging. The rifle lower forging may include but is not limited to a recessed area on a side portion of the rifle lower forging configured to receive a handgun magazine release. The rifle lower forging may further include a vertical hole on a top portion of the rifle lower forging configured to receive an elongated portion to hold the handgun magazine release. The rifle lower forging may further include a hole perpendicular to the vertical hole configured to receive a handgun magazine release spring. The rifle lower forging may further include a horizontal slit on the top portion of the rifle lower forging configured to receive an ejector. The rifle lower forging may further include one or more apertures perpendicular to the horizontal slit each configured to receive a fastener to secure the ejector to the rifle lower forging.

Abstract: Disclosed herein are enzymes and organisms useful for the dealkylation of products derived from lignin depolymerization, including the conversion of guaiacol or guaethol to catechol or the conversion of anisole to phenol. Methods of converting guaiacol or guaethol to catechol or anisole to phenol using enzymes or organisms expressing the same are also disclosed.