Abstract: The present application discloses two anti-GDF15 neutralizing monoclonal antibodies and uses thereof. The variable regions of the light chain and heavy chain of the antibodies G1 and G2 with neutralizing activity are provided. Anti-GDF15 mouse monoclonal antibodies were prepared by the hybridoma technology, anti-GDF15 hybridoma cell lines capable of stable secretion with high specificity were screened out, ascites were prepared to obtain high-specificity and high-affinity anti-GDF15 monoclonal antibodies and two anti-GDF15 monoclonal antibodies with neutralizing activity were identified for the first time by constructing an antibody neutralization detection system.