Abstract: A recombinant vector, transgenic fish egg using the same and biomaterial using the same are applied to provide a transgenic fish that secreting recombinant human procollagens or collagens, and further to provide the biomaterial having the recombinant human procollagens or collagens and extract the recombinant human procollagens or collagens from the part(s), having the recombinant human procollagens or collagens, of the transgenic fish.

Abstract: The present invention discloses a cell culture realtime observation system comprising a plate, a culture well, an image capture device, at least one container, an evaporation device, a first micro channel, a second micro channel and a transmission unit. The culture well is disposed on the plate, and the image capture device is disposed between the plate and the culture well. The container is disposed on the plate and at a side of the culture well, and the evaporation device is disposed at the other side of the culture well. The container and the culture well are connected by the first micro channel, and the culture well and the evaporation device are connected by the second micro channel. The transmission unit is electrically connected to the image capture device.