Abstract: The present disclosure provides methods and systems for systematic elimination of background DNA in a DNA mixture sample. Often, the DNA of interest is in a heavy background of DNAs from other tissues. For example, a majority of DNA in plasma cell-free DNA originates from white blood cells. The present disclosure exploits genome-wide background DNA methylation to systematically eliminate DNA from white blood cells and normal tissue(s), therefore enriching non-background DNA for diagnostics, for example, the diagnostics of cancer and infectious diseases. The methods and systems may comprise the selection of targeted regions to generate one or more hybrid capture panels, digest nucleic acid molecules of specific methylation status with one or more restriction enzymes, retrieve the remaining DNA using the hybrid capture panel, sequence the captured DNA, analyze the sequencing data of the captured DNA, and diagnose diseases.

Abstract: The present disclosure provides methods and systems for detecting or inferring levels of Copy Number Variants (CNVs) in cell-free nucleic acid samples to detect or assess cancer and prenatal diseases. Cell-free nucleic acid methylation sequencing data may be utilized to distinguish tumor-derived or fetal-derived sequencing reads from normal cfDNA sequencing reads. Each cell-free nucleic acid sequencing read (e.g., containing tumor or fetal methylation markers) may be classified as corresponding to a tumor/fetal-derived or a normal-plasma cell-free nucleic acid, based on the methylation cfDNA sequencing data (e.g., obtained using Bisulfite sequencing or bisulfite-free sequencing methods) and tumor/fetal methylation markers. Next, a profile of the tumor/fetal-derived sequencing read counts may be constructed and then normalized. The CNV status (e.g., gain or loss) of each genomic region may be inferred, and a diagnosis or prognosis can be made based on a subjects inferred CNV profile.

Abstract: The subject of the present invention is a valve for extracting, by pumping, a fluid contained in a space, said valve comprising: a) a main body having a first opening for the passage of said fluid during pumping, and b) an indicator means configured to indicate whether or not the fluid is passing through the first opening.

Abstract: The subject of the present invention is a valve for extracting, by pumping, a fluid contained in a space, said valve comprising: a) a main body having a first opening for the passage of said fluid during pumping, and b) an indicator means configured to indicate whether or not the fluid is passing through the first opening.

Abstract: The present invention discloses a backing plate for storage bags as well as a storage bag and a storage box with the same. With this backing plate including a backing plate body, a trapezoid or triangular edge fold is formed as an extension to one end of the backing plate body, and another trapezoid or triangular edge fold as an extension to the other end of the backing plate body. With the backing plate arranged in the storage bag and the storage box, there are no requirements for the two edge folds extended outwards at the bottom of the storage bag. This will not only strengthen the stereoscopic perception of the storage bag, but also save materials and reduce the production cost, better satisfying the users' requirements.

Abstract: The present invention relates to an environmental protection double layer oil smoke filter device for kitchen range, in which the main body is primarily assembled from a working area, a placement area and a pump water drainage system, while the oil smoke filtering device is assembled from an electrostatic processing device, a draft device and a ventilating device. Accordingly, a pump water drainage system connected to a liquid containing groove located at the bottom portion of the filtering device is used to effectively achieve the facility to separate oil and water within the liquid containing groove, moreover, the separated water can be reused.