Abstract: Albumin and doxorubicin are bonded through reaction. The bonded albumin and doxorubicin are added with a gold-nanoparticles solution for reaction. After being purified with water in a column, the liquid phase of gold-nanoparticles is changed into a phosphate buffer for obtaining an anticancer drug having doxorubicin bonded with gold nanoparticles. Thus, with the high biocompatibility and the big surface areas, gold nanoparticles are used in biological applications and used as carriers for increasing drug accumulation on tumor. The bonding between gold nanoparticles and doxorubicin increases stability of doxorubicin. Besides, doxorubicin has tumor-killing effect for cancer therapy.

Abstract: A bonding method is provided for gold nanoparticles (GNPs). GNPs are bonded with diethylenetriamine pentaacetic acid (DTPA). GNPs have high bio-compatibility and high surface area. Hence, the present invention uses GNPs as carriers for diagnosing and treating cancer.

Abstract: A bonding method is provided for gold nanoparticles (GNPs). GNPs are bonded with diethylenetriamine pentaacetic acid (DTPA). GNPs have high bio-compatibility and high surface area. Hence, the present invention uses GNPs as carriers for diagnosing and treating cancer.

Abstract: The present disclosure describes techniques used for colorectal cancer detection. Position and distribution of colorectal cancer tumor are detected through nuclear imaging. Alternatively, stage of colorectal cancer is identified by shading value in tumor. Thus, the present disclosure provides a safe and noninvasive clinical method for diagnosing and tracing level and distribution of colorectal cancer before and after treatment.

Abstract: Human neutrophil peptide (HNP) 1-3, used as diagnostic and therapeutic molecular probes, are found in clinical tissues of gastric cancer patients. In the analytical process according to the present disclosure, pairs of gastric cancer tissues are used to seek the putative biomarkers by proteomic strategy based on matrix assisted laser desorption ionization-imaging mass spectrometry (MALDI-IMS). Then, three differential biomarkers, including HNP-1, -2 and -3, are identified (P<0.001) and overexpressed in gastric cancer. At last, western blotting and immunohistochemistry are used to validate the protein expression in gastric cancer tissues. In conclusion, the use of the up-regulated proteins, HNP1-3, helps diagnosis and therapy in clinical for gastric cancer after validating the sensitivity and specificity.

Abstract: A method for diagnosing liver fibrosis is provided. A monoclonal antibody and an antagonist of cannabinoid receptor in liver fibrosis cell are labeled. The monoclonal antibody or the antagonist is injected for nuclear imaging. Thus, through the image obtained through the nuclear imaging, liver fibrosis is diagnosed and traced in clinic use for preventive medicine.

Abstract: The present disclosure describes techniques used for colorectal cancer detection. Position and distribution of colorectal cancer tumor are detected through nuclear imaging. Alternatively, stage of colorectal cancer is identified by shading value in tumor. Thus, the present disclosure provides a safe and noninvasive clinical method for diagnosing and tracing level and distribution of colorectal cancer before and after treatment.

Abstract: Human neutrophil peptide (HNP) 1-3, used as diagnostic and therapeutic molecular probes, are found in clinical tissues of gastric cancer patients. In the analytical process according to the present disclosure, pairs of gastric cancer tissues are used to seek the putative biomarkers by proteomic strategy based on matrix assisted laser desorption ionization-imaging mass spectrometry (MALDI-IMS). Then, three differential biomarkers, including HNP-1, -2 and -3, are identified (P<0.001) and overexpressed in gastric cancer. At last, western blotting and immunohistochemistry are used to validate the protein expression in gastric cancer tissues. In conclusion, the use of the up-regulated proteins, HNP1-3, helps diagnosis and therapy in clinical for gastric cancer after validating the sensitivity and specificity.

Abstract: Protein biomarkers are selected for diagnosing Alzheimer's disease. Samples of Alzheimer's disease are used to find the biomarkers. It is done through methods including 2-dimentional differential in-gel electrophoresis (2D-DIGE), isotope-coded protein labeling (ICPL) and western blotting. Through examining density differences of the selected biomarkers, Alzheimer's disease can be early diagnosed or prevented.

Abstract: Disclosed are serum biomarkers for diagnosing liver fibrosis and methods for measuring the same. The serum biomarkers obtained from human serum include alpha2-macroglobulin (“A2M”), vitamin D binding protein (“VDBP”), apolipoprotein AI (“ApoAI”). The methods involve with immunoassay using specific antibodies to detect the biomarkers, including enzyme-linked immunosorbent assay (“ELISA”), radio immune assay (“RIA”) and flexible multi-analyte profiling (“xMAP”). ELISA, RIA or xMAP is used to measure changes of protein concentration for the specific protein biomarkers in serum for diagnosing liver fibrosis with suffering hepatitis B or C or other liver diseases. The measurement is safe and accurate. The method can be used before and after treatment of liver fibrosis. Thus, it is possible to achieve early diagnosis and treatment advocated in the preventive medicine.