Abstract: Disclosed is a marking method for marking a radiation therapy positioning note, comprising in sequence: a preparing step of providing a radiation therapy positioning sticker, wherein the radiation therapy positioning sticker includes a pattern layer and a release layer disposed on an outer surface of the pattern layer, and the radiation therapy positioning note is formed on the outer surface of the pattern layer; a transferring step of attaching the radiation therapy positioning sticker onto a predetermined note position of a radiation therapy target object in a manner that an inner surface of the pattern layer faces forward the predetermined note position and covers the predetermined note position; and a removing step of peeling off the release layer from the outer surface of the pattern layer to outwardly expose the radiation therapy positioning note, which is formed on the outer surface of the pattern layer covering the predetermined note position.