Abstract: The present invention relates to a method for effectively proliferating regulatory T cells, by which, particularly, in the presence of a fusion protein dimer comprising IL-2. protein or a variant thereof and CD80 protein or a fragment thereof, CD4+, CD25+, and CD127? T cells can be effectively proliferated. In particular, when combined with a predetermined cell culture medium, regulatory T cells such as CD4+, CD25+, and CD127? can be effectively and specifically proliferated. In addition, when the method is used, it has been confirmed that the survival rate of regulatory T cells is remarkably increased as compared to a conventionally used culture method using IL-2, and a significant increase in the yield of Foxp3+ regulatory T cells has been confirmed. Thus, such a proliferation method can be used in the field of cell therapeutic agents using regulatory T cells.

Abstract: The present invention relates to a fusion protein including an IL15 protein, an IL15 receptor alpha (IL15R?) protein, an Fc domain, and an IL2 protein, and a use thereof, and more specifically, to: a fusion protein including an IL15 protein, an IL15R? protein, an Fc domain, and an IL2 protein; a dimer including the fusion protein; a composition that is for culturing natural killer (NK) cells and includes the fusion protein or dimer; and a method for culturing NK cells. The fusion protein according to the present invention makes it possible to efficiently proliferate and culture NK cells while maintaining the cytolytic activity of the NK cells and promoting the activation of the NK cells when culturing the NK cells, thus making it possible to obtain a large number of NK cells without using feeder cells. Therefore, the fusion protein is useful for the commercialization of NK cells into safe cell therapy agents.

Abstract: The present invention relates to a method for effectively proliferating regulatory T cells, by which, particularly, in the presence of a fusion protein dimer comprising IL-2 protein or a variant thereof and CD80 protein or a fragment thereof, CD4+, CD25+, and CD127? T cells can be effectively proliferated. In particular, when combined with a predetermined cell culture medium, regulatory T cells such as CD4+, CD25+, and CD127? can be effectively and specifically proliferated. In addition, when the method is used, it has been confirmed that the survival rate of regulatory T cells is remarkably increased as compared to a conventionally used culture method using IL-2, and a significant increase in the yield of Foxp3+ regulatory T cells has been confirmed. Thus, such a proliferation method can be used in the field of cell therapeutic agents using regulatory T cells.

Abstract: Provided is an anticancer agent, comprising, as active ingredients, NK cells and a fusion protein which comprises an IL-2 protein and CD80 protein. In one specific embodiment, a fusion protein comprising a CD80 fragment, an immunoglobulin Fc and an IL-2 variant can activate immunocytes such as natural killer cells. In addition, since cancer can be effectively inhibited when co-administering with natural killer cells, the pharmaceutical composition increases the immune activity in the body so as to be effectively usable for cancer, there by having high industrial applicability.

Abstract: The present invention relates to a composition for proliferating a T cells, containing a fusion protein dimer comprising IL-2 protein or a variant thereof and CD80 protein or a fragment thereof, and to a method for culturing T cells using same. The T cells cultured according to the present invention increase the proliferation and activity of T cells even without using CD3/CD28 antibody-bound magnetic beads and proliferate T cells by culturing a patient's own peripheral blood mononuclear cells and are not likely to cause side effects in the human body, and thus will be widely used as a novel T cell therapeutic agent. Furthermore, in the case of CD8+ T cells cultured as described above, the activity thereof increases, and thus, the CD8+ T cells can be used as a more effective therapeutic agent.

Abstract: The invention relates to: a composition for culturing natural killer cells, comprising, as an active ingredient, a fusion protein comprising an IL-2 protein and a CD80 protein; and a method for preparing natural killer cells by using same. Particularly, a composition for culturing natural killer cells, comprising, as an active ingredient, a fusion protein comprising IL-2 or a variant thereof and CD80 or a fragment thereof, of the present invention; promotes the proliferation of natural killer cells, induces the expression of CD16 and NKp46, and increases the expression and secretion of granzyme B and perforin, thereby being effectively usable in the preparation of natural killer cells having an excellent anticancer immune function.

Abstract: The present invention relates to a method for effectively proliferating regulatory T cells, by which, particularly, in the presence of a fusion protein dimer comprising IL-2 protein or a variant thereof and CD80 protein or a fragment thereof, CD4+, CD25+, and CD127? T cells can be effectively proliferated. In particular, when combined with a predetermined cell culture medium, regulatory T cells such as CD4+, CD25+, and CD127? can be effectively and specifically proliferated. In addition, when the method is used, it has been confirmed that the survival rate of regulatory T cells is remarkably increased as compared to a conventionally used culture method using IL-2, and a significant increase in the yield of Foxp3+ regulatory T cells has been confirmed. Thus, such a proliferation method can be used in the field of cell therapeutic agents using regulatory ‘I’ cells.

Abstract: Disclosed herein is an arithmetic logic unit over a finite field GF(2m). Arithmetic logic units consistent with the present invention are disclosed as implemented using a division algorithm based on a binary greatest common divisor algorithm and a Most Significant Bit-first multiplication algorithm. The arithmetic logic unit can perform both a multiplication and a division using shared logic. Since the arithmetic logic unit has no limitations in the selection of an irreducible polynomial, and it is very regular and easily formed as a module, the arithmetic logic unit of the present invention has high expansibility and flexibility with respect to the size m of a field. Further, since the arithmetic logic unit of the present invention can perform a multiplication and a division using shared logic, it is very suitable to implement an encryption system for application products requiring a small size, such as smart cards or wireless communication devices.

Abstract: Provided is an apparatus for continuously manufacturing a plurality of high-quality billets containing fine, uniform spherical particles, with improvements in energy efficiency and mechanical properties, cost reduction, convenience of casting, and shorter manufacturing time.

Abstract: Provided is a rheoforming apparatus that ensures the manufacture of products with fine, uniform spherical particles, with improvements in energy efficiency and mechanical properties of the products, cost reduction, convenience of forming, and shorter manufacturing time. The apparatus includes a first sleeve, an end of which is formed with an outlet vent for releasing slurries; a second sleeve for receiving molten metals, an end of the second sleeve being hinge-connected to the other end of the first sleeve at a predetermined angle; a stirring unit for applying an electromagnetic field to an area of the second sleeve in which the molten metals are present; a plunger, which is inserted into the other end of the second sleeve to block the other end of the second sleeve for receiving the molten metals and to pressurize the slurries; and a forming unit, which is connected to the outlet vent of the first sleeve to form products with a predetermined shape using the slurries.

Abstract: Provided is an apparatus for manufacturing a high-quality semi-solid metallic slurry containing fine, uniform spherical particles that can be readily and conveniently applied to a subsequent process, with improvements in energy efficiency and mechanical properties, cost reduction, convenience of casting, shorter manufacturing time, and easy discharge. The apparatus includes at least one sleeve for receiving molten metals in liquid state; a stirring unit for applying an electromagnetic field to the molten metals in the sleeve; at least one plunger for defining the bottom of a space where the molten metals are loaded, the plunger being inserted into an end of the sleeve; and a driving unit for driving the plunger upward and downward.

Abstract: Provided is a rheoforming apparatus that ensures the manufacture of products with fine, uniform spherical particles, with improvements in energy efficiency and mechanical properties of the products, cost reduction, convenience of forming, and shorter manufacturing time. The apparatus includes a first sleeve, an end of which is formed with an outlet vent for releasing slurries; a second sleeve for receiving molten metals, an end of the second sleeve being hinge-connected to the other end of the first sleeve at a predetermined angle; a stirring unit for applying an electromagnetic field to an area of the second sleeve in which the molten metals are present; a plunger, which is inserted into the other end of the second sleeve to block the other end of the second sleeve for receiving the molten metals and to pressurize the slurries; and a forming unit, which is connected to the outlet vent of the first sleeve to form products with a predetermined shape using the slurries.

Abstract: Provided is an apparatus for continuously manufacturing a plurality of high-quality billets containing fine, uniform spherical particles, with improvements in energy efficiency and mechanical properties, cost reduction, convenience of casting, and shorter manufacturing time.

Abstract: Disclosed herein is an arithmetic logic unit over a finite field GF(2m). Arithmetic logic units consistent with the present invention are disclosed as implemented using a division algorithm based on a binary greatest common divisor algorithm and a Most Significant Bit-first multiplication algorithm. The arithmetic logic unit can perform both a multiplication and a division using shared logic. Since the arithmetic logic unit has no limitations in the selection of an irreducible polynomial, and it is very regular and easily formed as a module, the arithmetic logic unit of the present invention has high expansibility and flexibility with respect to the size m of a field. Further, since the arithmetic logic unit of the present invention can perform a multiplication and a division using shared logic, it is very suitable to implement an encryption system for application products requiring a small size, such as smart cards or wireless communication devices.

Abstract: Provided are a method and apparatus for continuously manufacturing quality billets for thixocasting that have a fine, uniform, spherical particle structure, in a short time, with improvements in energy efficiency and mechanical properties, cost reduction, convenience of casting, and shorter manufacturing time. The method involves applying an electric field to a domain of a sleeve defined by first and second plungers inserted through each end of the sleeve and loading a molten metal into the domain of the sleeve to form a semi-solid metallic slurry, moving the first plunger toward the second plunger to compress the semi-solid metallic slurry and form a billet via cooling, and shifting the billet toward the second plunger to discharge the billet from the sleeve.

Abstract: The present invention provides a method and apparatus for manufacturing a high-quality semi-solid metallic slurry containing fine, uniform spherical particles that can be readily and conveniently applied to a subsequent process, with improvements in energy efficiency and mechanical properties, cost reduction, convenience of casting, and shorter manufacturing time. The semi-solid metallic slurry manufacturing method includes applying an electromagnetic field to a space containing a slurry vessel, loading a molten metal into the slurry vessel in a state where the electromagnetic field is applied to the space, and drawing the slurry vessel out from the space.

Abstract: A method for manufacturing a metallic material for rheocasting or thixoforming and a metallic material formed using the method are provided. The method includes: applying an electromagnetic field to a vessel and loading a molten metal into the vessel; and cooling the molten metal to form a metallic material for rheocasting or thixoforming. The entire volume of molten metal is rapidly and uniformly cooled throughout, from the wall toward the center of the vessel, without generating latent heat caused by the formation of solidification layers at the early stage of cooling. The molten metal in the vessel is cooled rapidly below its liquidus temperature within 1-10 seconds after the loading of the molten metal into the vessel, so that numerous uniform crystal nuclei are created throughout the entire volume of molten metal to form a metallic material having uniform, micro, spherical particles.