Abstract: Disclosed is a method comprising extracting a gene sequence of a first gene from a first database in response to receiving a user's input, generating segmented sequences on the basis of the reverse complementary sequence of the gene sequence of the first gene, identifying, based on comparison of the segmented sequences with gene sequences of a second database, at least one matched sequence corresponding to at least one segmented sequence of the segmented sequences.

Abstract: The present invention relates to an anti-tumor adenovirus and an anticancer composition comprising same. Double-stranded siRNA of the present invention simultaneously inhibits the expression of a first nucleic acid and a second nucleic acid, thus promoting the death of cancer cells, exhibits more remarkable anticancer activity as compared to co-treatment of respective siRNAs, has a synergistic effect of improving cancer cell death in combined treatment with an anticancer agent. The adenovirus comprising a shRNA-encoding expression cassette expressing the double-stranded siRNA, and a hTERT promoter evades immune responses in the body and is specifically delivered to cancer cells, thus having a systemic therapeutic effect, can be locally delivered, has excellent selectivity, and exhibits a significant anticancer effect even in minimally invasive treatment, and thus, the adenovirus can be effectively used as an anticancer composition or an anticancer adjuvant in various carcinomas.