Patents by Inventor Chung-Jr HUANG

Chung-Jr HUANG has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220364137
    Abstract: The present invention relates to methods of upregulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture by manipulating the mannose to total hexose ratio in the cell culture media formulation.
    Type: Application
    Filed: July 18, 2022
    Publication date: November 17, 2022
    Applicant: Amgen Inc.
    Inventors: Chung-Jr HUANG, Xiaoming YANG
  • Patent number: 11434514
    Abstract: The present invention relates to methods of upregulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture by manipulating the mannose to total hexose ratio in the cell culture media formulation.
    Type: Grant
    Filed: November 22, 2021
    Date of Patent: September 6, 2022
    Assignee: Amgen Inc.
    Inventors: Chung-Jr Huang, Xiaoming Yang
  • Publication number: 20220251205
    Abstract: The invention provides compositions of anti-PD-1 antibodies or antigen-binding fragments thereof with less than or equal to about 3.0% oxidation of Met105 in the CDRH3 heavy chain region, and methods of obtaining the purified compositions. The invention also provides compositions comprising anti-PD-1 antibody main species and acidic species thereof, wherein the amount of acidic species is about 1.0-12.0%.
    Type: Application
    Filed: January 27, 2022
    Publication date: August 11, 2022
    Applicant: Merck Sharp & Dohme Corp.
    Inventors: Mark A. Brower, Chung-Jr Huang, Brian Wai Kwan, William Nicholas Napoli, Bhumit A. Patel, Nuno J. Dos Santos Pinto, Douglas Dennis Richardson, II, Sen Xu
  • Publication number: 20220081700
    Abstract: The present invention relates to methods of upregulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture by manipulating the mannose to total hexose ratio in the cell culture media formulation.
    Type: Application
    Filed: November 22, 2021
    Publication date: March 17, 2022
    Applicant: Amgen Inc.
    Inventors: Chung-Jr HUANG, Xiaoming YANG
  • Publication number: 20210268092
    Abstract: The present invention relates to a probiotic cell transformed with a construct suitable to overexpress and display on the surface of the probiotic cell a fusion protein comprising at least a portion of a transport protein coupled to at least a portion of one or more antigenic proteins or peptides. Probiotic-derived vesicles displaying this fusion protein as well as methods of inducing an immune response using the probiotic cells or vesicles are also disclosed.
    Type: Application
    Filed: December 31, 2020
    Publication date: September 2, 2021
    Inventors: David A. Putnam, Joseph Rosenthal, Chung-Jr Huang, Matthew DeLisa, Susana Mendez
  • Publication number: 20210164010
    Abstract: The present invention relates to methods of upregulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture by manipulating the mannose to total hexose ratio in the cell culture media formulation.
    Type: Application
    Filed: December 14, 2020
    Publication date: June 3, 2021
    Applicant: Amgen Inc.
    Inventors: Chung-Jr HUANG, Xiaoming YANG
  • Patent number: 10918706
    Abstract: The present invention relates to a probiotic cell transformed with a construct suitable to overexpress and display on the surface of the probiotic cell a fusion protein comprising at least a portion of a transport protein coupled to at least a portion of one or more antigenic proteins or peptides. Probiotic-derived vesicles displaying this fusion protein as well as methods of inducing an immune response using the probiotic cells or vesicles are also disclosed.
    Type: Grant
    Filed: November 26, 2019
    Date of Patent: February 16, 2021
    Assignee: Cornell University
    Inventors: David A. Putnam, Joseph Rosenthal, Chung-Jr Huang, Matthew DeLisa, Susana Mendez
  • Patent number: 10894972
    Abstract: The present invention relates to methods of upregulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture by manipulating the mannose to total hexose ratio in the cell culture media formulation.
    Type: Grant
    Filed: August 9, 2019
    Date of Patent: January 19, 2021
    Assignee: Amgen Inc.
    Inventors: Chung-Jr Huang, Xiaoming Yang
  • Publication number: 20200131518
    Abstract: Provided herein are methods of modulating the levels of total afucosylated (TAF) glycoforms of a recombinant glycosylated protein produced by glycosylation-competent cells in a cell culture. In exemplary embodiments, the methods comprise maintaining the cell culture at an initial set point pH for an initial cell culture period. Related compositions comprising glycosylated proteins and TAF glycoforms thereof are also provided herein.
    Type: Application
    Filed: March 14, 2018
    Publication date: April 30, 2020
    Inventors: Chung-jr Huang, Prince Bhebe, Madiha Khurshid, Li Zhang
  • Publication number: 20200093910
    Abstract: The present invention relates to a probiotic cell transformed with a construct suitable to overexpress and display on the surface of the probiotic cell a fusion protein comprising at least a portion of a transport protein coupled to at least a portion of one or more antigenic proteins or peptides. Probiotic-derived vesicles displaying this fusion protein as well as methods of inducing an immune response using the probiotic cells or vesicles are also disclosed.
    Type: Application
    Filed: November 26, 2019
    Publication date: March 26, 2020
    Inventors: David A. Putnam, Joseph Rosenthal, Chung-Jr Huang, Matthew DeLisa, Susana Mendez
  • Patent number: 10537627
    Abstract: The present invention relates to a probiotic cell transformed with a construct suitable to overexpress and display on the surface of the probiotic cell a fusion protein comprising at least a portion of a transport protein coupled to at least a portion of one or more antigenic proteins or peptides. Probiotic-derived vesicles displaying this fusion protein as well as methods of inducing an immune response using the probiotic cells or vesicles are also disclosed.
    Type: Grant
    Filed: September 20, 2017
    Date of Patent: January 21, 2020
    Assignee: Cornell University
    Inventors: David A. Putnam, Joseph Rosenthal, Chung-Jr Huang, Matthew DeLisa, Susana Mendez
  • Publication number: 20190360016
    Abstract: The present invention relates to methods of upregulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture by manipulating the mannose to total hexose ratio in the cell culture media formulation.
    Type: Application
    Filed: August 9, 2019
    Publication date: November 28, 2019
    Applicant: AMGEN INC.
    Inventors: Chung-Jr HUANG, Xiaoming YANG
  • Patent number: 10421987
    Abstract: The present invention relates to methods of upregulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture by manipulating the mannose to total hexose ratio in the cell culture media formulation.
    Type: Grant
    Filed: July 18, 2018
    Date of Patent: September 24, 2019
    Assignee: Amgen Inc.
    Inventors: Chung-Jr Huang, Xiaoming Yang
  • Patent number: 10184143
    Abstract: The present invention relates to methods of upregulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture by manipulating the mannose to total hexose ratio in the cell culture media formulation.
    Type: Grant
    Filed: October 4, 2016
    Date of Patent: January 22, 2019
    Assignee: Amgen Inc.
    Inventors: Chung-Jr Huang, Xiaoming Yang
  • Publication number: 20190002946
    Abstract: The present invention relates to methods of upregulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture by manipulating the mannose to total hexose ratio in the cell culture media formulation.
    Type: Application
    Filed: July 18, 2018
    Publication date: January 3, 2019
    Applicant: AMGEN INC.
    Inventors: Chung-Jr HUANG, Xiaoming YANG
  • Publication number: 20180071377
    Abstract: The present invention relates to a probiotic cell transformed with a construct suitable to overexpress and display on the surface of the probiotic cell a fusion protein comprising at least a portion of a transport protein coupled to at least a portion of one or more antigenic proteins or peptides. Probiotic-derived vesicles displaying this fusion protein as well as methods of inducing an immune response using the probiotic cells or vesicles are also disclosed.
    Type: Application
    Filed: September 20, 2017
    Publication date: March 15, 2018
    Inventors: David A. Putnam, Joseph Rosenthal, Chung-Jr Huang, Matthew DeLisa, Susana Mendez
  • Patent number: 9808517
    Abstract: The present invention relates to a probiotic cell transformed with a construct suitable to overexpress and display on the surface of the probiotic cell a fusion protein comprising at least a portion of a transport protein coupled to at least a portion of one or more antigenic proteins or peptides. Probiotic-derived vesicles displaying this fusion protein as well as methods of inducing an immune response using the probiotic cells or vesicles are also disclosed.
    Type: Grant
    Filed: March 13, 2013
    Date of Patent: November 7, 2017
    Assignee: Cornell University
    Inventors: David Putnam, Joseph Rosenthal, Chung-Jr Huang, Matthew Delisa, Susana Mendez
  • Publication number: 20170088874
    Abstract: The present invention relates to methods of upregulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture by manipulating the mannose to total hexose ratio in the cell culture media formulation.
    Type: Application
    Filed: October 4, 2016
    Publication date: March 30, 2017
    Applicant: AMGEN INC.
    Inventors: Chung-Jr HUANG, Xiaoming YANG
  • Patent number: 9481901
    Abstract: The present invention relates to methods of upregulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture by manipulating the mannose to total hexose ratio in the cell culture media formulation.
    Type: Grant
    Filed: May 29, 2014
    Date of Patent: November 1, 2016
    Inventors: Chung-Jr Huang, Xiaoming Yang
  • Publication number: 20150056246
    Abstract: The present invention relates to a probiotic cell transformed with a construct suitable to overexpress and display on the surface of the probiotic cell a fusion protein comprising at least a portion of a transport protein coupled to at least a portion of one or more antigenic proteins or peptides. Probiotic-derived vesicles displaying this fusion protein as well as methods of inducing an immune response using the probiotic cells or vesicles are also disclosed.
    Type: Application
    Filed: March 13, 2013
    Publication date: February 26, 2015
    Inventors: David A. Putnam, Joseph Rosenthal, Chung-Jr Huang, Matthew Delisa, Susana Mendez