Abstract: An improved gauze structure capable of separating inhalation and exhalation includes: an outer layer member, a top and a bottom portions thereof are formed with at least one first through hole, a center at the bottom portion is formed with a first front hole; an inner layer member, a top and a bottom portions thereof are formed with at least one second through hole, a center at the bottom portion is formed with a second front hole, at least one fastening unit is disposed at two sides; an inner mask member, used for covering a mouth, and formed with at least one third through hole and a connection part; and at least two air valves, disposed corresponding to the first and the second through holes at the top and bottom portions of the outer and the inner layer members and the third through hole of the inner mask member.

Abstract: Disclosed herein is a hairpin probe for detecting a target pathogenic microorganism in a sample. The hairpin probe includes a microbead and an oligonucleotide having its 3?-end coupled to the microbead. The oligonucleotide includes, from 5? to 3?, a Tag sequence hybridizable to a specific identification sequence of the pathogenic microorganism, an internal control sequence having at least four words each having 4 nucleotides with a 75% AT-content, an anti-Tag sequence being a reverse complement of the Tag sequence, and a tail having at least two consecutive thymidine residues. The Tag and anti-Tag sequences are operable to form a stem of the hairpin probe with the internal control sequence being a loop. Also disclosed herein are, a kit including the hairpin probe and a method for using the kit in the detection of a target pathogenic microorganism.