Patents by Inventor Chungen QIAN

Chungen QIAN has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240307872
    Abstract: The present invention relates to a microfluidic chip, a reagent output structure thereof comprising a reagent storage chamber, a first centrifugal-force flow channel and a time delay unit. The time delay unit comprises a first diverting flow channel, a capillary-force flow channel, a second diverting flow channel and a second centrifugal-force flow channel. The first centrifugal-force flow channel extends away from the centre of rotation. The other end of the first diverting flow channel is in communication with one end of the capillary-force flow channel, the capillary-force flow channel extends towards the centre of rotation, the other end of the capillary-force flow channel is in communication with one end of the second diverting flow channel, the other end of the second diverting flow channel is in communication with the second centrifugal-force flow channel, and the second centrifugal-force flow channel extends away from the centre of rotation.
    Type: Application
    Filed: January 19, 2022
    Publication date: September 19, 2024
    Inventors: Chungen QIAN, Ming HUANG, Bifeng LIU, Peng CHEN, Yujin XIAO, Kunhui HU
  • Publication number: 20240173716
    Abstract: The present disclosure discloses a microfluidic chip, a system and a method for automatic separation and detection of circulating tumor cells. The microfluidic chip includes a density gradient centrifugation assembly, a cell capture assembly, and a reagent storage assembly. The density gradient centrifugation assembly is configured to carry out density gradient centrifugation on a whole blood sample to achieve separation and obtain a plasma layer, a monocyte layer, a Ficoll solution layer, and an erythrocyte and granulocyte layer. The cell capture assembly includes a capture channel, a capture inlet, and a capture outlet. The capture inlet and the capture outlet are in communication with the capture channel. An inner wall of the capture channel is provided with capture holes and negative-pressure capture chambers. Each capture hole is in communication with one negative-pressure capture chamber correspondingly.
    Type: Application
    Filed: January 19, 2022
    Publication date: May 30, 2024
    Inventors: Chungen QIAN, Shunji LI, Bifeng LIU, Peng CHEN, Liqiang WU, Chang ZOU
  • Publication number: 20230251250
    Abstract: The present disclosure relates to a microfluidic immunoassay chip and a microfluidic line immunoassay method. The microfluidic immunoassay chip includes a loading cell, a reaction and detection cell, a washing cell, an enzyme storing cell, a substrate cell, and a termination cell, and a waste liquid cell. A detection membrane strip is disposed in the reaction and detection cell and coated with a capture antigen or a capture antibody.
    Type: Application
    Filed: August 20, 2021
    Publication date: August 10, 2023
    Inventors: Yanping ZHENG, Xiaoyu CHENG, Daoshun WEI, Chungen QIAN, Kunhui HU
  • Publication number: 20230241614
    Abstract: A droplet microfluidic chip and a method for producing microdroplets are disclosed. The droplet microfluidic chip includes at least one droplet-producing unit. The droplet-producing unit includes a dispersion phase chamber, a quantitation chamber, a capillary nozzle, and a continuous phase chamber. The droplet microfluidic chip has a rotation center. The dispersion phase chamber is provided with a loading hole configured to introduce a dispersion phase liquid. The quantitation chamber is in communication with the dispersion phase chamber and further away from the rotation center than the dispersion phase chamber. The capillary nozzle is further away from the rotation center than the quantitation chamber. One end of the capillary nozzle is in communication with the quantitation chamber, and the capillary nozzle is extended from the joining end in a direction away from the rotation center.
    Type: Application
    Filed: August 3, 2021
    Publication date: August 3, 2023
    Inventors: Shunji LI, Xiaoyu CHENG, Bifeng LIU, Chungen QIAN, Kunhui HU
  • Publication number: 20230220052
    Abstract: The disclosure relates to the technical field of virus detection, in particular, to an IgA antibody and a kit capable of specifically recognizing RBD protein. The antibody can be used as a calibrator of IgA antibody against the RBD protein of SARS-CoV-2.
    Type: Application
    Filed: November 20, 2020
    Publication date: July 13, 2023
    Inventors: Fangming CHENG, Xiaotao LIN, Zhisheng DANG, Liqiang WU, Chungen QIAN, Kunhui HU
  • Patent number: 11619629
    Abstract: Provided in the present invention are a magnetic nanosphere coated with modified cardiolipin, and manufacturing method thereof. The magnetic nanosphere coated with modified cardiolipin comprises a modified cardiolipin, a biotin derivative, and a streptavidin magnetic bead. The modified cardiolipin is coupled to the biotin derivative via an —NH—CO structure. The streptavidin magnetic bead is a magnetic nanosphere coupled to streptavidin, and the biotin derivative is coupled to the streptavidin.
    Type: Grant
    Filed: June 30, 2016
    Date of Patent: April 4, 2023
    Assignee: SHENZHEN YHLO BIOTECH CO., LTD.
    Inventors: Fuzhen Xia, Dingbiao Zou, Gang Wang, Xiaoqin Ye, Chungen Qian
  • Publication number: 20220389064
    Abstract: Disclosed are a recombinant RBD protein of SARS-CoV-2, and a method for preparing the same. The recombinant RBD protein has a signal polypeptide sequence, an RBD protein sequence, a transmembrane domain sequence and a tag sequence in order from N-terminus to C-terminus. The recombinant RBD protein may be expressed on cell membranes, which facilitates protein stability, enrichment and detection.
    Type: Application
    Filed: November 20, 2020
    Publication date: December 8, 2022
    Inventors: Xiaotao LIN, Qiulian RUAN, Zhihao XING, Chungen QIAN, Kunhui HU
  • Publication number: 20220372083
    Abstract: Provided are a recombinant N protein of SARS-CoV-2 and methods for preparing the same and for purifying the same. The recombinant N protein of SARS-CoV-2 has an N protein sequence, wherein each of both ends of the N protein sequence are linked to an oligolysine fragment. An expression method for the recombinant N protein includes: a. providing a sample including the recombinant N protein; and b. performing cation exchange chromatography resin purification treatment at least once, and collecting breakthrough substance. The method may effectively improve the expression purity of the recombinant N protein.
    Type: Application
    Filed: November 20, 2020
    Publication date: November 24, 2022
    Inventors: Xiaotao LIN, Chunlei LUO, Sai ZHANG, Chungen QIAN, Kunhui HU
  • Publication number: 20220220163
    Abstract: A recombinant deamidated gliadin polypeptide antigen, a recombinant antigen-expressing gene, a recombinant expression vector, a preparation method therefor, and an application thereof. The recombinant deamidated gliadin polypeptide antigen comprises a DGP-1 peptide, an interleukin 15 protein, and a DGP-2 peptide. The amino acid sequence of the DGP-1 peptide is as shown in SEQ ID NO. 1, and the amino acid sequence of the DGP-2 peptide is as shown in SEQ ID NO 2. The recombinant deamidated gliadin polypeptide antigen has good sensitivity, high specificity, and low preparation costs in the detection of celiac disease, allows determination of IgG-DGP antibodies in serum, and especially enables diagnosis for potential patients with celiac disease among patients with IgA deficiency and infant population.
    Type: Application
    Filed: December 24, 2019
    Publication date: July 14, 2022
    Inventors: Liang ZHENG, Chunlei LUO, Chungen QIAN, Kunhui HU
  • Publication number: 20220144912
    Abstract: Provided are a leptin immunogen, hybridoma cells, a monoclonal antibody, a polyclonal antibody and use thereof. The leptin immunogen comprises a polypeptide having an amino acid sequence as shown in SEQ ID NO: 1. The anti-leptin monoclonal antibody prepared with the leptin immunogen has good specificity and high sensitivity.
    Type: Application
    Filed: December 24, 2019
    Publication date: May 12, 2022
    Inventors: Mi ZHOU, Yujian LIN, Chungen QIAN, Kunhui HU
  • Publication number: 20210231649
    Abstract: Provided in the present invention are a magnetic nanosphere coated with modified cardiolipin, and manufacturing method thereof. The magnetic nanosphere coated with modified cardiolipin comprises a modified cardiolipin, a biotin derivative, and a streptavidin magnetic bead. The modified cardiolipin is coupled to the biotin derivative via an —NH—CO structure. The streptavidin magnetic bead is a magnetic nanosphere coupled to streptavidin, and the biotin derivative is coupled to the streptavidin.
    Type: Application
    Filed: June 30, 2016
    Publication date: July 29, 2021
    Inventors: Fuzhen XIA, Dingbiao ZOU, Gang WANG, Xiaoqin YE, Chungen QIAN
  • Publication number: 20200309770
    Abstract: Provided are a chemiluminescence immunoassay kit for adiponectin, and a preparation method and a use thereof. The chemiluminescence immunoassay kit for adiponectin comprises: a solid carrier coated with an adiponectin monoclonal antibody; and an adiponectin monoclonal antibody marked with a chemiluminescence marker.
    Type: Application
    Filed: July 6, 2016
    Publication date: October 1, 2020
    Inventors: Fuzhen XIA, Chungen QIAN, Gang WANG, Liang ZHU, Yuxi HE
  • Patent number: 10745425
    Abstract: Disclosed a chemiluminescence enhancer and a chemiluminescence immunodetection kit. The chemiluminescence enhancer is specifically a betaine and can be used for enhancing the luminance of a luminescent acridine substance. The chemiluminescence immunodetection kit comprising the chemiluminescence enhancer and a luminescent acridine substance features greatly enhanced luminescence signals and high detection sensibility, and can be widely applied to fields such as clinical diagnoses, scientific research, and sanitation analysis.
    Type: Grant
    Filed: July 1, 2016
    Date of Patent: August 18, 2020
    Assignee: SHENZHEN YHLO BIOTECH CO., LTD.
    Inventors: Kunhui Hu, Gang Wang, Fuzhen Xia, Chungen Qian, Liang Zhu, Dingbiao Zou
  • Publication number: 20190323969
    Abstract: The present invention discloses a zinc transporter 8 antibody chemiluminescence immunoassay kit, including a magnetic microparticle working solution coated with purified zinc transporter 8, a purified zinc transporter 8 working solution labeled with acridinium ester, a zinc transporter 8 antibody calibrator, a chemiluminescent pre-excitation solution and a chemiluminescent excitation solution. In addition, the present invention also discloses a method for preparing the zinc transporter 8 antibody chemiluminescence immunoassay kit.
    Type: Application
    Filed: April 13, 2017
    Publication date: October 24, 2019
    Applicant: SHENZHEN YHLO BIOTECH CO., LTD.
    Inventors: Lin HE, Qiaohong CHEN, Yonghong YANG, Fuzhen XIA, Chungen QIAN, Xing LIU
  • Publication number: 20190309030
    Abstract: Disclosed are an acridine marker conjugate and a preparation method therefor and a chemiluminescent kit. The acridine marker conjugate comprises an acridine substitute, a carrier protein and a protein to be marked sequentially connected. The carrier protein is a protein containing carboxyl and amino, a modified protein, a polypeptide or a modified polypeptide; and the protein to be marked is a protein containing amino, a modified protein, a polypeptide or a modified polypeptide.
    Type: Application
    Filed: July 5, 2016
    Publication date: October 10, 2019
    Inventors: Fuzhen XIA, Taoxu LIU, Liang ZHU, Chengyong XIAO, Chungen QIAN
  • Publication number: 20190153003
    Abstract: Disclosed a chemiluminescence enhancer and a chemiluminescence immunodetection kit. The chemiluminescence enhancer is specifically a betaine and can be used for enhancing the luminance of a luminescent acridine substance. The chemiluminescence immunodetection kit comprising the chemiluminescence enhancer and a luminescent acridine substance features greatly enhanced luminescence signals and high detection sensibility, and can be widely applied to fields such as clinical diagnoses, scientific research, and sanitation analysis.
    Type: Application
    Filed: July 1, 2016
    Publication date: May 23, 2019
    Inventors: Kunhui HU, Gang WANG, Fuzhen XIA, Chungen QIAN, Liang ZHU, Dingbiao ZOU
  • Patent number: 9139766
    Abstract: An enhancing reagent for enhancing chemiluminescence of 1,2-dioxetane compounds and a method for using the enhancing reagent to enhance the chemiluminescence are provided, in which the enhancing reagent contains an alkyl bis-quaternary ammonium salt of Formula I. A chemiluminescent composition with a 1,2-dioxetane compound as a substrate and a kit thereof are further provided, which contain a 1,2-dioxetane compound and an alkyl bis-quaternary ammonium salt of Formula I.
    Type: Grant
    Filed: October 10, 2013
    Date of Patent: September 22, 2015
    Assignee: SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS CO., LTD.
    Inventors: Chungen Qian, Yuping Zhang
  • Patent number: 8940512
    Abstract: Methods for synthesizing an ALP conjugate are provided. The methods may include activating a carboxyl group of the ALP with a carbodiimide, to generate an active ester; and adding the substance to be conjugated such that a synthetic reaction occurs between the active ester and the substance to be conjugated, to generate an ALP conjugate.
    Type: Grant
    Filed: June 2, 2011
    Date of Patent: January 27, 2015
    Assignee: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
    Inventors: Chungen Qian, Ke Li, Yuping Zhang
  • Patent number: 8883514
    Abstract: A enhancing reagent for enhancing chemiluminescence of 1,2-dioxetane compounds and a method for using the enhancing reagent to enhance chemiluminescence are provided, in which the enhancing reagent contains a multi-alkyl quaternary ammonium salt of Formula I. A chemiluminescent composition with a 1,2-dioxetane compound as a substrate and a kit thereof are further provided, which contain a 1,2-dioxetane compound and a multi-alkyl quaternary ammonium salt of Formula I.
    Type: Grant
    Filed: June 29, 2011
    Date of Patent: November 11, 2014
    Assignee: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
    Inventors: Chungen Qian, Yuping Zhang
  • Patent number: 8753858
    Abstract: The present disclosure relates to stabilizers for alkaline phosphatase or conjugates thereof, a process for preparing a stabilizer, and a method for stabilizing alkaline phosphatase or conjugates thereof with a stabilizer. The present disclosure also relates to a reagent of alkaline phosphatase or conjugates thereof as well as to a process for preparing the same. In another aspect, the present disclosure relates to a kit comprising the stabilizers disclosed herein and alkaline phosphatase or conjugates thereof. The stabilizer disclosed herein can stabilize alkaline phosphatase or conjugates thereof for a prolonged period of time, extending their shelf-life.
    Type: Grant
    Filed: December 29, 2010
    Date of Patent: June 17, 2014
    Assignee: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
    Inventors: Chungen Qian, Yuping Zhang