Abstract: The present disclosure relates to a microfluidic immunoassay chip and a microfluidic line immunoassay method. The microfluidic immunoassay chip includes a loading cell, a reaction and detection cell, a washing cell, an enzyme storing cell, a substrate cell, and a termination cell, and a waste liquid cell. A detection membrane strip is disposed in the reaction and detection cell and coated with a capture antigen or a capture antibody.

Abstract: A droplet microfluidic chip and a method for producing microdroplets are disclosed. The droplet microfluidic chip includes at least one droplet-producing unit. The droplet-producing unit includes a dispersion phase chamber, a quantitation chamber, a capillary nozzle, and a continuous phase chamber. The droplet microfluidic chip has a rotation center. The dispersion phase chamber is provided with a loading hole configured to introduce a dispersion phase liquid. The quantitation chamber is in communication with the dispersion phase chamber and further away from the rotation center than the dispersion phase chamber. The capillary nozzle is further away from the rotation center than the quantitation chamber. One end of the capillary nozzle is in communication with the quantitation chamber, and the capillary nozzle is extended from the joining end in a direction away from the rotation center.

Abstract: The disclosure relates to the technical field of virus detection, in particular, to an IgA antibody and a kit capable of specifically recognizing RBD protein. The antibody can be used as a calibrator of IgA antibody against the RBD protein of SARS-CoV-2.

Abstract: Provided in the present invention are a magnetic nanosphere coated with modified cardiolipin, and manufacturing method thereof. The magnetic nanosphere coated with modified cardiolipin comprises a modified cardiolipin, a biotin derivative, and a streptavidin magnetic bead. The modified cardiolipin is coupled to the biotin derivative via an —NH—CO structure. The streptavidin magnetic bead is a magnetic nanosphere coupled to streptavidin, and the biotin derivative is coupled to the streptavidin.

Abstract: Disclosed are a recombinant RBD protein of SARS-CoV-2, and a method for preparing the same. The recombinant RBD protein has a signal polypeptide sequence, an RBD protein sequence, a transmembrane domain sequence and a tag sequence in order from N-terminus to C-terminus. The recombinant RBD protein may be expressed on cell membranes, which facilitates protein stability, enrichment and detection.

Abstract: Provided are a recombinant N protein of SARS-CoV-2 and methods for preparing the same and for purifying the same. The recombinant N protein of SARS-CoV-2 has an N protein sequence, wherein each of both ends of the N protein sequence are linked to an oligolysine fragment. An expression method for the recombinant N protein includes: a. providing a sample including the recombinant N protein; and b. performing cation exchange chromatography resin purification treatment at least once, and collecting breakthrough substance. The method may effectively improve the expression purity of the recombinant N protein.

Abstract: A recombinant deamidated gliadin polypeptide antigen, a recombinant antigen-expressing gene, a recombinant expression vector, a preparation method therefor, and an application thereof. The recombinant deamidated gliadin polypeptide antigen comprises a DGP-1 peptide, an interleukin 15 protein, and a DGP-2 peptide. The amino acid sequence of the DGP-1 peptide is as shown in SEQ ID NO. 1, and the amino acid sequence of the DGP-2 peptide is as shown in SEQ ID NO 2. The recombinant deamidated gliadin polypeptide antigen has good sensitivity, high specificity, and low preparation costs in the detection of celiac disease, allows determination of IgG-DGP antibodies in serum, and especially enables diagnosis for potential patients with celiac disease among patients with IgA deficiency and infant population.

Abstract: Provided are a leptin immunogen, hybridoma cells, a monoclonal antibody, a polyclonal antibody and use thereof. The leptin immunogen comprises a polypeptide having an amino acid sequence as shown in SEQ ID NO: 1. The anti-leptin monoclonal antibody prepared with the leptin immunogen has good specificity and high sensitivity.

Abstract: Provided in the present invention are a magnetic nanosphere coated with modified cardiolipin, and manufacturing method thereof. The magnetic nanosphere coated with modified cardiolipin comprises a modified cardiolipin, a biotin derivative, and a streptavidin magnetic bead. The modified cardiolipin is coupled to the biotin derivative via an —NH—CO structure. The streptavidin magnetic bead is a magnetic nanosphere coupled to streptavidin, and the biotin derivative is coupled to the streptavidin.

Abstract: Provided are a chemiluminescence immunoassay kit for adiponectin, and a preparation method and a use thereof. The chemiluminescence immunoassay kit for adiponectin comprises: a solid carrier coated with an adiponectin monoclonal antibody; and an adiponectin monoclonal antibody marked with a chemiluminescence marker.

Abstract: Disclosed a chemiluminescence enhancer and a chemiluminescence immunodetection kit. The chemiluminescence enhancer is specifically a betaine and can be used for enhancing the luminance of a luminescent acridine substance. The chemiluminescence immunodetection kit comprising the chemiluminescence enhancer and a luminescent acridine substance features greatly enhanced luminescence signals and high detection sensibility, and can be widely applied to fields such as clinical diagnoses, scientific research, and sanitation analysis.

Abstract: The present invention discloses a zinc transporter 8 antibody chemiluminescence immunoassay kit, including a magnetic microparticle working solution coated with purified zinc transporter 8, a purified zinc transporter 8 working solution labeled with acridinium ester, a zinc transporter 8 antibody calibrator, a chemiluminescent pre-excitation solution and a chemiluminescent excitation solution. In addition, the present invention also discloses a method for preparing the zinc transporter 8 antibody chemiluminescence immunoassay kit.

Abstract: Disclosed are an acridine marker conjugate and a preparation method therefor and a chemiluminescent kit. The acridine marker conjugate comprises an acridine substitute, a carrier protein and a protein to be marked sequentially connected. The carrier protein is a protein containing carboxyl and amino, a modified protein, a polypeptide or a modified polypeptide; and the protein to be marked is a protein containing amino, a modified protein, a polypeptide or a modified polypeptide.

Abstract: Disclosed a chemiluminescence enhancer and a chemiluminescence immunodetection kit. The chemiluminescence enhancer is specifically a betaine and can be used for enhancing the luminance of a luminescent acridine substance. The chemiluminescence immunodetection kit comprising the chemiluminescence enhancer and a luminescent acridine substance features greatly enhanced luminescence signals and high detection sensibility, and can be widely applied to fields such as clinical diagnoses, scientific research, and sanitation analysis.

Abstract: An enhancing reagent for enhancing chemiluminescence of 1,2-dioxetane compounds and a method for using the enhancing reagent to enhance the chemiluminescence are provided, in which the enhancing reagent contains an alkyl bis-quaternary ammonium salt of Formula I. A chemiluminescent composition with a 1,2-dioxetane compound as a substrate and a kit thereof are further provided, which contain a 1,2-dioxetane compound and an alkyl bis-quaternary ammonium salt of Formula I.

Abstract: Methods for synthesizing an ALP conjugate are provided. The methods may include activating a carboxyl group of the ALP with a carbodiimide, to generate an active ester; and adding the substance to be conjugated such that a synthetic reaction occurs between the active ester and the substance to be conjugated, to generate an ALP conjugate.

Abstract: A enhancing reagent for enhancing chemiluminescence of 1,2-dioxetane compounds and a method for using the enhancing reagent to enhance chemiluminescence are provided, in which the enhancing reagent contains a multi-alkyl quaternary ammonium salt of Formula I. A chemiluminescent composition with a 1,2-dioxetane compound as a substrate and a kit thereof are further provided, which contain a 1,2-dioxetane compound and a multi-alkyl quaternary ammonium salt of Formula I.

Abstract: The present disclosure relates to stabilizers for alkaline phosphatase or conjugates thereof, a process for preparing a stabilizer, and a method for stabilizing alkaline phosphatase or conjugates thereof with a stabilizer. The present disclosure also relates to a reagent of alkaline phosphatase or conjugates thereof as well as to a process for preparing the same. In another aspect, the present disclosure relates to a kit comprising the stabilizers disclosed herein and alkaline phosphatase or conjugates thereof. The stabilizer disclosed herein can stabilize alkaline phosphatase or conjugates thereof for a prolonged period of time, extending their shelf-life.

Abstract: An enhancing reagent for enhancing chemiluminescence of 1,2-dioxetane compounds and a method for using the enhancing reagent to enhance the chemiluminescence are provided, in which the enhancing reagent contains an alkyl bis-quaternary ammonium salt of Formula I. A chemiluminescent composition with a 1,2-dioxetane compound as a substrate and a kit thereof are further provided, which contain a 1,2-dioxetane compound and an alkyl bis-quaternary ammonium salt of Formula I.

Abstract: A enhancing reagent for enhancing chemiluminescence of 1,2-dioxetane compounds and a method for using the enhancing reagent to enhance the chemiluminescence are provided, in which the enhancing reagent contains an alkyl bis-quaternary ammonium salt of Formula I. A chemiluminescent composition with a 1,2-dioxetane compound as a substrate and a kit thereof are further provided, which contain a 1,2-dioxetane compound and an alkyl bis-quaternary ammonium salt of Formula I.