Abstract: Processing methods and apparatus for increasing a reaction chamber batch size. Such a method of processing deposition substrates (e.g., wafers), involves conducting a deposition on a first portion of a batch of deposition wafers in a reaction chamber, conducting an interval conditioning reaction chamber purge to remove defects generated by the wafer processing from the reaction chamber; and following the interval conditioning mid-batch reaction chamber purge, conducting the deposition on another portion of the batch of wafers in the reaction chamber. The interval conditioning reaction chamber purge is conducted prior to exceeding a baseline for acceptable defect (e.g., particle) generation in the chamber and is performed while no wafers are positioned in the reaction chamber.

Abstract: The invention relates to engineered terminal deoxynucleotidyl transferase (TdT) enzymes or the homologous amino acid sequence of Pol?, Pol?, Pol?, and Pol? of any species or the homologous amino acid sequence of X family polymerases of any species and their use in a method of nucleic acid synthesis, to methods of synthesizing nucleic acids, and to the use of kits comprising said enzymes in a method of nucleic acid synthesis. The invention also relates to the use of new terminal deoxynucleotidyl transferases and 3?-blocked nucleoside triphosphates in a method of template independent nucleic acid synthesis.

Abstract: Provided herein are methods of cell-free protein synthesis, optimised cell-free protein synthesis (CFPS) reagents and the post translational modification of the expressed proteins, to increase protein expression yields. The methods are applicable to protein expression on a microfluidic device having hydrophobic surfaces by merging droplets on the device in order to screen a selection of expression and post translational modification compositions in parallel.

Abstract: Processing methods and apparatus for increasing a reaction chamber batch size. Such a method of processing deposition substrates (e.g., wafers), involves conducting a deposition on a first portion of a batch of deposition wafers in a reaction chamber, conducting an interval conditioning reaction chamber purge to remove defects generated by the wafer processing from the reaction chamber; and following the interval conditioning mid-batch reaction chamber purge, conducting the deposition on another portion of the batch of wafers in the reaction chamber. The interval conditioning reaction chamber purge is conducted prior to exceeding a baseline for acceptable defect (e.g., particle) generation in the chamber and is performed while no wafers are positioned in the reaction chamber.

Abstract: Provided herein are methods, and compositions for the detection and analysis of biomolecule interactions a microfluidic device. The detection and analysis occurs in aqueous droplets having a first surfactant within an oil layer having a second surfactant.

Abstract: A non-volatile memory device and its manufacturing method are provided. The non-volatile memory device includes a substrate and a plurality of first floating gates and a plurality of second floating gates formed on the substrate. The substrate includes a center region and two border regions located on opposite sides of the center region. The center region and two border regions are located in an array region. The first floating gates are located in the center region, and the second floating gates are located in one of the border regions. Each of the first floating gates has a first width, and each of the second floating gates has a second width less than the first width. There is a first spacing between the first floating gates, and there is a second spacing which is greater than the first spacing between the second floating gates.

Abstract: The invention relates to engineered terminal deoxynucleotidyl transferase (TdT) enzymes or the homologous amino acid sequence of Pol?, Pol?, Pol?, and Pol? of any species or the homologous amino acid sequence of X family polymerases of any species and uses thereof.

Abstract: The present disclosure provides a private network and edge application provisioning management system and a method thereof, which provides a construction of enterprise private cloud platform and a process of management standardization, so that a dedicated cloud operating environment can be provided for enterprise customers, and special software application services can further be established and managed. A remote registration mechanism, an enterprise private network establishment mechanism, and an edge application activation mechanism be proposed in the present disclosure. In this way, edge computing capabilities in the enterprise private network environment can be provided, which can not only reduce application service latency time, but also network transmission does not need to go through the Internet, thus having the advantages of avoiding data leakage and improving security. The present disclosure also provides a computer-readable medium for executing the method of the present disclosure.

Abstract: The invention provided herein relates to methods for measuring protein aggregation and stability. The methods may be applied to characterising cell-free protein synthesis reactions.

Abstract: A system and a method for managing a virtual network function (VNF) and a multi-access edge computing (MEC) topology are provided. The method includes the following steps. A first VNF descriptor (VNFD) corresponding to a first VNF is received. According to the first VNFD, first provision data is generated. According to the first VNFD, first internal topology information of the first VNF is generated. According to the first provision data, the first VNF is instantiated to be provisioned. In response to provisioning the first VNF, a graphical user interface including the first internal topology information is output, and the first internal topology information includes a network component communicatively connected to the first VNF.

Abstract: A polyhydroxyalkanoate block copolymer includes a first segment containing a first monomeric unit represented by formula (I); and a second segment containing the first monomeric unit represented by formula (I), a second monomeric unit represented by formula (II), and a third monomeric unit represented by formula (III), The first segment and the second segment are arranged in blocks. A method for preparing the polyhydroxyalkanoate block copolymer is also disclosed.

Abstract: A method of reconstructing transcranial images using a dual-mode ultrasonic phased array includes steps of: controlling channels to emit energy toward an intracranial target point of a patient; respectively generating backscattered radiofrequency (RF) data by using the channels to receive backscattered energy reflected from the intracranial target; and reconstructing an acoustic distribution image based on those backscattered RF data in real-time. Compared with Pre-Treatment Ray Tracing Method, the present invention can display intracranial pressure distribution in real-time; compared with MR Thermometry, the present invention can be applied to low-energy applications without temperature change; and compared with Passive Cavitation Imaging, the present invention can stably present acoustic distribution images without relying on microbubbles.

Abstract: Provided herein are methods, and compositions for the real-time detection of protein synthesis. The methods are applicable to monitoring on a microfluidic device.

Abstract: A method for moving an aqueous droplet comprising providing an electrokinetic device including a first substrate having a matrix of electrodes, wherein each of the matrix electrodes is coupled to a thin film transistor, and wherein the matrix electrodes are overcoated with a functional coating comprising: a dielectric layer in contact with the matrix electrodes, a conformal layer in contact with the dielectric layer, and a hydrophobic layer in contact with the conformal layer; a second substrate comprising a top electrode; a spacer disposed between the first substrate and the second substrate and defining an electrokinetic workspace; and a voltage source operatively coupled to the matrix electrodes. The method further comprises disposing an aqueous droplet on a first matrix electrode; and providing a differential electrical potential between the first matrix electrode and a second matrix electrode with the voltage source, thereby moving the aqueous droplet.

Abstract: Disclosed is a method for the cell-free expression of peptides or proteins in a liquid filled digital microfluidic device. The droplets having the components required for cell-free protein expression can be manipulated by electrokinesis in order to enhance levels of protein expression in the droplets.

Abstract: The invention relates to the use of specific terminal deoxynucleotidyl transferase (TdT) enzymes or the homologous amino acid sequence of PoIµ, PoI?, PoI?, and PoI? of any species or the homologous amino acid sequence of X family polymerases of any species in a method of nucleic acid synthesis, to methods of synthesizing nucleic acids, and to the use of kits comprising said enzymes in a method of nucleic acid synthesis. The invention also relates to the use of terminal deoxynucleotidyl transferases or homologous enzymes and 3?-blocked nucleoside triphosphates in a method of template independent nucleic acid synthesis.

Abstract: A method for moving an aqueous droplet comprising providing an electrokinetic device including a first substrate having a matrix of electrodes, wherein each of the matrix electrodes is coupled to a thin film transistor, and wherein the matrix electrodes are overcoated with a functional coating comprising: a dielectric layer in contact with the matrix electrodes, a conformal layer in contact with the dielectric layer, and a hydrophobic layer in contact with the confornial layer; a second substrate comprising a top electrode; a spacer disposed between the first substrate and the second substrate and defining an electrokinetic workspace; and a voltage source operatively coupled to the niatrix electrodes. The method further comprises disposing an aqueous droplet on a first matrix electrode; and providing a differential electrical potential between the first matrix electrode and a second matrix electrode with the voltage source, thereby moving the aqueous droplet.

Abstract: A non-volatile memory device and its manufacturing method are provided. The non-volatile memory device includes a substrate and a plurality of first floating gates and a plurality of second floating gates formed on the substrate. The substrate includes a center region and two border regions located on opposite sides of the center region. The center region and two border regions are located in an array region. The first floating gates are located in the center region, and the second floating gates are located in one of the border regions. Each of the first floating gates has a first width, and each of the second floating gates has a second width less than the first width. There is a first spacing between the first floating gates, and there is a second spacing which is greater than the first spacing between the second floating gates.

Abstract: Disclosed an improved process for synthesising a nucleic acid strand using cycles of template independent enzyme extension. Having one or more of the amino groups on the base heterocyclic groups masked with protecting groups helps to prevent secondary structure in the extended strand, thereby improving access of the enzyme to the 3? OH terminus for extension.

Abstract: The invention provided herein relates to methods for protein synthesis, characterisation and purification on a digital microfluidic device.