Abstract: A viscosity-adjustable medium for use in separation methods is described. In particular, a block copolymer composition is described which exists as a low viscosity solution at a temperature outside of the temperature range of electrophoresis and which self-assembles into a gel-like polymer network at electrophoresis operating temperatures. A system is described for conducting molecular separation in a capillary column using the viscosity-adjustable medium. In addition, a method is also described for separating charged molecules.

Abstract: A device is described for electrophoretic molecular separation. This device has a channel formed on a hydrophobic surface thereof. The interior surface of the channel is rendered hydrophilic through successive oxidation and protophilic reactions. A cover extends over the channel to form a conduit which is dimensioned to accept a molecular separation media. The conduit is also positioned for application of an electric current along the length of the interior of the conduit which drives a sample through the molecular separation media. Systems and methods are also described for electrophoretic molecular separation.