Abstract: Provided herein are methods and systems useful for the determination and assignment of haplotypes for genetic loci.

Abstract: A graphics processing unit (GPU) may include a triangle setup engine (TSE) configured to determine coordinates of a triangle, rotate coordinates of the triangle based on an angle. To rotate the coordinates, the TSE generates coordinates of the triangle in a rotated domain, and determines coordinates of a bounding box in the rotated domain based on the coordinates of the triangle in the rotated domain. The TSE determines a first plurality of parallel scanlines in the rotated domain, and a second plurality of parallel scanlines in the rotated domain. The first and second pluralities of scanlines are perpendicular. The TSE determines whether the bounding box coordinates are located within two adjacent scanlines. If the bounding box coordinates are located within the two adjacent scanlines, the TSE removes the triangle from the scene.

Abstract: The present disclosure generally pertains to a multiplex method for analyzing samples comprising using polynucleotide amplification to produce amplified products wherein one or more target sequences are tagged with a non-interfering, non-canceling target-specific polynucleotide identification tag, pyrosequencing the amplified products through the non-canceling target-specific polynucleotide identification tag sequence to detect the presence of one or more specific polynucleotide identification tags. The presence of a specific polynucleotide identification tag being correlated with the presence of a specific target sequence.

Abstract: Methods are provided to determine the entire genomic region of a particular HLA locus including both intron and exons. The resultant consensus sequences provides linkage information between different exons, and produces the unique sequence from each of the two genes from the individual sample being typed. The sequence information in intron regions along with the exon sequences provides an accurate HLA haplotype.

Abstract: Methods are provided to determine the entire genomic region of a particular HLA locus including both intron and exons. The resultant consensus sequences provides linkage information between different exons, and produces the unique sequence from each of the two genes from the individual sample being typed. The sequence information in intron regions along with the exon sequences provides an accurate HLA haplotype.

Abstract: A graphics processing unit (GPU) may include a triangle setup engine (TSE) configured to determine coordinates of a triangle, rotate coordinates of the triangle based on an angle. To rotate the coordinates, the TSE generates coordinates of the triangle in a rotated domain, and determines coordinates of a bounding box in the rotated domain based on the coordinates of the triangle in the rotated domain. The TSE determines a first plurality of parallel scanlines in the rotated domain, and a second plurality of parallel scanlines in the rotated domain. The first and second pluralities of scanlines are perpendicular. The TSE determines whether the bounding box coordinates are located within two adjacent scanlines. If the bounding box coordinates are located within the two adjacent scanlines, the TSE removes the triangle from the scene.

Abstract: This disclosure describes an adaptive memory address scanning technique that defines an address scanning pattern, to be used for a particular surface, based on one or more properties of the surface. In addition, a number, shape, and arrangement of sub-primitives of a surface to process in parallel may be determined. In one example of the disclosure, a memory accessing method for graphics processing comprises, determining, by a graphics processing unit (GPU), properties of a surface, determining, by the GPU, a memory address scanning technique based on the determined properties of the surface, and performing, by the GPU, at least one of a read or a write of data associated with the surface in a memory based on the determined memory address scanning technique.

Abstract: Disclosed is a method for amplifying RNA from T and B-cell populations and using the amplified RNA products to evaluate the possible correlation between a normal or abnormal immune response and the development of a disease such as an autoimmune disease, cancer, diabetes, or heart disease.

Abstract: This disclosure relates to arbitration of different types of requests to access a cache. Features of this disclosure can be implemented in a graphics processing unit (GPU). In one embodiment, an arbiter can receive requests from a color processor and a depth processor and determine which of the received requests has the highest priority. The request with the highest priority can then be provided to the cache. The priority can be configurable. The arbiter can determine priority, for example, based on whether a location in the cache associated with a request is available, a weight associated with the request, a number of requests of a particular type processed by the arbiter, or any combination thereof.

Abstract: Methods are provided to determine the genomic sequence of the alleles at the HLA gene. The resultant sequences provide linkage information between different exons, and produces the unique sequence at each gene from the two alleles of the individual sample being typed. The sequence information provides an accurate HLA haplotype. Methods to decrease allele dropout during long range PCR reactions are also disclosed.

Abstract: This disclosure relates to arbitration of different types of requests to access a cache. Features of this disclosure can be implemented in a graphics processing unit (GPU). In one embodiment, an arbiter can receive requests from a color processor and a depth processor and determine which of the received requests has the highest priority. The request with the highest priority can then be provided to the cache. The priority can be configurable. The arbiter can determine priority, for example, based on whether a location in the cache associated with a request is available, a weight associated with the request, a number of requests of a particular type processed by the arbiter, or any combination thereof.

Abstract: Methods are provided to determine the entire genomic region of a particular HLA locus including both intron and exons. The resultant consensus sequences provides linkage information between different exons, and produces the unique sequence from each of the two genes from the individual sample being typed. The sequence information in intron regions along with the exon sequences provides an accurate HLA haplotype.

Abstract: Disclosed is a method for improving the sensitivity and accuracy of quantitative detection of polynucleotides in a sample, such a clinical specimen, by a method that utilizes a two- or three-step process of tagging/labeling target molecules and adding an adapter sequence for adding a universal primer for efficient amplification of targets while decreasing target amplification bias. When combined with the step of statistically correcting for sequencing errors, the method can significantly increase the accuracy of quantitative detection of polynucleotides in a sample.

Abstract: This disclosure relates to arbitration of different types of requests to access a cache. Features of this disclosure can be implemented in a graphics processing unit (GPU). In one embodiment, an arbiter can receive requests from a color processor and a depth processor and determine which of the received requests has the highest priority. The request with the highest priority can then be provided to the cache. The priority can be configurable. The arbiter can determine priority, for example, based on whether a location in the cache associated with a request is available, a weight associated with the request, a number of requests of a particular type processed by the arbiter, or any combination thereof.

Abstract: Methods are provided to determine the entire genomic region of a particular HLA locus including both intron and exons. The resultant consensus sequences provides linkage information between different exons, and produces the unique sequence from each of the two genes from the individual sample being typed. The sequence information in intron regions along with the exon sequences provides an accurate HLA haplotype.

Abstract: The present disclosure generally pertains to a multiplex method for analyzing samples comprising using polynucleotide amplification to produce amplified products wherein one or more target sequences are tagged with a non-interfering, non-canceling target-specific polynucleotide identification tag, pyrosequencing the amplified products through the non-canceling target-specific polynucleotide identification tag sequence to detect the presence of one or more specific polynucleotide identification tags. The presence of a specific polynucleotide identification tag being correlated with the presence of a specific target sequence.

Abstract: The invention provides a bogie which comprises a bolster, two side frames and four constant contact dampers, two sides of two ends of the bolster are respectively provided with a wedge pocket; the four constant contact dampers are respectively arranged in four wedge pockets, and each of the four constant contact dampers comprises a wedge and a damping spring; the damping spring is arranged in an inner cavity of the wedge; one end of the damping spring bears against a top inner surface of the inner cavity of the wedge, and the other end of the damping spring bears against a bottom plate of the wedge pocket, wherein the bogie further comprises four through holes and four ejectors which respectively correspond to the four constant contact dampers; the through holes are arranged on the bottom plate, and the position of each of the four through holes on the bottom plate corresponds to the position where the damping spring bears against the bottom plate and a load spring of the bogie; and one end of the ejector pen

Abstract: The present invention discloses a freight car bogie and a freight car. The freight car bogie comprises two wheel sets, two side frames arranged at outer side of said wheel sets, and a bolster fixedly connected to center position of each of said two side frames, respectively. The freight car bogie further comprises a K-shaped supporting device, wherein said K-shaped supporting device passes through preset holes on two sides of an abdominal region of said bolster; a first upper end and a second upper end, as well as a first lower end and a second lower end of said K-shaped supporting device are elastically connected with said two side frames respectively; and said first upper end and second upper end, and said first lower end and second lower end are respectively symmetrical with respect to a center line of said bolster.

Abstract: The invention provides a bogie which comprises a bolster, two side frames and four constant contact dampers, two sides of two ends of the bolster are respectively provided with a wedge pocket; the four constant contact dampers are respectively arranged in four wedge pockets, and each of the four constant contact dampers comprises a wedge and a damping spring; the damping spring is arranged in an inner cavity of the wedge; one end of the damping spring bears against a top inner surface of the inner cavity of the wedge, and the other end of the damping spring bears against a bottom plate of the wedge pocket, wherein the bogie further comprises four through holes and four ejectors which respectively correspond to the four constant contact dampers; the through holes are arranged on the bottom plate, and the position of each of the four through holes on the bottom plate corresponds to the position where the damping spring bears against the bottom plate and a load spring of the bogie; and one end of the ejector pen

Abstract: The present invention discloses a freight car bogie and a freight car. The freight car bogie comprises two wheel sets, two side frames arranged at outer side of said wheel sets, and a bolster fixedly connected to center position of each of said two side frames, respectively. The freight car bogie further comprises a K-shaped supporting device, wherein said K-shaped supporting device passes through preset holes on two sides of an abdominal region of said bolster; a first upper end and a second upper end, as well as a first lower end and a second lower end of said K-shaped supporting device are elastically connected with said two side frames respectively; and said first upper end and second upper end, and said first lower end and second lower end are respectively symmetrical with respect to a centre line of said bolster.