Patents by Inventor Chunming Ding
Chunming Ding has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11898208Abstract: The present invention concerns a method for the detection or monitoring of cancer using a biological sample selected from blood, plasma, serum, saliva, urine from an individual, said method comprising: (a) obtaining DNA from the said biological sample; (b) digesting the DNA sample with one or more methylation-sensitive restriction enzymes; (c) quantifying or detecting a DNA sequence of interest after step (b), wherein the target sequence of interest contains at least two methylation-sensitive restriction enzyme recognition sites; and (d) comparing the level of the DNA sequence from the individual to a normal standard, to detect, prognosticate or monitor cancer.Type: GrantFiled: August 29, 2019Date of Patent: February 13, 2024Assignee: The Chinese University of Hong KongInventors: Yuk-Ming Dennis Lo, Kwan Chee Allen Chan, Chunming Ding
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Patent number: 11597977Abstract: The present invention relates to new methods for diagnosing a pregnancy-associated disorder by analyzing fetal DNA present in the mother's blood. More specifically, this invention relies on the discovery that the maspin gene is differentially methylated in fetal DNA and in maternal DNA and provides these new diagnostic methods, which distinguish fetal DNA from maternal DNA and detect prenatal disorders based on abnormalities in fetal DNA level and methylation status.Type: GrantFiled: March 2, 2020Date of Patent: March 7, 2023Assignee: The Chinese University of Hong KongInventors: Yuk Ming Dennis Lo, Wai Kwun Rossa Chiu, Stephen Siu Chung Chim, Yu-Kwan Tong, Chunming Ding
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Publication number: 20200270694Abstract: The present invention relates to new methods for diagnosing a pregnancy-associated disorder by analyzing fetal DNA present in the mother's blood. More specifically, this invention relies on the discovery that the maspin gene is differentially methylated in fetal DNA and in maternal DNA and provides these new diagnostic methods, which distinguish fetal DNA from maternal DNA and detect prenatal disorders based on abnormalities in fetal DNA level and methylation status.Type: ApplicationFiled: March 2, 2020Publication date: August 27, 2020Inventors: YUK MING DENNIS LO, Wai Kwun Rossa Chiu, Stephen Siu Chung Chim, Yu-Kwan Tong, Chunming Ding
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Patent number: 10604808Abstract: The present invention relates to new methods for diagnosing a pregnancy-associated disorder by analyzing fetal DNA present in the mother's blood. More specifically, this invention relies on the discovery that the maspin gene is differentially methylated in fetal DNA and in maternal DNA and provides these new diagnostic methods, which distinguish fetal DNA from maternal DNA and detect prenatal disorders based on abnormalities in fetal DNA level and methylation status.Type: GrantFiled: December 14, 2017Date of Patent: March 31, 2020Assignee: The Chinese University of Hong KongInventors: Yuk Ming Dennis Lo, Rossa Wai Kwun Chiu, Stephen Siu Chung Chim, Yu-Kwan Tong, Chunming Ding
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Publication number: 20200071771Abstract: The present invention concerns a method for the detection or monitoring of cancer using a biological sample selected from blood, plasma, serum, saliva, urine from an individual, said method comprising: (a) obtaining DNA from the said biological sample; (b) digesting the DNA sample with one or more methylation-sensitive restriction enzymes; (c) quantifying or detecting a DNA sequence of interest after step (b), wherein the target sequence of interest contains at least two methylation-sensitive restriction enzyme recognition sites; and (d) comparing the level of the DNA sequence from the individual to a normal standard, to detect, prognosticate or monitor cancer.Type: ApplicationFiled: August 29, 2019Publication date: March 5, 2020Inventors: Yuk-Ming Dennis LO, Kwan Chee Allen CHAN, Chunming DING
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Patent number: 10435754Abstract: The present invention concerns a method for the detection or monitoring of cancer using a biological sample selected from blood, plasma, serum, saliva, urine from an individual, said method comprising: (a) obtaining DNA from the said biological sample; (b) digesting the DNA sample with one or more methylation-sensitive restriction enzymes; (c) quantifying or detecting a DNA sequence of interest after step (b), wherein the target sequence of interest contains at least two methylation-sensitive restriction enzyme recognition sites; and (d) comparing the level of the DNA sequence from the individual to a normal standard, to detect, prognosticate or monitor cancer.Type: GrantFiled: May 23, 2016Date of Patent: October 8, 2019Assignee: The Chinese University Of Hong KongInventors: Yuk Ming Dennis Lo, Kwan Chee Allen Chan, Chunming Ding
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Patent number: 10329606Abstract: Disclosed herein are methods and kits for selectively amplifying, detecting or quantifying a DNA fragment with a specific end sequence, especially generated following restriction enzyme digestion. This method can be used, for example, to detect a hypomethylated DNA fragment. This methods and kits are especially useful in detecting or quantifying a hypomethylated fetal DNA fragment in a maternal plasma sample containing a corresponding hypermethylated maternal DNA fragment.Type: GrantFiled: April 29, 2014Date of Patent: June 25, 2019Assignee: The Chinese University of Hong KongInventors: Yuk Ming Dennis Lo, Yu Kwan Tong, Wai Kwun Rossa Chiu, Chunming Ding
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Publication number: 20180155791Abstract: The present invention relates to new methods for diagnosing a pregnancy-associated disorder by analyzing fetal DNA present in the mother's blood. More specifically, this invention relies on the discovery that the maspin gene is differentially methylated in fetal DNA and in maternal DNA and provides these new diagnostic methods, which distinguish fetal DNA from maternal DNA and detect prenatal disorders based on abnormalities in fetal DNA level and methylation status.Type: ApplicationFiled: December 14, 2017Publication date: June 7, 2018Inventors: Yuk Ming Dennis LO, Rossa Wai Kwun Chiu, Stephen Siu Chung Chim, Yu-Kwan Tong, Chunming Ding
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Patent number: 9862999Abstract: The present invention relates to new methods for diagnosing a pregnancy-associated disorder by analyzing fetal DNA present in the mother's blood. More specifically, this invention relies on the discovery that the maspin gene is differentially methylated in fetal DNA and in maternal DNA and provides these new diagnostic methods, which distinguish fetal DNA from maternal DNA and detect prenatal disorders based on abnormalities in fetal DNA level and methylation status.Type: GrantFiled: February 1, 2016Date of Patent: January 9, 2018Assignee: The Chinese University of Hong KongInventors: Yuk Ming Dennis Lo, Wai Kwun Rossa Chiu, Stephen Siu-Chung Chim, Yu-Kwan Tong, Chunming Ding
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Publication number: 20170101685Abstract: The present invention concerns a method for the detection or monitoring of cancer using a biological sample selected from blood, plasma, serum, saliva, urine from an individual, said method comprising: (a) obtaining DNA from the said biological sample; (b) digesting the DNA sample with one or more methylation-sensitive restriction enzymes; (c) quantifying or detecting a DNA sequence of interest after step (b), wherein the target sequence of interest contains at least two methylation-sensitive restriction enzyme recognition sites; and (d) comparing the level of the DNA sequence from the individual to a normal standard, to detect, prognosticate or monitor cancer.Type: ApplicationFiled: May 23, 2016Publication date: April 13, 2017Inventors: YUK MING DENNIS LO, Kwan Chee Allen Chan, Chunming Ding
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Publication number: 20160258018Abstract: The present invention relates to new methods for diagnosing a pregnancy-associated disorder by analyzing fetal DNA present in the mother's blood. More specifically, this invention relies on the discovery that the maspin gene is differentially methylated in fetal DNA and in maternal DNA and provides these new diagnostic methods, which distinguish fetal DNA from maternal DNA and detect prenatal disorders based on abnormalities in fetal DNA level and methylation status.Type: ApplicationFiled: February 1, 2016Publication date: September 8, 2016Inventors: Yuk Ming Dennis Lo, Wai Kwun Rossa Chiu, Stephen Siu-Chung Chim, Yu-Kwan Tong, Chunming Ding
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Patent number: 9376714Abstract: The present invention is directed to a method for detecting and quantifying rare mutations in a nucleic acid sample. The nucleic acid molecules under investigation can be either DNA or RNA. The rare mutation can be any type of functional or non-functional nucleic acid change or mutation, such as deletion, insertion, translocation, inversion, one or more base substitution or polymorphism. Therefore, the methods of the present invention are useful in detection of rare mutations in, for example, diagnostic, prognostic and follow-up applications, when the targets are rare known nucleic acid variants mixed in with the wildtype or the more common nucleic acid variant(s).Type: GrantFiled: March 6, 2012Date of Patent: June 28, 2016Assignee: Trustees of Boston UniversityInventors: Charles R Cantor, Chunming Ding
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Patent number: 9371566Abstract: The present invention concerns a method for the detection or monitoring of cancer using a biological sample selected from blood, plasma, serum, saliva, urine from an individual, said method comprising: (a) obtaining DNA from the said biological sample; (b) digesting the DNA sample with one or more methylation-sensitive restriction enzymes; (c) quantifying or detecting a DNA sequence of interest after step (b), wherein the target sequence of interest contains at least two methylation-sensitive restriction enzyme recognition sites; and (d) comparing the level of the DNA sequence from the individual to a normal standard, to detect, prognosticate or monitor cancer.Type: GrantFiled: September 26, 2007Date of Patent: June 21, 2016Assignee: The Chinese University of Hong KongInventors: Yuk Ming Dennis Lo, Kwan Chee Allen Chan, Chunming Ding
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Publication number: 20150275300Abstract: Disclosed is an isolated biomarker/biomarker region. Also disclosed are isolated biomarker/biomarker regions for detecting trisomy 21, methods of determining the likelihood of a foetus to suffer from a specific disease using the biomarker/biomarker region, a kit and a method of determining the methylation levels of a biomarker/biomarker region.Type: ApplicationFiled: September 26, 2013Publication date: October 1, 2015Applicant: Agency for Science, Technology and ResearchInventors: Chunming Ding, Shengnan Jin, Yew Kok Lee, Seow Heong Yeo
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Patent number: 8927216Abstract: This application describes the discovery that, in a pregnant woman, certain genes (such as RASSF1A, APC, CASP8, RARB, SCGB3A1, DAB2IP, PTPN6, THY1, TMEFF2, and PYCARD) originated from a fetus are highly methylated, whereas the same genes of maternal origin are unmethylated. This discovery allows the easy detection of one or more of these methylated fetal genes in a biological sample from a pregnant woman, serving as a universal indicator of the presence of fetal DNA in the sample. These fetal methylation markers are particularly useful as positive controls for a non-invasive analytical process during which the quality and quantity of fetal DNA are monitored. These newly identified fetal markers can also be measured directly for diagnosis of certain pregnancy-related conditions.Type: GrantFiled: September 14, 2012Date of Patent: January 6, 2015Assignee: The Chinese University of Hong KongInventors: Yuk-Ming Dennis Lo, Rossa Wai Kwun Chiu, Stephen Siu Chung Chim, Chunming Ding, Kwan Chee Chan, Hing Nam Ivy Wong, Ka Chun Ryan Yuen
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Publication number: 20140302506Abstract: Disclosed herein are methods and kits for selectively amplifying, detecting or quantifying a DNA fragment with a specific end sequence, especially generated following restriction enzyme digestion. This method can be used, for example, to detect a hypomethylated DNA fragment. This methods and kits are especially useful in detecting or quantifying a hypomethylated fetal DNA fragment in a maternal plasma sample containing a corresponding hypermethylated maternal DNA fragment.Type: ApplicationFiled: April 29, 2014Publication date: October 9, 2014Applicant: The Chinese University of Hong KongInventors: Yuk Ming Dennis Lo, Yu Kwan Tong, Wai Kwun Rossa Chiu, Chunming Ding
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Publication number: 20140272975Abstract: The present invention concerns a method for the detection or monitoring of cancer using a biological sample selected from blood, plasma, serum, saliva, urine from an individual, said method comprising: (a) obtaining DNA from the said biological sample; (b) digesting the DNA sample with one or more methylation-sensitive restriction enzymes; (c) quantifying or detecting a DNA sequence of interest after step (b), wherein the target sequence of interest contains at least two methylation-sensitive restriction enzyme recognition sites; and (d) comparing the level of the DNA sequence from the individual to a normal standard, to detect, prognosticate or monitor cancer.Type: ApplicationFiled: May 22, 2014Publication date: September 18, 2014Applicant: THE CHINESE UNIVERSITY OF HONG KONGInventors: Yuk Ming Dennis LO, Kwan Chee Allen CHAN, Chunming DING
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Patent number: 8748100Abstract: Disclosed herein are methods and kits for selectively amplifying, detecting or quantifying a DNA fragment with a specific end sequence, especially generated following restriction enzyme digestion. This method can be used, for example, to detect a hypomethylated DNA fragment. This methods and kits are especially useful in detecting or quantifying a hypomethylated fetal DNA fragment in a maternal plasma sample containing a corresponding hypermethylated maternal DNA fragment.Type: GrantFiled: August 30, 2007Date of Patent: June 10, 2014Assignee: The Chinese University of Hong KongInventors: Yuk Ming Dennis Lo, Yu Kwan Tong, Wai Kwun Rossa Chiu, Chunming Ding
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Patent number: 8501443Abstract: The present invention provides an efficient way for high throughput haplotype analysis. Several polymorphic nucleic acid markers, such as SNPs, can be simultaneously and reliably determined through multiplex PCR of single nucleic acid molecules in several parallel single molecule dilutions and the consequent statistical analysis of the results from these parallel single molecule multiplex PCR reactions results in reliable determination of haplotypes present in the subject. The nucleic acid markers can be of any distance to each other on the chromosome. In addition, an approach wherein overlapping DNA markers are analyzed can be used to link smaller haplotypes into larger haplotypes. Consequently, the invention provides a powerful new tool for diagnostic haplotyping and identifying novel haplotypes.Type: GrantFiled: August 2, 2012Date of Patent: August 6, 2013Assignee: Trustees of Boston UniversityInventors: Charles R Cantor, Chunming Ding
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Patent number: 8435764Abstract: Disclosed is a method for the detection of virus variations. Disclosed also is a method for the treatment of virus infection in a subject based on the detection of virus variations. Additionally, a kit is provided for the detection of virus variations.Type: GrantFiled: May 4, 2010Date of Patent: May 7, 2013Assignees: The Chinese University of HongKong, The Third People's Hospital of ShenzhenInventors: Chunming Ding, Ju Luan, Boping Zhou, Jing Yuan, Xinchun Chen, Hongmei Zhang