Patents by Inventor Chunnian Shi
Chunnian Shi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8951719Abstract: The present invention provides compositions useful for biomolecule storage comprising a water soluble inorganic compound, a stabilizer, or a combination thereof. The present invention also provides methods of using the compositions of the invention to store biomolecules in the dry state and in solution, as well as sample carriers and kits comprising compositions of the invention.Type: GrantFiled: August 20, 2012Date of Patent: February 10, 2015Assignee: Gentegra, LLC.Inventors: Michael Hogan, Michael Saghbini, David Wong, Chunnian Shi
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Publication number: 20140227686Abstract: The present invention provides compositions and methods that enable the stabilization and storage of samples by contacting a sample with an assembly of particles, and reducing the water activity level of the contacted sample. By reducing the water activity level of the sample, the assembly of particles minimizes the degradation of the sample. Stabilizers may or may not be added to the assembly of particles to further minimize the degradation of the sample. Subsequently to storage in the assembly of particles, the samples are recoverable by eluting the assembly of particles with a fluid solution. In one embodiment, the entire assembly of particles will dissolve into the solution. In another embodiment, only part of the assembly of particles will dissolve into the solution. The assembly of particles provides the advantage that while it is porous, it comprises non-porous particulate material.Type: ApplicationFiled: April 16, 2014Publication date: August 14, 2014Applicant: GENTEGRA LLCInventors: Michael Saghbini, Michael Hogan, Chunnian Shi, Brian Dalby, David Wong
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Publication number: 20120308987Abstract: The present invention provides compositions useful for biomolecule storage comprising a water soluble inorganic compound, a stabilizer, or a combination thereof. The present invention also provides methods of using the compositions of the invention to store biomolecules in the dry state and in solution, as well as sample carriers and kits comprising compositions of the invention.Type: ApplicationFiled: August 20, 2012Publication date: December 6, 2012Applicant: GenVault CorporationInventors: Michael HOGAN, Michael SAGHBINI, Chunnian SHI, David WONG
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Patent number: 8283165Abstract: The present invention provides compositions useful for biomolecule storage comprising a water soluble inorganic compound, a stabilizer, or a combination thereof. The present invention also provides methods of using the compositions of the invention to store biomolecules in the dry state and in solution, as well as sample carriers and kits comprising compositions of the invention.Type: GrantFiled: September 14, 2009Date of Patent: October 9, 2012Assignee: GenVault CorporationInventors: Michael Hogan, Michael Saghbini, Chunnian Shi, David Wong
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Publication number: 20120100522Abstract: The present invention provides compositions and methods that enable the stabilization and storage of samples by contacting a sample with an assembly of particles, and reducing the water activity level of the contacted sample. By reducing the water activity level of the sample, the assembly of particles minimizes the degradation of the sample. Stabilizers may or may not be added to the assembly of particles to further minimize the degradation of the sample. Subsequently to storage in the assembly of particles, the samples are recoverable by eluting the assembly of particles with a fluid solution. In one embodiment, the entire assembly of particles will dissolve into the solution. In another embodiment, only part of the assembly of particles will dissolve into the solution. The assembly of particles provides the advantage that while it is porous, it comprises non-porous particulate material.Type: ApplicationFiled: April 6, 2011Publication date: April 26, 2012Applicant: GenVault CorporationInventors: Michael Saghbini, Michael Hogan, Chunnian Shi, Brian Dalby, David Wong
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Patent number: 7879554Abstract: The present disclosure provides methods and compositions for conducting an assay to detect a polynucleotide. In particular, ruthenium complexes having reduction potentials that do not coincide with the reduction potential of molecular oxygen are disclosed and amperometric techniques for their use are described. In preferred embodiments, the ruthenium complex is ruthenium (III) pentaamine pyridine and the polynucleotide that is detected is DNA. Further, techniques for enhancing detectable contrast between hybridized and unhybridized nucleic acids are disclosed. In particular, the use of elongated target strands as well as the use of uncharged probe strands are discussed.Type: GrantFiled: September 29, 2009Date of Patent: February 1, 2011Assignee: Geneohm Sciences, Inc.Inventors: Donald M. Crothers, R. Erik Holmlin, Honghua Zhang, Chunnian Shi
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Patent number: 7820389Abstract: Provided herein are compositions and methods for enhancing the relative efficiency of hybridization between target nucleic acids and capture probes compared to target variants and capture probes.Type: GrantFiled: January 4, 2008Date of Patent: October 26, 2010Assignee: Geneohm Sciences, Inc.Inventor: Chunnian Shi
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Publication number: 20100248363Abstract: The present invention provides compositions useful for biomolecule storage comprising a water soluble inorganic compound, a stabilizer, or a combination thereof. The present invention also provides methods of using the compositions of the invention to store biomolecules in the dry state and in solution, as well as sample carriers and kits comprising compositions of the invention.Type: ApplicationFiled: September 14, 2009Publication date: September 30, 2010Applicant: GENVAULT CORPORATIONInventors: Michael Hogan, Michael Saghbini, David Wong, Chunnian Shi
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Publication number: 20100167376Abstract: The present invention provides methods useful for making lysates from cell wall-containing cellular samples, including plant tissue samples and cultures of yeast or bacteria. The invention further provides compositions (e.g., solutions) that can be used in the methods of the invention, and kits comprising solutions and/or other reagents useful for carrying out the methods of the invention.Type: ApplicationFiled: October 6, 2009Publication date: July 1, 2010Applicant: GenVault CorporationInventors: Michael Hogan, Michael Saghbini, Joseph Utermohlen, Chunnian Shi
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Publication number: 20100018876Abstract: The present disclosure provides methods and compositions for conducting an assay to detect a polynucleotide. In particular, ruthenium complexes having reduction potentials that do not coincide with the reduction potential of molecular oxygen are disclosed and amperometric techniques for their use are described. In preferred embodiments, the ruthenium complex is ruthenium (III) pentaamine pyridine and the polynucleotide that is detected is DNA. Further, techniques for enhancing detectable contrast between hybridized and unhybridized nucleic acids are disclosed. In particular, the use of elongated target strands as well as the use of uncharged probe strands are discussed.Type: ApplicationFiled: September 29, 2009Publication date: January 28, 2010Applicant: GeneOhm SciencesInventors: Donald M. Crothers, R. Erik Holmlin, Honghua Zhang, Chunnian Shi
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Patent number: 7615348Abstract: The present disclosure provides methods and compositions for conducting an assay to detect a polynucleotide. In particular, ruthenium complexes having reduction potentials that do not coincide with the reduction potential of molecular oxygen are disclosed and amperometric techniques for their use are described. In preferred embodiments, the ruthenium complex is ruthenium (III) pentaamine pyridine and the polynucleotide that is detected is DNA. Further, techniques for enhancing detectable contrast between hybridized and unhybridized nucleic acids are disclosed. In particular, the use of elongated target strands as well as the use of uncharged probe strands are discussed.Type: GrantFiled: August 8, 2007Date of Patent: November 10, 2009Assignee: Genohm SciencesInventors: Donald M. Crothers, R. Erik Holmlin, Honghua Zhang, Chunnian Shi
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Publication number: 20080188005Abstract: Provided herein are compositions and methods for enhancing the relative efficiency of hybridization between target nucleic acids and capture probes compared to target variants and capture probes.Type: ApplicationFiled: January 4, 2008Publication date: August 7, 2008Inventor: Chunnian Shi
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Publication number: 20080026397Abstract: The present disclosure provides methods and compositions for conducting an assay to detect a polynucleotide. In particular, ruthenium complexes having reduction potentials that do not coincide with the reduction potential of molecular oxygen are disclosed and amperometric techniques for their use are described. In preferred embodiments, the ruthenium complex is ruthenium (III) pentaamine pyridine and the polynucleotide that is detected is DNA. Further, techniques for enhancing detectable contrast between hybridized and unhybridized nucleic acids are disclosed. In particular, the use of elongated target strands as well as the use of uncharged probe strands are discussed.Type: ApplicationFiled: August 8, 2007Publication date: January 31, 2008Applicant: GeneOhm SciencesInventors: Donald Crothers, R. Holmlin, Honghua Zhang, Chunnian Shi
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Patent number: 7258978Abstract: The present disclosure provides methods and compositions for conducting an assay to detect nucleic acid hybridization in the presence of oxygen. In particular, ruthenium complexes having a reduction potential that does not coincide with the reduction potential of molecular oxygen are disclosed and amperometric techniques for their use are described. In preferred embodiments, the ruthenium complex is ruthenium (III) pentaamine pyridine and the nucleic acid hybridization event that is detected is DNA hybridization. Further, techniques for enhancing detectable contrast between hybridized and unhybridized nucleic acids are disclosed. In particular, the use of elongated target strands as well as the use of uncharged probe strands are discussed.Type: GrantFiled: May 2, 2003Date of Patent: August 21, 2007Assignee: Geneohm SciencesInventors: Donald M. Crothers, R. Erik Holmlin, Honghua Zhang, Chunnian Shi
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Publication number: 20050186590Abstract: The present disclosure relates to methods of detecting nucleic acid hybridization by monitoring an output signal. Some advantageous embodiments include techniques which can augment the signal. One such technique involves catalytic detection, such as with a peroxidase or other enzyme. Another technique involves “on-chip” amplification to enlarge the nucleic acid after hybridization has occurred.Type: ApplicationFiled: November 10, 2004Publication date: August 25, 2005Inventors: Donald Crothers, R. Holmlin, Honghua Zhang, Chunnian Shi
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Publication number: 20050118616Abstract: The present disclosure relates to methods for amplifying nucleic acids and methods for detecting the amplified nucleic acid products. Nucleic acid amplification and detection can occur in solution or on a solid support.Type: ApplicationFiled: August 9, 2004Publication date: June 2, 2005Inventors: Tadashi Kawashima, Erik Holmlin, Donald Crothers, Honghua Zhang, Chunnian Shi
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Publication number: 20040086895Abstract: The present disclosure relates to the detection of somatic cell mutations, particularly as part of a method to screen for cancer or precancer. The disclosure includes techniques for extracting and isolating oligonucleotides from a patient and conducting hybridization assays. Preferred embodiments include a combination of the following steps: extracting a biological sample from a patient, purifying a nucleic acid from a biological sample, amplifying a nucleic acid, isolating a nucleic acid in single stranded form, cyclizing a nucleic acid, elongating a nucleic acid, controlling hybridization stringency, amplifying a nucleic acid on a chip, and detecting hybridization.Type: ApplicationFiled: May 2, 2003Publication date: May 6, 2004Inventors: Donald M. Crothers, R. Erik Holmlin, Chunnian Shi
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Publication number: 20040086894Abstract: The present disclosure provides methods and compositions for conducting an assay to detect nucleic acid hybridization in the presence of oxygen. In particular, ruthenium complexes having a reduction potential that does not coincide with the reduction potential of molecular oxygen are disclosed and amperometric techniques for their use are described. In preferred embodiments, the ruthenium complex is ruthenium (III) pentaamine pyridine and the nucleic acid hybridization event that is detected is DNA hybridization. Further, techniques for enhancing detectable contrast between hybridized and unhybridized nucleic acids are disclosed. In particular, the use of elongated target strands as well as the use of uncharged probe strands are discussed.Type: ApplicationFiled: May 2, 2003Publication date: May 6, 2004Inventors: Donald M. Crothers, R. Erik Holmlin, Honghua Zhang, Chunnian Shi