Abstract: A complex containing undenatured type II collagen and chitosan oligosaccharide, and a preparation method therefor. The preparation method comprises the following steps: (1) activation: mixing a certain amount of powder or particulate matter containing undenatured type II collagen with an activating agent and storing the mixture at 0° C. to 80° C. for 1 min to 300 min; (2) sieving: sieving the mixture obtained in step (1) to remove unreacted activating agent; (3) coupling: adding chitosan oligosaccharide to the mixture obtained in step (2) in which the unreacted activating agent is removed, and dynamically mixing them at 0° C. to 80° C. for 1 min to 300 min; and (4) drying: drying the mixture obtained in step (3) and controlling the water content to be 8% or less, the resulting product being a complex containing undenatured type II collagen and chitosan oligosaccharide. This preparation method is simple to implement, with fewer operation steps and relatively high efficiency.

Abstract: Disclosed in the present invention are a preparation method for and a use method of a collagen microfiber hemostatic material. Collagen microfibers are obtained by performing high-speed shearing on a solid collagen material, after the collagen microfibers are dispersed in an aqueous phase, a procoagulant active ingredient modifies the surfaces of the collagen microfibers by means of linking molecules; and a collagen microfiber hemostatic material is obtained by performing high-speed shearing again after freeze drying. The collagen microfiber material can be prepared simply and efficiently by means of high-speed shearing; the biological activity of the collagen material is maintained as much as possible; and the microfibers have a length range of 10-1000 ?m and high dispersibility, thus facilitating spray processing.