Abstract: Systems and methods relating thereto for detecting target analytes, using hand-held detection devices and compression mechanisms, are described. An exemplar hand-held detection device includes a cartridge inlet and a cartridge stage for securing cartridge containing the sample including the target-analyte. The device further comprises a compression assembly including a pressing surface. In an open state of the compression assembly, the pressing surface is released, displacing away from the cartridge stage, and thereby allowing a cartridge inlet to provide access to an unobstructed loading path for the cartridge to be secured on the cartridge stage. Upon receiving an external pressing force on the pressing surface, the compression assembly is designed to acquire a compressed state, in which the pressing surface displaces towards the cartridge stage and the compression assembly seals off the cartridge present inside the cartridge stage from an environment around the cartridge stage.

Abstract: A cartridge for providing a target analyte for detection is described. One such exemplar cartridge includes a base portion including: (1) a receiving area disposed at or near a center region of the base portion; (2) multiple reaction wells disposed outside the center region or radially disposed at or near a perimeter of the base portion; and (3) multiple connecting tracks that substantially linearly extend from a region at or proximate to the receiving area to the multiple reaction wells and designed to convey a sample including the target analyte from the receiving area to the multiple reaction wells, each of which are configured to transform the sample to a detectable sample. Systems and methods of reacting and detecting the sample including the target analyte are also described.

Abstract: A cartridge for providing a target analyte for detection is described. One such exemplar cartridge includes a base portion including: (1) a receiving area disposed at or near a center region of the base portion; (2) multiple reaction wells disposed outside the center region or radially disposed at or near a perimeter of the base portion; and (3) multiple connecting tracks that substantially linearly extend from a region at or proximate to the receiving area to the multiple reaction wells and designed to convey a sample including the target analyte from the receiving area to the multiple reaction wells, each of which are configured to transform the sample to a detectable sample. Systems and methods of reacting and detecting the sample including the target analyte are also described.

Abstract: A cartridge for providing a target analyte for detection is described. One such exemplar cartridge includes a base portion including: (1) a receiving area disposed at or near a center region of the base portion; (2) multiple reaction wells disposed outside the center region or radially disposed at or near a perimeter of the base portion; and (3) multiple connecting tracks that substantially linearly extend from a region at or proximate to the receiving area to the multiple reaction wells and designed to convey a sample including the target analyte from the receiving area to the multiple reaction wells, each of which are configured to transform the sample to a detectable sample. Systems and methods of reacting and detecting the sample including the target analyte are also described.

Abstract: Disclosed herein are pressure control systems, comprising: a touch screen electrically connected to industrial computer or embedded operating system; a pressure output channel connected to the touch screen and/or the industrial computer or embedded operating system; and pressure control unit for communicating with the pressure output channel, wherein the pressure control unit can be controlled with the touch screen, and wherein the industrial computer or embedded operating system comprises preinstalled software with User Interface (UI) for pressure control setup and running the system.

Abstract: Disclosed herein are microfluidic devices and systems for amplifying and detecting a target polynucleotide, comprising: one or more wells for receiving one or more substrates; a droplet generation channel in fluid communication with the one or more wells, wherein the microfluidic channel is adapted to generate droplets; and a chamber in fluid communication with the droplet generation channel, and adapted to collect droplets generated by the droplet generation channel, and further adapted to perform nucleic acid amplification in droplets, and further adapted to detect light signal from droplets. Also disclosed are methods of using the same.

Abstract: Disclosed herein are systems comprising a pressure adjustment manifold, a pressure distribution manifold, and an electronic board, each of which are operatively connected. Also provided herein are methods of making and using the same.

Abstract: Disclosed herein are microfluidic devices and systems for amplifying and detecting a target polynucleotide, comprising: one or more wells for receiving one or more substrates; a droplet generation channel in fluid communication with the one or more wells, wherein the microfluidic channel is adapted to generate droplets; and a chamber in fluid communication with the droplet generation channel, and adapted to collect droplets generated by the droplet generation channel, and further adapted to perform nucleic acid amplification in droplets, and further adapted to detect light signal from droplets. Also disclosed are methods of using the same.

Abstract: Disclosed herein are microfluidic devices comprising, one or more inlets in flow communication with one or more microfluidic channels, wherein the one or more inlets are adapted for receiving deformable beads, oil, and/or a suspension comprising buffer, cells, and/or particles, wherein the one or more microfluidic channels are in flow communication with the one or more inlets through a cross junction and define a fluid flow path therebetween, said fluid flow path forming a substantially planar substrate, and wherein the microfluidic channel is adapted to generate droplets. Also disclosed are methods of making and using the same.

Abstract: Disclosed herein are pressure control systems, comprising: a touch screen electrically connected to industrial computer or embedded operating system; a pressure output channel connected to the touch screen and/or the industrial computer or embedded operating system; and pressure control unit for communicating with the pressure output channel, wherein the pressure control unit can be controlled with the touch screen, and wherein the industrial computer or embedded operating system comprises preinstalled software with User Interface (UI) for pressure control setup and running the system.

Abstract: Disclosed herein are systems comprising a pressure adjustment manifold, a pressure distribution manifold, and an electronic board, each of which are operatively connected. Also provided herein are methods of making and using the same.