Patents by Inventor Cindy Ast

Cindy Ast has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • BIOSENSORS AND METHODS OF USE
    Publication number: 20170247769
    Abstract: The present disclosure provides fluorescent polypeptides containing a fusion of a circularly permuted, first fluorescent protein and a second fluorescent protein, the first fluorescent protein containing a first fluorescent moiety and the second fluorescent protein containg a second fluorescent moiety, wherein the second fluorescent protein is contained in the circularly permuted first fluorescent protein to form a cassette that can be inserted into sites within a sensing protein of interest to form novel biosensors; alternatively, the reference domain can be inserted into an existing single-fluorescent protein-based, intensiometric biosensor in order to make a ratiometric biosensor in one cloning step; nucleic acid sequences encoding the fluorescent polypeptides, fluorescent sensors including the fluorescent polypeptides, and methods of making and using same are described herein.
    Type: Application
    Filed: February 21, 2017
    Publication date: August 31, 2017
    Inventors: Cindy AST, Luke M. OLTROGGE, Wolf B. FROMMER
  • TRANSMEMBRANE PROTEINS AS BIOSENSORS
    Publication number: 20160116457
    Abstract: The invention relates to engineered transporter proteins comprising at least one fluorescent reporter covalently bound to the transporter protein, wherein the transporter proteins of the present invention comprise a structural inverted repeat motif, with the motif comprising a first and second subunit that are structural inverted repeats of one another and that are joined to one another by a polypeptide loop.
    Type: Application
    Filed: November 2, 2015
    Publication date: April 28, 2016
    Inventors: Wolf B. Frommer, Roberto De Michele, Cindy Ast
  • Transmembrane protein as biosensors
    Patent number: 9176143
    Abstract: The disclosure relates to engineered transport proteins comprising at last one fluorescent reporter covalently bound to the transporter protein, wherein the transporter proteins comprise a structural inverted repeat motif, with the motif comprising a first and second subunit that are structural inverted repeats of one another and that are joined to one another by a polypeptide loop.
    Type: Grant
    Filed: September 18, 2012
    Date of Patent: November 3, 2015
    Assignee: Carnegie Institution of Washington
    Inventors: Wolf B. Frommer, Roberto De Michele, Cindy Ast
  • Transmembrane Protein as Biosensors
    Publication number: 20140356896
    Abstract: The disclosure relates to engineered transport proteins comprising at last one fluorescent reporter covalently bound to the transporter protein, wherein the transporter proteins comprise a structural inverted repeat motif, with the motif comprising a first and second subunit that are structural inverted repeats of one another and that are joined to one another by a polypeptide loop.
    Type: Application
    Filed: September 18, 2012
    Publication date: December 4, 2014
    Inventors: Wolf B. Frommer, Roberto De Michele, Cindy Ast