Abstract: Provided herein are methods for the preparation of perfusable scaffolds for cell culture. These methods can comprise providing a bioink composition and a fugitive ink composition; chaotic printing the bioink composition and the fugitive ink composition to generate a microstructured precursor comprising a plurality of lamellar structures formed from the bioink composition; curing the bioink composition to form a cured scaffold precursor; and removing the fugitive ink from the cured scaffold precursor, thereby forming the perfusable scaffold. Also provided are scaffolds prepared by these methods as well as modular bioreactors incorporating these scaffolds.

Abstract: The present disclosure describes additive manufacturing methods, in particular methods for optimizing the manufacture of objects by three-dimensional printing processes. Also provided are bioreactors as well as methods of using thereof.

Abstract: Disclosed herein is an apparatus for fabricating a branching structure, the apparatus comprising: a flexible, electrically conductive internal electrical field collector comprising a first collector end, a second collector end, a collector outer surface located between both collector ends, a collector longitudinal axis extending through the first collector end and the second collector end, and at least one articulating feature positioned between the first collector end and the second collector end, a compression and rotation mechanism in contact with the first collector end, and a continuously formed mandrel that can include branches, having a first mandrel circumference with a mandrel inner circumference larger than the collector outer circumference and positionable over the internal electrical field collector and a second mandrel located at sufficient distance outside the first mandrel to facilitate attraction of electruspun fibers.