Abstract: A protocol process is provided for testing and evaluating Bacillus spores. The process includes isolating a sample of the spores, providing an environmental matrix of exposure condition elements, determining a midpoint for each control range between the low and high extremities, selecting condition elements from the matrix, with each condition element corresponding to a first condition, subjecting the sample to the condition elements from the matrix, counting survival spores for each condition element, performing a response surface methodology (RSM) analysis from the survival spores, and determining coefficients that satisfy a relation for k conditions. The matrix has control ranges of temperature, relative humidity and exposure duration, the control ranges extending from a low extremity to a high extremity. The first condition is one of the low and high range extremities and remaining conditions occupying the midpoint for a corresponding control range. All condition elements are uniquely distinguishable.