Patents by Inventor Clara Gonzalez-Marin
Clara Gonzalez-Marin has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 12221623Abstract: Methods for processing and sorting sperm are disclosed. Portions of sperm sorting or staining processes may include standardizing sperm samples by adjusting the concentration of the sperm sample to a predetermined concentration and adjusting the pH of the sample to a predetermined value. Sperm may also be stained in a single staining buffer having a DNA selective dye and a quenching dye.Type: GrantFiled: March 4, 2013Date of Patent: February 11, 2025Assignee: INGURAN, LLCInventors: Thomas Boyd Gilligan, Kenneth Michael Evans, Richard Lenz, Clara Gonzalez-Marin, Ramakrishnan Vishwanath
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Publication number: 20230303970Abstract: The invention encompasses methods for reducing the proportion of sperm cells with abnormal morphology, and unviable sperm cells, in a sperm cell population.Type: ApplicationFiled: April 13, 2023Publication date: September 28, 2023Applicant: Inguran, LLCInventors: Juan Moreno, Kenneth Michael Evans, Ramakrishnan Vishwanath, Clara Gonzalez-Marin
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Patent number: 11629330Abstract: The invention encompasses methods for reducing the proportion of sperm cells with abnormal morphology, and unviable sperm cells, in a sperm cell population.Type: GrantFiled: March 18, 2020Date of Patent: April 18, 2023Assignee: Inguran, LLCInventors: Juan Moreno, Kenneth Michael Evans, Ramakrishnan Vishwanath, Clara Gonzalez-Marin
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Patent number: 11448653Abstract: Cell sorting methods that improve sorting efficiency and productivity by elevating sorting pressures and incorporate certain steps to help the cells better survive such elevated pressures. In the case of sperm, sorting the steps of standardizing sperm samples, staining sperm samples in a single step, calibrating a flow cytometer to place sperm in the leading edge of droplets, and changing a catch fluid distance may be incorporated individually, or in combination to help sperm better survive the sex sorting process.Type: GrantFiled: March 2, 2020Date of Patent: September 20, 2022Assignee: Inguran, LLCInventors: Kenneth Michael Evans, Thomas Boyd Gilligan, Richard W. Lenz, Ramakrishnan Vishwanath, Clara Gonzalez-Marin
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Publication number: 20220186182Abstract: Embodiments of the present invention relate generally to processes, systems, and compositions useful in manipulating a ratio of viable X chromosome bearing sperm to viable Y chromosome bearing sperm in at least one sperm population and useful for preserving the resulting manipulated sperm population. In some embodiments a cryoprotectant may be incorporated into various medias used in manipulating the sperm sample, such as in a staining media, a sheath fluid, and a collection media.Type: ApplicationFiled: February 7, 2022Publication date: June 16, 2022Applicant: Inguran, LLCInventors: Thomas B. Gilligan, Clara Gonzalez-Marin, Ramakrishnan Vishwanath
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Patent number: 11279913Abstract: Embodiments of the present invention relate generally to processes, systems, and compositions useful in manipulating a ratio of viable X chromosome bearing sperm to viable Y chromosome bearing sperm in at least one sperm population and useful for preserving the resulting manipulated sperm population. In some embodiments a cryoprotectant may be incorporated into various medias used in manipulating the sperm sample, such as in a staining media, a sheath fluid, and a collection media.Type: GrantFiled: January 19, 2018Date of Patent: March 22, 2022Assignee: Inguran, LLCInventors: Thomas B. Gilligan, Clara Gonzalez-Marin, Ramakrishnan Vishwanath
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Publication number: 20200299640Abstract: The invention encompasses methods for reducing the proportion of sperm cells with abnormal morphology, and unviable sperm cells, in a sperm cell population.Type: ApplicationFiled: March 18, 2020Publication date: September 24, 2020Applicant: Inguran, LLCInventors: Juan Moreno, Kenneth Michael Evans, Ramakrishnan Vishwanath, Clara Gonzalez-Marin
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Publication number: 20200200762Abstract: Cell sorting methods that improve sorting efficiency and productivity by elevating sorting pressures and incorporate certain steps to help the cells better survive such elevated pressures. In the case of sperm, sorting the steps of standardizing sperm samples, staining sperm samples in a single step, calibrating a flow cytometer to place sperm in the leading edge of droplets, and changing a catch fluid distance may be incorporated individually, or in combination to help sperm better survive the sex sorting process.Type: ApplicationFiled: March 2, 2020Publication date: June 25, 2020Applicant: Inguran, LLCInventors: Kenneth Michael Evans, Thomas Boyd Gilligan, Richard W. Lenz, Ramakrishnan Vishwanath, Clara Gonzalez-Marin
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Patent number: 10620213Abstract: Cell sorting methods that improve sorting efficiency and productivity by elevating sorting pressures and incorporate certain steps to help the cells better survive such elevated pressures. In the case of sperm, sorting the steps of standardizing sperm samples, staining sperm samples in a single step, calibrating a flow cytometer to place sperm in the leading edge of droplets, and changing a catch fluid distance may be incorporated individually, or in combination to help sperm better survive the sex sorting process.Type: GrantFiled: October 3, 2013Date of Patent: April 14, 2020Assignee: Inguran, LLCInventors: Kenneth Michael Evans, Thomas Boyd Gilligan, Richard W. Lenz, Ramakrishnan Vishwanath, Clara Gonzalez-Marin
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Publication number: 20180208894Abstract: Embodiments of the present invention relate generally to processes, systems, and compositions useful in manipulating a ratio of viable X chromosome bearing sperm to viable Y chromosome bearing sperm in at least one sperm population and useful for preserving the resulting manipulated sperm population. In some embodiments a cryoprotectant may be incorporated into various medias used in manipulating the sperm sample, such as in a staining media, a sheath fluid, and a collection media.Type: ApplicationFiled: January 19, 2018Publication date: July 26, 2018Applicant: Inguran, LLCInventors: Thomas B. Gilligan, Clara Gonzalez-Marin, Ramakrishnan Vishwanath
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Patent number: 9581587Abstract: A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.Type: GrantFiled: September 28, 2012Date of Patent: February 28, 2017Assignee: Inguran, LLCInventors: Kenneth Michael Evans, Thomas B. Gilligan, Clara Gonzalez-Marin
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Publication number: 20160010057Abstract: Embodiments of the claimed invention relate to a method of producing sex sorted sperm having an improved dose response which includes the steps of extending a sperm sample with a buffering holding media and adjusting the concentration of the extended sperm sample to a target concentration range. The extended sperm may then be stained with a DNA selective dye and sex sorted with a flow cytometer into a catch fluid. During the extending and sorting of the sperm sample, the pH may be maintained at a target pH. Additionally, at least one of the buffering holding media, the DNA selective dye and the catch fluid may be supplemented with at least one antioxidant.Type: ApplicationFiled: September 22, 2015Publication date: January 14, 2016Applicant: INGURAN, LLCInventors: Ramakrishnan Vishwanath, Kenneth Michael Evans, Thomas B. Gilligan, Juan Moreno, Richard Lenz, Clara Gonzalez-Marin
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Patent number: 9140688Abstract: A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.Type: GrantFiled: September 28, 2012Date of Patent: September 22, 2015Assignee: Inguran, LLCInventors: Kenneth Michael Evans, Thomas B. Gilligan, Clara Gonzalez-Marin
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Publication number: 20150072340Abstract: A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.Type: ApplicationFiled: September 28, 2012Publication date: March 12, 2015Applicant: INGURAN, LLCInventors: Kenneth Michael Evans, Thomas B. Gilligan, Clara Gonzalez-Marin
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Publication number: 20140234833Abstract: A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.Type: ApplicationFiled: September 28, 2012Publication date: August 21, 2014Applicant: INGURAN, LLCInventors: Kenneth Michael Evans, Thomas B. Gilligan, Clara Gonzalez-Marin
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Publication number: 20140099627Abstract: Methods for processing and sorting sperm are disclosed. Portions of sperm sorting or staining processes may include standardizing sperm samples by adjusting the concentration of the sperm sample to a predetermined concentration and adjusting the pH of the sample to a predetermined value. Sperm may also be stained in a single staining buffer having a DNA selective dye and a quenching dye.Type: ApplicationFiled: March 4, 2013Publication date: April 10, 2014Applicant: INGURAN, LLCInventors: Thomas Boyd Gilligan, Kenneth Michael Evans, Richard Lenz, Clara Gonzalez-Marin, Ramakrishnan Vishwanath