Abstract: The invention provides for a novel human checkpoint kinase gene, hCDS1, comprising the nucleic acid sequence of residues 66-1694 of SEQ ID NO: 1, a protein expressed from the gene, fragments and functional equivalents of the protein. The invention also provides for pharmaceutical compositions comprising a hCDS1 protein, and methods utilizing the protein.

Abstract: The present invention encompasses novel mammalian cell cycle checkpoint genes/DNA repair genes, cDNA or genomic DNA, isolated nucleic acids corresponding thereto, proteins encoded thereby, expression vectors comprising said nucleic acids, host cells transformed with said expression vectors, and methods for treating a cell using such nucleic acids or proteins.

Abstract: The present invention encompasses novel mammalian cell cycle checkpoint genes/DNA repair genes, cDNA or genomic DNA, isolated nucleic acids corresponding thereto, expression vectors comprising said nucleic acids, host cells transformed with said expression vectors, pharmaceutical compositions and the formulation of such compositions comprising said nucleic acids or proteins expressed therefrom, methods for treating a cell using such nucleic acids, proteins or pharmaceutical compositions, and the use of such nucleic acids or proteins in formulating a pharmaceutical preparation.

Abstract: Isolated mammalian Mus81-Eme endonuclease complexes comprise an Mus81 protein portion and an Eme protein portion. A method of identifying a chemical compound that modulates mammalian cellular response to DNA damage comprises contacting a chemical compound to be tested with a biochemical mixture containing an isolated mammalian Mus81-Eme1 endonuclease complex, a source of magnesium ion, and a suitable DNA substrate; measuring the activity level of mammalian Mus81-Eme endonuclease complex in the mixture; comparing the measured activity level to the activity level of a substantially similar mixture of isolated Mus81-Eme1 endonuclease, magnesium ion, and DNA substrate in the absence of the chemical compound to be tested; and selecting a chemical compound that increases or decreases the endonuclease activity. Isolated mammalian Eme1 and Eme2 proteins derived from humans and murine species and isolated nucleic acids encoding the proteins are also described.

Abstract: The invention provides for a novel human checkpoint kinase gene, hCDS1, translated protein, compositions, methods, and kits.

Abstract: The invention provides for a novel human checkpoint kinase gene, hCDS1, comprising the nucleic acid sequence of residues 66-1694 of SEQ ID NO: 1, a protein expressed from the gene, fragments and functional equivalents of the protein. The invention also provides for pharmaceutical compositions comprising a hCDS1 protein, and methods utilizing the protein.

Abstract: The invention provides for a novel human checkpoint kinase gene, hCDS 1, translated protein, compositions, methods, and kits.