Abstract: An enriched fetal cell sample is prepared from maternal blood by first separating a maternal blood sample on a three layer density gradient, and collection of the layer containing the lymphocytes and a layer immediately beneath the lymphocytes which presumably contains the nucleated fetal red blood cells and low density maternal red blood cells. The top layer in the gradient would contain the plasma and lighter components and the bottom layer would contain high density maternal red blood cells. The collected cells are then placed in a hypotonic solution and centrifuged to separate a fetal cell pellet, which contains enriched levels of fetal cells of varying types. The recovered pellet is fixed twice with Carnoy fixative and the fixed cells are then spread on a slide for FISH analysis.

Abstract: A method and device for detecting myoglobin amongst other mammalian peroxidatively active substances like hemoglobin is disclosed. Since the existing peroxidase-sensitive components do not discriminate between myoglobin and hemoglobin, the present method and device provide discrimination by including a filtration step or component which separates myoglobin from hemoglobin on the basis of their difference in molecular weights. The present method and device offer a quick and easy detection of myoglobin, especially in urine, and may conveniently use existing reagents and apparatus.

Abstract: A multi-part oxy-fuel cutting tip has mixture outlet passages formed in the tip skirt to form grooves. The grooves then cooperate with the tip core to form the outlet passages when the skirt is placed over the tip core. Oppositely directed circumferential inlet passages for the preheat oxygen and fuel promote rapid mixing while producing a non-rotating mixture flow. In addition, the sectional areas of the outlet passages decrease in the flow direction. The tip core has an annular chamfer at its outlet end. These features promote the rapid creation of a laminar mixture flow.

Abstract: A multi-part oxy-fuel cutting tip has mixture outlet passages formed in the tip skirt to form grooves. The grooves then cooperate with the tip core to form the outlet passages when the skirt is placed over the tip core. Oppositely directed circumferential inlet passages for the preheat oxygen and fuel promote rapid mixing while producing a non-rotating mixture flow. In addition, the sectional areas of the outlet passages decrease in the flow direction. The tip core has an annular chamfer at its outlet end. These features promote the rapid creation of a laminar mixture flow.

Abstract: A multi-part oxy-fuel cutting tip has mixture outlet passages formed by swaging the tip skirt to form grooves. The grooves then cooperate with the tip core to form the outlet passages when the skirt is placed over the tip core. Oppositely directed circumferential inlet passages for the preheat oxygen and fuel promote rapid mixing while producing a non-rotating mixture flow. In addition, the sectional areas of the outlet passages decrease in the flow direction. These features promote the rapid creation of a laminar mixture flow.