Abstract: A device for assuring that restroom users are effectively reminded to wash and cleanse their hands. A spray pump is attached adjacent to an exterior door-handle to a restroom and is activated either by the turning of the handle or by the opening of the door. The spray pump includes a spray nozzle which sprays the user's front palm and/or back of the hand with a dye stain when the door-handle is opened. The dye stain is non-toxic fast drying and easily washable with cleanser such as soap and water. The dye stain can be visible in natural light and include colors such as red, yellow, blue and the like. Alternatively, the dye stain can be only visible to infrared light. The device can be used with round circular door-handles and lever door-handles. A shield around the door handle can discourage attempts to defeat the staining.

Abstract: The present invention describes methods for screening or selecting novel proteolytic enzymes. These enzymatic functions can be selected from isolated chromosomal DNA libraries or from pools of mutagenized DNA encoding a proteolytic function. In particular, the selection method of the present invention, a phage gene is chosen that encodes a gene product necessary for the production of a phage. The phage carrying the modified gene is introduced into a host. Also, DNA libraries present within a cloning vector are introduced into host cells. The host cells are grown under conditions where the introduced libraries are expressed in the host cells. The presence of an enzymatic function capable of cleaving the target peptide is identified on the basis of production of infective phage.

Abstract: The present invention describes methods of screening for or selecting a catalytic antibody effective to cleave a target peptide. In particular, in the selection method of the present invention a phage gene is selected that encodes a gene product necessary for the production of a phage. The phage, carrying the modified gene is introduced into a host. Also, a library of rearranged immunoglobulin genes in a cloning vector is introduced into host cells. The host cells are grown under conditions in which the immunoglobulin genes are expressed in the host cells. The presence of antibodies capable of cleaving the target peptide is identified on the basis of production of phage.