Abstract: Disclosed is a method for controlling the microbiological quality of an environmental aqueous medium, suspected of containing various micro-organisms, having the following steps: selecting a reference set, having at least three micro-organisms, representing jointly or separately a microbiological quality level; providing a microbiological detection kit, having at least three probes specifically and respectively identifying said three micro-organisms; after treating the medium to be analyzed, contacting said micro-organisms, or any fraction thereof derived from the medium to be analyzed therefrom, with said detection kit, whereby a multiple determination of the micro-organisms is carried out, the determination representing the microbiological quality level of the medium.

Abstract: Disclosed is a method for detecting or identifying the original animal species in a sample likely to contain an ingredient obtained at least from said species.

Abstract: A method for aqueous phase nucleic acid isolation from a sample, comprising a step of nucleic acid adsorption on a particulate substrate, is disclosed. The method comprises an adsorption reagent preparation step (a) that includes a sol consisting of a aqueous continuous phase and a dispersed particulate substrate phase including a functionalized particulate polymer prepared by polymerizing (1) a first water-soluble acrylamide or acrylamide derivative monomer, (2) at least one cross-linking agent and (3) at least one second water-soluble, cationic and functional monomer, said polymer having a predetermined lower critical solubility temperature (LCST) of 25-45° C.

Abstract: The invention concerns a method for controlling the microbiological quality of an environmental aqueous medium, suspected of containing various micro-organisms, comprising the following steps: selecting a reference set, consisting of at least three micro-organisms, representing jointly or separately, a microbiological quality level; providing a microbiological detection kit, consisting of at least three probes specifically and respectively identifying said three micro-organisms; after treating the medium to be analysed, contacting said micro-organisms, or any fraction thereof derived from the medium to be analysed therefrom, with said detection kit, whereby a multiple determination of said micro-organisms is carried out, said determination representing the microbiological quality level of the medium. The invention also concerns an appropriate microbiological detection kit for implementing said method.

Abstract: An apparatus including at least one sonotrode (2) designed to generate ultrasound of variable power in at least one biological sample (5) containing cells to be lysed, the sample (5) being contained in at least one receptacle (4 or 10) such that the sonotrode (2) is in direct contact with the receptacle(s) (4). Also disclosed is a method for using ultrasound to lyse a biological sample (5) contained in a receptacle (4), which includes placing the receptacle (4) in direct contact with the sonotrode (2), and activating the sonotrode (2) for long enough to lyse the cells in the sample (5) but preserve the DNA and/or RNA molecules released for subsequent operations, e.g. amplification. The invention is particularly applicable in the discipline of molecular biology.

Abstract: The invention concerns a single-stranded nucleotide fragment comprising at least a sequence of 12 contiguous nucleotide patterns, belonging to a 23S ribosome RNA strand of the species of the Chlamydia genus or to is complementary strand, said sequence being selected in any one of the following groups consisting of said RNA zones: Group I: 451-472, 542-570, 596-623, 731-756, 878-890, 996-1020, 1061-1094, 1123-1186, 1857-1880, 2234-2307, 2341-2370; Group II: 420-450, 473-514, 694-713, 756-790, 842-857, 927-937, 1231-1248, 1241-1319, 1371-1381, 1880-1895, 1943-1961, 2151-2182; Group III: 404-426, 436-457, 466-515, 683-722, 747-808, 817-863, 891-955, 1024-1055, 1208-1251, 1315-1350, 1407-1548, 1364-1388, 1576-1622, 1891-1918, 2148-218; the first number corresponding to the position of the first nucleotide of said zone relative to the 23S ribosome RNA nucleotide sequence of serotype A of Chlamydia trachomatis, SEQ ID NO:55, selected as reference sequence, and the second number corresponding to the position of the

Abstract: A single-stranded nucleotide fragment belonging to a variable region of the ribosomal RNA 23S of species of the genus mycobacterium. Probes and primers with sequences belonging to those of the single-stranded nucleotide fragments, a reagent and a method for identifying the mycobacterial species.

Abstract: The invention relates to probes and primers derived from the 16S ribosomal RNA of pathogenic species of the genus Corynebacteria. In addition, a method of detecting and/or identifying pathogenic species of the genus Corynebacteria utilizing the probes and primers is disclosed.

Abstract: The invention relates to oligonucleotide primers and probes that can specifically hybridize to the rDNA or rRNA of at least one species of the genus Rickettsia.

Abstract: Nucleotide fragment of DNA, whose nucleotide sequence is included in the gene of the species of the genus Mycobacterium, coding for the 65-kD mycobacterial antigen, containing regions which are homologous in practically all species of the genus Mycobacterium, and at least one species-specific variable region, characterized in that said fragment is chosen from fragments whose nucleotide sequences possess at least 70% homology, and preferably at least 85% homology, with a predetermined sequence or its complementary sequence, said predetermined sequence beginning at nucleotide 438 and ending at nucleotide 751 of said gene coding for said antigen of all species of mycobacteria except for the species M. tuberculosis, M. bovis BCG, M. avium, M. paratuberculosis, M. fortuitum, M. malmoense, M. leprae, M. kansaii and M. marinum.

Abstract: The invention relates to a single-stranded oligonucleotide chosen from oligonucleotides having a sequence of at least 12 consecutive nucleotide units which is included in one of the sequences SEQ ID NO: 1 to SEQ ID NO: 52, and from the oligonucleotides complementary to these oligonucleotides, and to the applications of this oligonucleotide for detecting enterobacteria.

Abstract: The invention relates to a fragment of the genomic DNA of Streptococcus pneumoniae, a probe capable of specifically hybridizing with the genomic DNA of Screptococcus pneumoniae, a specific primer for the amplification, by polymerization, of the genomic DNA, a reagent and a method which are used with the probe and, optionally, the primer, for specifically detecting Streptococcus pneumoniae in a biological sample. The probe of the invention is a nucleotide sequence having at least 70% homology with at least a portion of a consensus sequence of the genomic DNA of Streptococcus pneumoniae, this consensus sequence being chosen from the nucleotide sequences SEQ ID NO 2, SEQ ID NO 3, SEQ ID NO 4, which are identified in the description, and their respective complementary sequences.

Abstract: The invention relates to a fragment of the genomic DNA of Strepeococcus pneumoniae, a probe capable of specifically hybridizing with the genomic DNA of Streptococcus pneumoniae, a specific primer for the amplification, by polymerization, of the genomic DNA, a reagent and a method which are used with the probe and, optionally, the primer, for specifically detecting Streptococcus pneumoniae in a biological sample. The probe of the invention is a nucleotide sequence having at least 70% homology with at least a portion of a consensus sequence of the genomic DNA of Streptococcus pneumoniae, this consensus sequence being chosen from the nucleotide sequences SEQ ID NO 2, SEQ ID NO 3, SEQ ID NO 4, which are identified in the description, and their respective complementary sequences.

Abstract: Device for capturing a target molecule for the purpose of detecting it and/or assaying it, including a solid support on which is immobilized a ligand, the ligand being provided in the form of a conjugate resulting from the covalent coupling of a polymer with a plurality of molecules of the ligand. The polymer is an N-vinylpyrrolidone copolymer, and the conjugate is immobilized on the solid support by adsorption.When the ligand is capable of forming a complex with the target, the device is specific for a given target. When the device comprises, in addition, a bifunctional reagent capable of forming a complex, on the one hand, with the ligand and, on the other hand, with the target, the support on which the ligand is immobilized constitutes a universal capturing system.

Abstract: Bacteria of the genus Mycobacterium can be detected and/or identified by methods using probes including fragments of a variable region of a 23S ribosomal RNA of a species of the genus Mycobacterium or probes including DNA fragments that are obtained by reverse transcription of the RNA or that form the RNA by transcription. Primers for the reverse transcription of a variable region of a 23S ribosomal RNA sequence of mycobacteria include nucleotide sequences of a 23S RNA of a species of the genus Mycobacterium.

Abstract: A procedure for detecting single-stranded nucleotide sequences in a sample is disclosed wherein a passively fixed capture probe is used in concert with a non-radioactively labelled detection probe in a sandwich hybridization technique.

Abstract: The invention relates to a fragment of the genomic DNA of Streptococcus pneumoniae, a probe capable of specifically hybridizing with the genomic DNA of Streptococcus pneumoniae, a specific primer for the amplification, by polymerization, of the genomic DNA, a reagent and a method which are used with the probe and, optionally, the primer, for specifically detecting Streptococcus pneumoniae in a biological sample. The probe of the invention is a nucleotide sequence having at least 70% homology with at least a portion of a consensus sequence of the genomic DNA of Streptococcus pneumoniae, this consensus sequence being chosen from the nucleotide sequences SEQ ID NO 2, SEQ ID NO 3, SEQ ID NO 4, which are identified in the description, and their respective complementary sequences.

Abstract: Nucleotide fragment of DNA, whose nucleotide sequence is included in the gene of the species of the genus Mycobacterium, coding for the 65-kD mycobacterial antigen, containing regions which are homologous in practically all species of the genus Mycobacterium, and at least one species-specific variable region, characterized in that said fragment is chosen from fragments whose nucleotide sequences possess at least 70% homology, and preferably at least 85% homology, with a predetermined sequence or its complementary sequence, said predetermined sequence beginning at nucleotide 438 and ending at nucleotide 751 of said gene coding for said antigen of all species of mycobacteria except for the species M. tuberculosis, M. bovis BCG, M. avium, M. paratuberculosis, M. fortuitum, M. malmoense, M. leprae, M. kansaii and M. marinurn.