Abstract: The invention concerns an analysis card as well as a method for filing such a card and the use of said card. Said card comprises a body consisting of: a zone for injecting into the card a biological fluid to be analyzed; a main duct supplying the biological fluid coming from the injection zone; at least two secondary ducts located in the extension of the main duct; and at least one well corresponding to each secondary duct. The invention is characterized in that each well constitutes a means for dispensing a specific volume of said biological fluid into at least two terminal analysis ducts, via a terminal duct for each analysis cavity and the volume to be analyzed is less than the total volume of all the analysis ducts. Although this concerns a particular embodiment wherein a biological fluid is transferred in parallel, in another embodiment the transfer may be carried out in series. The invention is particularly applicable in microbiology.