Patents by Inventor Claudia Chiesa
Claudia Chiesa has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 6827830Abstract: An electrophoretic method for purifying a nucleic acid sample is disclosed. According to the method, the electrophoresis is effective to substantially reduce the concentration of contaminants relative to the concentration of desired nucleic acid in the nucleic acid sample, thereby producing a purified nucleic acid. In the method, the loading and recovery wells may be the same or different, and the electric fields may be DC or alternating.Type: GrantFiled: September 20, 2000Date of Patent: December 7, 2004Assignees: Applera Corporation, University of OttawaInventors: Gary Slater, J. William Efcavitch, Guy Drouin, Pascal Mayer, Jean Rousseau, Hong Yan Zhou, Claudia Chiesa, Robert Ruhfel, Roger O'Neill
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Patent number: 6787310Abstract: The present invention provides methods and kits for isolating one strand of a double-stranded target nucleic acid. The method capitalizes on the differences in the kinetics and thermodynamic stabilities between conventional DNA/DNA, DNA/RNA and RNA/RNA duplexes and heteroduplexes in which one strand of the heteroduplexe is a nucleobase polymer having a net positively charged or net neutral backbone, such as a PNA.Type: GrantFiled: July 18, 2001Date of Patent: September 7, 2004Assignee: Applera CorporationInventors: Claudia Chiesa, Gary P. Schroth, Michael Egholm
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Patent number: 6638760Abstract: The present invention provides substrates and apparatuses for efficient, rapid and specific capture, and optimal recovery, of nucleic acids, as well as methods of their use. The substrate is porous in nature and has a capture polynucleotide capable of hybridizing to a target nucleic acid immobilized thereon. Upon flowing a sample containing or suspected of containing the target nucleic acid through the porous substrate, the target nucleic acid is rapidly captured. Following capture, the target nucleic acid can be efficiently recovered for subsequent use.Type: GrantFiled: December 3, 1998Date of Patent: October 28, 2003Assignee: PE Corporation (NY)Inventors: Jer-Kang Chen, Claudia Chiesa, George A. Fry, Vergine C. Furniss, Stephen M. Lambert, Roger O'Neill, Majid Mehrpouyan
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Publication number: 20030148277Abstract: The present invention provides methods and kits for isolating one strand of a double-stranded target nucleic acid. The method capitalizes on the differences in the kinetics and thermodynamic stabilities between conventional DNA/DNA, DNA/RNA and RNA/RNA duplexes and heteroduplexes in which one strand of the heteroduplexe is a nucleobase polymer having a net positively charged or net neutral backbone, such as a PNA.Type: ApplicationFiled: July 18, 2001Publication date: August 7, 2003Inventors: Claudia Chiesa, Gary P. Schroth, Michael Egholm
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Patent number: 6514699Abstract: The invention relates to methods and compositions for simultaneously generating a plurality of polynucleotide sequencing ladders or PCR amplification products. Each sequencing ladder is generated from a recoverable primer, i.e., an oligonucleotide primer comprising a recovery tag. The recovery tag may be an oligonucleotide. Each sequencing ladder has a unique recovery tag. After the generation of the multiple sequencing ladders, the different sequencing ladders are separated from one another, i.e., purified, by binding to recovery tag binding compounds that have been immobilized on one or more solid supports. The recovery tag binding compounds are immobilized on the solid support in an addressable manner, i.e., the recovery tag binding compounds have distinct locations on the solid support. The binding of the sequencing ladders to the recovery tag binding compounds serves to separate the different polynucleotide sequencing ladders present in a given solution.Type: GrantFiled: June 13, 2000Date of Patent: February 4, 2003Assignee: PE Corporation (NY)Inventors: Roger A. O'Neill, Jer-Kang Chen, Claudia Chiesa, George Fry
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Patent number: 6297009Abstract: Disclosed is a method for increasing the electroosmotic flow rate available for a silica surface. In the method, there is provided an electrophoretic channel which is defined by one or more silica surfaces. The surface(s) are contacted with an alkaline aqueous solution containing a solubilized silicate-monovalent metal complex in an amount effective to increase the acidity of the silica surface(s), as evidenced by a reduction in the average bulk pKa of the surface(s). The achieved increase in acidity is greater than would be obtained using an otherwise identical solution lacking said silicate. In one preferred embodiment, the monovalent metal used in the solution is Li+, Na+, or K+. Also disclosed is a method for increasing the acidity of a silica surface, by contacting the surface with an alkaline aqueous solution of the type noted above.Type: GrantFiled: January 9, 1998Date of Patent: October 2, 2001Assignee: Perkin-Elmer CorporationInventors: David M. Demorest, Stephen E. Moring, Claudia Chiesa
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Patent number: 6146511Abstract: An electrophoretic method for purifying a nucleic acid sample is disclosed. The method generally comprises the steps of (1) providing a nucleic acid sample comprising a desired nucleic acid and one or more contaminants, (2) providing an electrophoresis matrix having a loading well and a recovery well formed therein, (3) placing the nucleic acid sample into the loading well, (4) performing a first electrophoresis comprising electrophoresing the nucleic acid sample for a first time effective to transport the desired nucleic acid out of the loading well and into the electrophoresis matrix; and (5) performing a second electrophoresis comprising electrophoresing the nucleic acid sample for a second time effective to transport the desired nucleic acid out of the electrophoresis matrix and into the recovery well.Type: GrantFiled: January 30, 1998Date of Patent: November 14, 2000Assignees: The Perkin-Elmer Corporation, The University of OttawaInventors: Gary Slater, J. William Efcavitch, Guy Drouin, Pascal Mayer, Jean Rousseau, Hong Yan Zhou, Claudia Chiesa, Robert Ruhfel, Roger O'Neill
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Patent number: 6124092Abstract: The invention relates to methods and compositions for simultaneously generating a plurality of polynucleotide sequencing ladders or PCR amplification products. Each sequencing ladder is generated from a recoverable primer, i.e., an oligonucleotide primer comprising a recovery tag. The recovery tag may be an oligonucleotide. Each sequencing ladder has a unique recovery tag. After the generation of the multiple sequencing ladders, the different sequencing ladders are separated from one another, i.e., purified, by binding to recovery tag binding compounds that have been immobilized on one or more solid supports. The recovery tag binding compounds are immobilized on the solid support in an addressable manner, i.e., the recovery tag binding compounds have distinct locations on the solid support. The binding of the sequencing ladders to the recovery tag binding compounds serves to separate the different polynucleotide sequencing ladders present in a given solution.Type: GrantFiled: June 12, 1997Date of Patent: September 26, 2000Assignee: The Perkin-Elmer CorporationInventors: Roger A. O'Neill, Jer-Kang Chen, Claudia Chiesa, George Fry
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Patent number: 5578179Abstract: Disclosed is a method for increasing the electro-osmotic flow rate available for a silica surface. In the method, there is provided an electrophoretic channel which is defined by one or more silica surfaces. The surface(s) are contacted with an alkaline aqueous solution containing a solubilized silicate-monovalent metal complex in an amount effective to increase the acidity of the silica surface(s), as evidenced by a reduction in the average bulk pKa of the surface(s). The achieved increase in acidity is greater than would be obtained using an otherwise identical solution lacking said silicate. In one preferred embodiment, the monovalent metal used in the solution is Li.sup.+, Na.sup.+, or K.sup.+. Also disclosed is a method for increasing the acidity of a silica surface, by contacting the surface with an alkaline aqueous solution of the type noted above.Type: GrantFiled: July 12, 1995Date of Patent: November 26, 1996Assignee: The Perkin-Elmer CorporationInventors: David M. Demorest, Stephen E. Moring, Claudia Chiesa