Patents by Inventor Claudia Muller
Claudia Muller has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20160151282Abstract: An amphoteric liposome composed of a mixture of lipids, said mixture comprising a cationic amphiphile, an anionic amphiphile and optionally one or more neutral amphiphiles, at least one of said cationic and anionic amphiphiles being chargeable and the respective amounts of said cationic and anionic amphiphiles being selected such there is a stoichiometric excess of positively charged cationic amphiphile at a first lower pH, a stoichiometric excess of negatively charged anionic amphiphile at a second higher pH and said mixture has an isoelectric point intermediate said first and second pHs; characterised in that said positively charged cationic and negatively charged anionic amphiphiles are adapted to form a lipid salt with one another at said isoelectric point. Also disclosed are methods of predicting the fusogenicity of an amphoteric liposome at a given pH, formulating an amphoteric liposome and loading an amphoteric liposome with a cargo moiety.Type: ApplicationFiled: January 31, 2016Publication date: June 2, 2016Inventors: Steffen Panzner, Silke Lutz, Evgenios Siepi, Claudia Muller
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Patent number: 9283186Abstract: An amphoteric liposome composed of a mixture of lipids, said mixture comprising a cationic amphiphile, an anionic amphiphile and optionally one or more neutral amphiphiles, at least one of said cationic and anionic amphiphiles being chargeable and the respective amounts of said cationic and anionic amphiphiles being selected such there is a stoichiometric excess of positively charged cationic amphiphile at a first lower pH, a stoichiometric excess of negatively charged anionic amphiphile at a second higher pH and said mixture has an isoelectric point intermediate said first and second pHs; characterised in that said positively charged cationic and negatively charged anionic amphiphiles are adapted to form a lipid salt with one another at said isoelectric point. Also disclosed are methods of predicting the fusogenicity of an amphoteric liposome at a given pH, formulating an amphoteric liposome and loading an amphoteric liposome with a cargo moiety.Type: GrantFiled: January 13, 2012Date of Patent: March 15, 2016Assignee: Marina Biotech, Inc.Inventors: Steffen Panzner, Silke Lutz, Evgenios Siepi, Claudia Müller
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Publication number: 20140178462Abstract: An amphoteric liposome comprising neutral lipids wherein said neutral lipids are selected from the group comprising cholesterol or mixtures of cholesterol and at least one neutral or zwitterionic lipid and wherein K (neutral) of said mixtures is 0.3 or less. Said amphoteric liposome may encapsulate an active agent, such as nucleic acid therapeutics. Also disclosed are pharmaceutical compositions comprising said amphoteric liposomes as a carrier for the delivery or targeted delivery of active agents or ingredients.Type: ApplicationFiled: October 9, 2013Publication date: June 26, 2014Applicant: Marina Biotech, Inc.Inventors: Steffen Panzner, Silke Lutz, Evgenios Siepi, Claudia Muller, Ute Vinzens
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Publication number: 20140056970Abstract: A method for preparing amphoteric liposomes loaded with a polyanionic active agent as cargo, characterised by admixing an aqueous solution of said polyanionic active agent and an alcoholic solution of one or more amphiphiles and buffering said admixture to an acidic pH, said one or more amphiphiles being susceptible of forming amphoteric liposomes at said acidic pH, thereby to form such amphoteric liposomes in suspension encapsulating said active agent under conditions such that said liposomes form aggregates, and thereafter treating said suspension to dissociate said aggregates. Also disclosed are nucleic acid loaded amphoteric liposomes produced in accordance with the method, wherein said nucleic acids are oligonucleotides and said liposomes are multilamellar.Type: ApplicationFiled: October 29, 2013Publication date: February 27, 2014Applicant: Marina Biotech, Inc.Inventors: Steffen Panzner, Gerold Endert, Una Rauchhaus, Natalie Herzog, Claudia Muller
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Patent number: 8193246Abstract: Lipid assemblies, such as liposomes, comprising transfection enhancer elements (TEE's), which are complexed with the lipid assemblies by means of ionic interactions, or lipids incorporating such TEE's are disclosed for enhancing the fusogenicity of the lipid assemblies. The TEE's have the formula: hydrophobic moiety-pH sensitive hydrophilic moiety??(II) The pH sensitive hydrophilic moiety of each TEE is a weak acid having a pka of between 2 and 6 or a zwitterionic structure comprising a combination of acidic groups with weak bases having a pKa of between 3 and 8. Lipids incorporating one or more such TEEs have the formula (I): Lipid moiety-[Hydrophobic moiety-pH sensitive hydrophilic moiety]??(I).Type: GrantFiled: December 19, 2007Date of Patent: June 5, 2012Assignee: Marina Biotech, Inc.Inventors: Steffen Panzner, Evgenios Siepi, Silke Lutz, Christian Reinsch, Claudia Müller
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Publication number: 20120135065Abstract: An amphoteric liposome composed of a mixture of lipids, said mixture comprising a cationic amphiphile, an anionic amphiphile and optionally one or more neutral amphiphiles, at least one of said cationic and anionic amphiphiles being chargeable and the respective amounts of said cationic and anionic amphiphiles being selected such there is a stoichiometric excess of positively charged cationic amphiphile at a first lower pH, a stoichiometric excess of negatively charged anionic amphiphile at a second higher pH and said mixture has an isoelectric point intermediate said first and second pHs; characterised in that said positively charged cationic and negatively charged anionic amphiphiles are adapted to form a lipid salt with one another at said isoelectric point. Also disclosed are methods of predicting the fusogenicity of an amphoteric liposome at a given pH, formulating an amphoteric liposome and loading an amphoteric liposome with a cargo moiety.Type: ApplicationFiled: January 13, 2012Publication date: May 31, 2012Inventors: Steffen Panzner, Silke Lutz, Evgenios Siepi, Claudia Müller
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Publication number: 20120021042Abstract: A method for preparing amphoteric liposomes loaded with polyanionic active agent as cargo, characterised by admixing an aqueous solution of said polyanionic active agent and an alcoholic solution of one or more amphiphiles and buffering said admixture to an acidic pH, said one or more amphiphiles being susceptible of forming amphoteric liposomes at said acidic pH, thereby to form such amphoteric liposomes in suspension encapsulating said active agent under conditions such that said liposomes form aggregates, and thereafter treating said suspension to dissociate said aggregates. Also disclosed are nucleic acid loaded amphoteric liposomes produced in accordance with the method, wherein said nucleic acids are oligonucleotides and said liposomes are multilamellar.Type: ApplicationFiled: March 16, 2007Publication date: January 26, 2012Inventors: Steffen Panzner, Gerold Endert, Una Rauchhaus, Natalie Herzog, Claudia Muller
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Publication number: 20100330154Abstract: An amphoteric liposome comprising neutral lipids wherein said neutral lipids are selected from the group comprising cholesterol or mixtures of cholesterol and at least one neutral or zwitterionic lipid and wherein K (neutral) of said mixtures is 0.3 or less. Said amphoteric liposome may encapsulate an active agent, such as nucleic acid therapeutics. Also disclosed are pharmaceutical compositions comprising said amphoteric liposomes as a carrier for the delivery or targeted delivery of active agents or ingredients.Type: ApplicationFiled: October 12, 2008Publication date: December 30, 2010Inventors: Steffen Panzner, Silke Lutz, Evgenios Siepi, Claudia Muller, Ute Vinzens
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Publication number: 20080306153Abstract: Lipid assemblies, such as liposomes, comprising transfection enhancer elements (TEE's), which are complexed with the lipid assemblies by means of ionic interactions, or lipids incorporating such TEE's are disclosed for enhancing the fusogenicity of the lipid assemblies. The TEE's have the formula: hydrophobic moiety-pH sensitive hydrophilic moiety??(II) The pH sensitive hydrophilic moiety of each TEE is a weak acid having a pka of between 2 and 6 or a zwitterionic structure comprising a combination of acidic groups with weak bases having a pKa of between 3 and 8.Type: ApplicationFiled: December 19, 2007Publication date: December 11, 2008Inventors: Steffen Panzner, Evgenios Siepi, Silke Lutz, Christian Reinsch, Claudia Muller
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Publication number: 20080089932Abstract: An amphoteric liposome composed of a mixture of lipids, said mixture comprising a cationic amphiphile, an anionic amphiphile and optionally one or more neutral amphiphiles, at least one of said cationic and anionic amphiphiles being chargeable and the respective amounts of said cationic and anionic amphiphiles being selected such there is a stoichiometric excess of positively charged cationic amphiphile at a first lower pH, a stoichiometric excess of negatively charged anionic amphiphile at a second higher pH and said mixture has an isoelectric point intermediate said first and second pHs; characterised in that said positively charged cationic and negatively charged anionic amphiphiles are adapted to form a lipid salt with one another at said isoelectric point. Also disclosed are methods of predicting the fusogenicity of an amphoteric liposome at a given pH, formulating an amphoteric liposome and loading an amphoteric liposome with a cargo moiety.Type: ApplicationFiled: October 12, 2007Publication date: April 17, 2008Inventors: Steffen Panzner, Silke Lutz, Evgenios Siepi, Claudia Muller
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Publication number: 20070154953Abstract: The present invention relates to methods for identifying and/or detecting T-cell epitopes of a protein antigen, to methods for preparing peptide vaccines against a protein antigen, to methods for controlling the quality of receptor/ligand complexes and/or their components, to methods for preparing nanoparticles having at least one immobilized receptor unit or an immobilized receptor, to methods for preparing nanoparticles having immobilized receptor/ligand complexes, in particular peptide-presenting MHC molecules, to methods for enriching and/or isolating specific CD4+-T- or CD8+-T-lymphocytes from peripheral blood mononuclear cells, to methods for priming a CD8+-T-lymphocyte reaction in vitro, to nanoparticles having an immobilized receptor unit, in particular an immobilized chain of an MHC molecule, to nanoparticles having an immobilized receptor, in particular an immobilized MHC molecule, to nanoparticles having an immobilized receptor/ligand complex, in particular a peptide-presenting MHC molecule, to a pType: ApplicationFiled: March 3, 2004Publication date: July 5, 2007Inventors: Herwig Brunner, Gunter Tover, Thomas Schiestel, Claudia Muller, Thomas Flad
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Patent number: 6627324Abstract: Described is a film having at least a single layer and at least one copolyamide layer (I) containing dispersed nanoscale nucleating particles. Crystalline structures extending from the dispersed particle surfaces are formed after the layer (I) is cooled from a molten state at a cooling rate of from 10° C. to 20° C. per minute. The dispersed nanoscale nucleating particles are present in layer (I) in an amount of from 10 ppm to 2000 ppm, based on the total weight of layer (I). Also described is a method of using the film of the present invention in packaging foodstuffs.Type: GrantFiled: April 10, 2001Date of Patent: September 30, 2003Assignee: Wolff Walsrode AGInventors: Holger Eggers, Gregor Kaschel, Claudia Müller, Andreas Gasse, Rainer Brandt, Dieter Neubauer, Bernd Eilers
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Patent number: 6576348Abstract: A multilayer film having a lamination side and a heat-sealable side is described. The multilayer film comprises, a) an inner layer (i) ethylene/vinyl alcohol copolymer; b) a coupling agent layer (ii) and a separate coupling agent layer (ii′), said inner layer (i) being interposed between and in contact with each of said coupling agent layer (ii) and said separate coupling agent layer (ii′); c) at least one further layer (iii), said separate coupling agent layer (ii′) being interposed between and in contact with each of said inner layer (i) and said layer (iii); d) at least one layer (iv), said coupling agent layer (ii) being interposed between and in contact with each of said inner layer (i) and said layer (iv); and e) a heat-sealable layer (v), said layer (iv) being interposed between and in contact with each of said coupling agent layer (ii) and said heat-sealable layer (v). Layers (i), (ii), (ii′), (iii) and (v) of the multilayer film contain 0.1 to 3 wt.Type: GrantFiled: April 4, 2001Date of Patent: June 10, 2003Assignee: Wolff Walsrode AGInventors: Holger Eggers, Rudi Klein, Claudia Müller, Rainer Brandt
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Publication number: 20020187342Abstract: A multilayer film having a lamination side and a heat-sealable side is described. The multilayer film comprises, a) an inner layer (i) ethylene/vinyl alcohol copolymer; b) a coupling agent layer (ii) and a separate coupling agent layer (ii′), said inner layer (i) being interposed between and in contact with each of said coupling agent layer (ii) and said separate coupling agent layer (ii′); c) at least one further layer (iii), said separate coupling agent layer (ii′) being interposed between and in contact with each of said inner layer (i) and said layer (iii); d) at least one layer (iv), said coupling agent layer (ii) being interposed between and in contact with each of said inner layer (i) and said layer (iv); and e) a heat-sealable layer (v), said layer (iv) being interposed between and in contact with each of said coupling agent layer (ii) and said heat-sealable layer (v). Layers (i), (ii), (ii′), (iii) and (v) of the multilayer film contain 0.1 to 3 wt.Type: ApplicationFiled: April 4, 2001Publication date: December 12, 2002Inventors: Holger Eggers, Rudi Klein, Claudia Muller, Rainer Brandt
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Patent number: 6485406Abstract: The present invention relates to new radioactive iodine-125 miniature radiation sources (seeds) comprising a radioactive carrier matrix which consists of a porous and mechanically stable inorganic material, the pores of which contain inorganic insoluble iodide-125, and which is enclosed within a capsule made of a corrosion-resistent and body-compatible material or which is coated with such a material. The iodine seeds according to the present invention further may contain (a) usual X-ray marker(s). Another object of the present invention are methods for manufacturing said novel iodine-125 seeds.Type: GrantFiled: October 21, 1999Date of Patent: November 26, 2002Assignee: Eurotope Entwicklungsgesellschaft fur Istopentechnologien mbHInventors: Jürgen Ziegler, Claudia Müller, Gunnar Mann, André Hess
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Patent number: 5750761Abstract: A process for the preparation of compounds of the formula ##STR1## wherein the substituents X, Y and Z have the meaning given in the description, by reaction of compounds of the formula ##STR2## with a cyanide and an oxidizing agent other than Br.sub.2 in the presence of halides which offers industrial hygiene advantages and renders possible large-scale industrial access to dyestuff syntheses has been found.Type: GrantFiled: February 28, 1996Date of Patent: May 12, 1998Assignee: Bayer AktiengesellschaftInventors: Rainer Hamprecht, Claudia Muller