Patents by Inventor Claudio Ghetti

Claudio Ghetti has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for the identification of sensory neuron subtypes in ex vivo preparations
    Patent number: 10955409
    Abstract: The present invention provides methods of identifying and distinguishing different types of nerve cells in ex vivo cell culture, the method comprising the steps of: a) culturing somato-sensory nerve cells ex vivo, b) loading the nerve cells with a calcium, sodium, or voltage-sensitive indicator or expressing a genetically encoded calcium, sodium, or voltage-sensitive indicator, c) pulsing the nerve cells with an electrical train of bipolar square waves at two different voltages and two different frequencies; wherein the first voltage is 10 V/cm or less (low voltage) and the second voltage is between 12 and 20 V/cm (high voltage); and wherein the first frequency is 5 Hz or less (low frequency) and the second frequency is between 15 and 20 Hz (high frequency), and d) detecting activation of the nerve cell by measuring the changes in the signal intensity of the indicator, wherein low voltage and low frequency activation indicates a first type of cell and activation detected only at high voltage indicates a secon
    Type: Grant
    Filed: April 19, 2018
    Date of Patent: March 23, 2021
    Assignee: AnaBios Corporation
    Inventors: Andrea Ghetti, Yannick Miron, Claudio Ghetti, Paul Miller
  • METHOD FOR THE IDENTIFICATION OF SENSORY NEURON SUBTYPES IN EX VIVO PREPARATIONS
    Publication number: 20180306781
    Abstract: The present invention provides methods of identifying and distinguishing different types of nerve cells in ex vivo cell culture, the method comprising the steps of: a) culturing somato-sensory nerve cells ex vivo, b) loading the nerve cells with a calcium, sodium, or voltage-sensitive indicator or expressing a genetically encoded calcium, sodium, or voltage-sensitive indicator, c) pulsing the nerve cells with an electrical train of bipolar square waves at two different voltages and two different frequencies; wherein the first voltage is 10 V/cm or less (low voltage) and the second voltage is between 12 and 20 V/cm (high voltage); and wherein the first frequency is 5 Hz or less (low frequency) and the second frequency is between 15 and 20 Hz (high frequency), and d) detecting activation of the nerve cell by measuring the changes in the signal intensity of the indicator, wherein low voltage and low frequency activation indicates a first type of cell and activation detected only at high voltage indicates a secon
    Type: Application
    Filed: April 19, 2018
    Publication date: October 25, 2018
    Applicant: AnaBios Corporation
    Inventors: Andrea Ghetti, Yannick Miron, Claudio Ghetti, Paul Miller