Patents by Inventor Clemens Hendricus M. Kocken

Clemens Hendricus M. Kocken has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Efficient expression of apical membrane antigen in yeast cells
    Patent number: 7910330
    Abstract: A method of efficiently expressing Plasmodium AMA-1 ectodomain or a functional part, derivative, and/or analogue thereof, in a eukaryotic expression system. Preferably, the Plasmodium AMA-1 ectodomain is Pf AMA-1 ectodomain. This protein may be expressed in yeast, such as Pichia pastoris. Efficient expression is possible using a method for producing mRNA encoding the Plasmodium AMA-1 ectodomain in a yeast cell, comprising providing the yeast cell with a nucleic acid encoding Plasmodium AMA-1 ectodomain, the nucleic acid being modified to utilize the yeast cell's codon usage. Preferably, at least one putative yeast polyadenylation consensus sequence in the nucleic acid has been modified. More preferably, also at least one site in the protein that is generally glycosylated by eukaryotic expression systems, has been removed.
    Type: Grant
    Filed: July 8, 2003
    Date of Patent: March 22, 2011
    Assignee: Stichting Biomedical Primate Research Centre
    Inventors: Clemens Hendricus M. Kocken, Alan William Thomas, Michael John Blackman, Chrislaine Withers-Martinez, Anthony Arthur Holder
  • Efficient expression of Plasmodium apical membrane antigen in yeast cells
    Publication number: 20040091971
    Abstract: A method of efficiently expressing Plasmodium AMA-1 ectodomain or a functional part, derivative and/or analogue thereof in a eukaryotic expression system. Preferably, the Plasmodium AMA-1 ectodomain is Pf AMA-1 ectodomain. This protein may be expressed in yeast, such as Pichia pastoris. Efficient expression is possible using a method for producing mRNA encoding said Plasmodium AMA-1 ectodomain in a yeast cell, comprising providing the yeast cell with a nucleic acid encoding Plasmodium AMA-1 ectodomain, the nucleic acid being modified to utilize the yeast's codon usage. Preferably, at least one putative yeast polyadenylation consensus sequence in the nucleic acid has been modified. More preferably, also at least one site in the protein that is generally glycosylated by eukaryotic expression systems, has been removed.
    Type: Application
    Filed: July 8, 2003
    Publication date: May 13, 2004
    Inventors: Clemens Hendricus, M. Kocken, Alan William Thomas, Michael John Blackman, Chrislaine Withers-Martinez, Anthony Arthur Holder