Patents by Inventor Clement S. Chu
Clement S. Chu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240117343Abstract: Methods and compositions are provided for preparing DNA libraries. Enzymes, adaptors, and sample nucleic acids are provided in a single reaction mixture to facilitate library preparation.Type: ApplicationFiled: October 19, 2023Publication date: April 11, 2024Applicant: Myriad Women's Health, Inc.Inventors: Henry H. Lai, Clement S. Chu
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Patent number: 11932910Abstract: The present disclosure relates to methods for detecting unique genetic signatures derived from markers such as, for example, mutations, somatic or germ-line, in nucleic acids obtained from biological samples. The sensitivity of the methods provides for detection of mutations associated with a disease, e.g., cancer mutations, or with inherited disease, e.g., an autosomal recessive disease, in a noninvasive manner at ultra-low proportions of sequences carrying mutations to sequences carrying normal, e.g., non-cancer sequences, or a reference sequence, e.g., a human reference genome.Type: GrantFiled: July 22, 2021Date of Patent: March 19, 2024Assignee: Myriad Women's Health, Inc.Inventors: Jared Robert Maguire, Clement S. Chu, Imran Saeedul Haque, Eric Andrew Evans, Noah Welker
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Publication number: 20240076750Abstract: The present disclosure relates to a laboratory execution system that provides for automation of laboratory processes. A centralized data management system may be dynamically updated and used to facilitate management of components of the laboratory execution system, such as an automation system and an analytics results management system that may facilitate complex analytical functions, such as synthesizing raw test data. Potential workflows include the detection of specific molecules of interest.Type: ApplicationFiled: November 8, 2023Publication date: March 7, 2024Applicant: Myriad Women's Health, Inc.Inventors: Jared Robert Maguire, Clement S. Chu, Imran Saeedul Haque, Eric Andrew Evans, Noah Welker
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Patent number: 11820980Abstract: Methods and compositions are provided for preparing DNA libraries. Enzymes, adaptors, and sample nucleic acids are provided in a single reaction mixture to facilitate library preparation.Type: GrantFiled: April 27, 2020Date of Patent: November 21, 2023Assignee: Myriad Women's Health, Inc.Inventors: Henry H. Lai, Clement S. Chu
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Publication number: 20230295716Abstract: The present disclosure relates to a laboratory execution system that provides for automation of laboratory processes. A centralized data management system may be dynamically updated and used to facilitate management of components of the laboratory execution system, such as an automation system and an analytics results management system that may facilitate complex analytical functions, such as synthesizing raw test data. Potential workflows include the detection of specific molecules of interest.Type: ApplicationFiled: August 8, 2022Publication date: September 21, 2023Applicant: MYRIAD WOMEN'S HEALTH, INC.Inventors: Jared Robert Maguire, Clement S. Chu, Imran Saeedul Haque, Eric Andrew Evans, Noah Welker
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Publication number: 20220396785Abstract: Provided are methods and compositions for enriching cfDNA fragments from a biological fluid sample. A biological fluid sample, such as a urine sample, is collected and, in certain examples, pretreated before enrichment of the cfDNA. For the pretreatment, the sample is centrifuged to remove large cells and large cellular debris. As part of the pretreatment, the sample is also cleared of additional large cellular debris and excess volume by subjecting the sample to anion exchange chromatography and eluting bound DNA. Following any pretreatment of the sample, different concentrations an alcoholic solution are used—along with a mixture of DNA-binding particles and a chaotropic agent—to enrich the sample with cfDNA fragments having different sizes. For example, a biological sample can be enriched with small cfDNA fragments less than about 100 base pairs in length or large cfDNA fragments greater than about 100 base pairs in length.Type: ApplicationFiled: April 8, 2022Publication date: December 15, 2022Applicant: MYRIAD WOMEN'S HEALTH, INC.Inventors: Noah C. Welker, Clement S. Chu
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Publication number: 20220333188Abstract: High-fidelity, high-throughput nucleic acid sequencing enables healthcare practitioners and patients to gain insight into genetic variants and potential health risks. However, previous methods of nucleic acid sequencing often introduce sequencing errors (for example, mutations that arise during the preparation of a nucleic acid library, during amplification, or sequencing). Provided herein are methods and compositions for sequencing nucleic acids. Further provided are methods of identifying an error in a nucleic acid sequence.Type: ApplicationFiled: April 22, 2022Publication date: October 20, 2022Applicant: Myriad Women's Health, Inc.Inventors: Clement S. Chu, Noah C. Welker, Henry H. Lai
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Publication number: 20220290229Abstract: The present disclosure relates to methods for detecting unique genetic signatures derived from markers such as, for example, mutations, somatic or germ-line, in nucleic acids obtained from biological samples. The sensitivity of the methods provides for detection of mutations associated with a disease, e.g., cancer mutations, or with inherited disease, e.g., an autosomal recessive disease, in a noninvasive manner at ultra-low proportions of sequences carrying mutations to sequences carrying normal, e.g., non-cancer sequences, or a reference sequence, e.g., a human reference genome.Type: ApplicationFiled: February 23, 2022Publication date: September 15, 2022Applicant: MYRIAD WOMEN'S HEALTH, INC.Inventors: Jared Robert Maguire, Clement S. Chu, Imran Saeedul Haque, Eric Andrew Evans, Noah Welker
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Patent number: 11339431Abstract: High-fidelity, high-throughput nucleic acid sequencing enables healthcare practitioners and patients to gain insight into genetic variants and potential health risks. However, previous methods of nucleic acid sequencing often introduce sequencing errors (for example, mutations that arise during the preparation of a nucleic acid library, during amplification, or sequencing). Provided herein are methods and compositions for sequencing nucleic acids. Further provided are methods of identifying an error in a nucleic acid sequence.Type: GrantFiled: July 14, 2020Date of Patent: May 24, 2022Assignee: Myriad Women's Health, Inc.Inventors: Clement S. Chu, Noah C. Welker, Henry H. Lai
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Patent number: 11326158Abstract: Provided are methods and compositions for enriching cfDNA fragments from a biological fluid sample. A biological fluid sample, such as a urine sample, is collected and, in certain examples, pretreated before enrichment of the cfDNA. For the pretreatment, the sample is centrifuged to remove large cells and large cellular debris. As part of the pretreatment, the sample is also cleared of additional large cellular debris and excess volume by subjecting the sample to anion exchange chromatography and eluting bound DNA. Following any pretreatment of the sample, different concentrations an alcoholic solution are used—along with a mixture of DNA-binding particles and a chaotropic agent—to enrich the sample with cfDNA fragments having different sizes. For example, a biological sample can be enriched with small cfDNA fragments less than about 100 base pairs in length or large cfDNA fragments greater than about 100 base pairs in length.Type: GrantFiled: July 12, 2019Date of Patent: May 10, 2022Assignee: Myriad Women's Health, Inc.Inventors: Noah C. Welker, Clement S. Chu
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Publication number: 20210180050Abstract: High-fidelity, high-throughput nucleic acid sequencing enables healthcare practitioners and patients to gain insight into genetic variants and potential health risks. However, previous methods of nucleic acid sequencing often introduces sequencing errors (for example, mutations that arise during the preparation of a nucleic acid library, during amplification, or sequencing). Provided herein are methods and compositions for sequencing nucleic acids. Further provided are methods of identifying an error in a nucleic acid sequence.Type: ApplicationFiled: February 26, 2021Publication date: June 17, 2021Inventors: Henry H. Lai, Clement S. Chu
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Patent number: 10968447Abstract: High-fidelity, high-throughput nucleic acid sequencing enables healthcare practitioners and patients to gain insight into genetic variants and potential health risks. However, previous methods of nucleic acid sequencing often introduces sequencing errors (for example, mutations that arise during the preparation of a nucleic acid library, during amplification, or sequencing). Provided herein are methods and compositions for sequencing nucleic acids. Further provided are methods of identifying an error in a nucleic acid sequence.Type: GrantFiled: January 17, 2018Date of Patent: April 6, 2021Assignee: MYRIAD WOMEN'S HEALTH, INC.Inventors: Henry H. Lai, Clement S. Chu
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Publication number: 20210024992Abstract: High-fidelity, high-throughput nucleic acid sequencing enables healthcare practitioners and patients to gain insight into genetic variants and potential health risks. However, previous methods of nucleic acid sequencing often introduce sequencing errors (for example, mutations that arise during the preparation of a nucleic acid library, during amplification, or sequencing). Provided herein are methods and compositions for sequencing nucleic acids. Further provided are methods of identifying an error in a nucleic acid sequence.Type: ApplicationFiled: July 14, 2020Publication date: January 28, 2021Inventors: Clement S. Chu, Noah C. Welker, Henry H. Lai
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Patent number: 10752946Abstract: High-fidelity, high-throughput nucleic acid sequencing enables healthcare practitioners and patients to gain insight into genetic variants and potential health risks. However, previous methods of nucleic acid sequencing often introduce sequencing errors (for example, mutations that arise during the preparation of a nucleic acid library, during amplification, or sequencing). Provided herein are methods and compositions for sequencing nucleic acids. Further provided are methods of identifying an error in a nucleic acid sequence.Type: GrantFiled: January 17, 2018Date of Patent: August 25, 2020Assignee: MYRIAD WOMEN'S HEALTH, INC.Inventors: Clement S. Chu, Noah C. Welker, Henry H. Lai
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Publication number: 20200255824Abstract: Methods and compositions are provided for preparing DNA libraries. Enzymes, adaptors, and sample nucleic acids are provided in a single reaction mixture to facilitate library preparation.Type: ApplicationFiled: April 27, 2020Publication date: August 13, 2020Inventors: Henry H. Lai, Clement S. Chu
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Publication number: 20200082908Abstract: Described are methods for selecting an amount of a critical parameter (such as an amount of a sequencing library, amount of a capture probe library, or a number of amplification cycles) for direct targeted sequencing.Type: ApplicationFiled: August 30, 2019Publication date: March 12, 2020Inventors: Henry Lai, Clement S. Chu
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Publication number: 20190367905Abstract: Provided are methods and compositions for enriching cfDNA fragments from a biological fluid sample. A biological fluid sample, such as a urine sample, is collected and, in certain examples, pretreated before enrichment of the cfDNA. For the pretreatment, the sample is centrifuged to remove large cells and large cellular debris. As part of the pretreatment, the sample is also cleared of additional large cellular debris and excess volume by subjecting the sample to anion exchange chromatography and eluting bound DNA. Following any pretreatment of the sample, different concentrations an alcoholic solution are used—along with a mixture of DNA-binding particles and a chaotropic agent—to enrich the sample with cfDNA fragments having different sizes. For example, a biological sample can be enriched with small cfDNA fragments less than about 100 base pairs in length or large cfDNA fragments greater than about 100 base pairs in length.Type: ApplicationFiled: July 12, 2019Publication date: December 5, 2019Applicant: MYRIAD WOMEN'S HEALTH, INC.Inventors: Noah C. WELKER, Clement S. CHU
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Publication number: 20180346963Abstract: Methods for preparing concatenated nucleic acid molecules are provided. The methods herein include adaptors with complementary sequences for preparation of concatenated nucleic acid molecules, and methods of sequencing such nucleic acids.Type: ApplicationFiled: May 31, 2018Publication date: December 6, 2018Inventors: Noah C. Welker, Clement S. Chu
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Publication number: 20180216103Abstract: High-fidelity, high-throughput nucleic acid sequencing enables healthcare practitioners and patients to gain insight into genetic variants and potential health risks. However, previous methods of nucleic acid sequencing often introduces sequencing errors (for example, mutations that arise during the preparation of a nucleic acid library, during amplification, or sequencing). Provided herein are methods and compositions for sequencing nucleic acids. Further provided are methods of identifying an error in a nucleic acid sequence.Type: ApplicationFiled: January 17, 2018Publication date: August 2, 2018Inventors: Henry H. Lai, Clement S. Chu
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Publication number: 20180216253Abstract: Disclosed are high concentration reagents for use in preparing DNA samples in low volume reactions. Such reagents include, for example, DNA end repair buffers for use in low volume DNA blunting and phosphorylating reactions, DNA adenylating buffers for use in a low volume DNA adenylating reaction, and DNA ligation buffers for use in low volume DNA adaptor ligation reactions with adaptors. Also disclosed are customized reagent plates and kits containing one or more of these low volume buffers for use in low volume DNA blunting, phosphorylating, adenylating, and ligation reactions. Methods of using the high concentration reagents (low volume buffers) and the customized reagent plates for preparing DNA sequencing libraries in low volume reactions are also disclosed.Type: ApplicationFiled: January 29, 2018Publication date: August 2, 2018Applicant: Counsyl, Inc.Inventors: Henry H. Lai, Clement S. Chu