Abstract: The present disclosure provides modules, instruments and methods to enrich for cells edited via nucleic acid-guided nuclease editing of live cells.

Abstract: A hearing assistance system is disclosed that comprises a hearing assistance device configured to reproduce sounds and to assist a person to hear the sounds, the hearing assistance device including a left hearing assistance device and a right hearing assistance device, and a computing device in communication with the hearing assistance device. The computing device interacts with the hearing assistance device to implement a fitting mode wherein the hearing assistance device is caused to generate fitting sounds usable to evaluate whether the left and right hearing devices are properly fitted into respective left and right ears of the person. The computing device produces a communication indicative of whether the left and right hearing assistance devices are properly fitted into respective left and right ears of the person.

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells. The compositions and methods presented herein in combination lead to a phenomenon of “edit or die.” Although less cells survive plating and editing, a large percentage of cells that do survive are multiple editors. In one experiment it was found that if a cell survives transformation, plating, and editing, 75% of the surviving cells are multiple editors; that is, 75% of the surviving cells were simultaneously edited with edits at two or more different locations within the bacterial genome.

Abstract: The present disclosure provides compositions of matter, methods, modules and automated multi-module instrumentation for performing editing of live cells followed by curing of editing and engine vectors from prior rounds of editing, followed by curing of the curing vector.

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells. The compositions and methods presented herein in combination lead to a phenomenon of “edit or die.” Although less cells survive plating and editing, a large percentage of cells that do survive are multiple editors.

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells. The compositions and methods presented herein in combination lead to a phenomenon of “edit or die.” Although less cells survive plating and editing, a large percentage of cells that do survive are multiple editors. In one experiment it was found that if a cell survives transformation, plating, and editing, 75% of the surviving cells are multiple editors; that is, 75% of the surviving cells were simultaneously edited with edits at two or more different locations within the bacterial genome.

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells. The compositions and methods presented herein in combination lead to a phenomenon of “edit or die.” Although less cells survive plating and editing, a large percentage of cells that do survive are multiple editors.

Abstract: The present disclosure provides compositions of matter, methods, modules and automated multi-module instrumentation for performing editing of live cells followed by curing of editing and engine vectors from prior rounds of editing, followed by curing of the curing vector.

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells.

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells.

Abstract: A vehicle wiring harness assembly for installing an aftermarket electronic device into a vehicle electrical system includes a relay plug that is insertable into a starter relay socket in a vehicle electrical system fuse box. The relay plug takes the place of the starter relay in the vehicle electrical system fuse box. A fuse plug is insertable into a fuse slot in the vehicle electrical system fuse box. Thus, the fuse plug is in electrical communication with the battery of the vehicle electrical system. A wiring harness is electrically coupled to each of the relay plug and the fuse plug. In this way the wiring harness can supply voltage and ground to an aftermarket electronic device without modifying existing wiring in the vehicle electrical system.

Abstract: The present disclosure provides modules, instruments and methods to enrich for cells edited via nucleic acid-guided nuclease editing of live cells.

Abstract: The present disclosure provides methods, automated modules, and instruments for enrichment of live cells that have been edited by nucleic acid-guided nuclease genome editing. The disclosure provides improved methods and modules—including high throughput methods and modules—for enriching for cells that have been subjected to editing.

Abstract: The present disclosure thus provides methods for higher efficiency cell editing that maintain the viability of the cells. In certain aspects, the present disclosure provides systems and instruments for automated methods of multiplexed nuclease-directed genome editing using nuclease expression under a constitutive promoter. In specific aspects, the constitutive promoter is an insulated promoter. In other aspects, the constitutive promoter is a heterologous promoter. In still other aspects, the constitutive promoter is a synthetic promoter.

Abstract: A system and method for determining a state of a traffic signal. In one embodiment, a traffic signal state detector includes a lens, a color sensor, and a processor. The color sensor is configured to identify a plurality of colors of light directed to the color sensor by the lens, and a field of view of the color sensor is restricted based on dimensions of a traffic signal. The processor is coupled to the color sensor. The processor is configured to determine a color of light emitted by the traffic signal based on the colors of light identified by the color sensor.

Abstract: A system and method for determining a state of a traffic signal. In one embodiment, a traffic signal state detector includes a lens, a color sensor, and a processor. The color sensor is configured to identify a plurality of colors of light directed to the color sensor by the lens, and a field of view of the color sensor is restricted based on dimensions of a traffic signal. The processor is coupled to the color sensor. The processor is configured to determine a color of light emitted by the traffic signal based on the colors of light identified by the color sensor.

Abstract: In accordance with one exemplary embodiment, the present invention provides a sensor system for detecting a change in condition. The system includes a radioactive source. This system also includes at least one radiation detector configured to detect radiation from the radioactive source. Furthermore, the system includes a displacement assembly configured to physically displace in response to the change in condition and configured to change the radiation levels detected by the radiation detector in response to such displacement. Advantageously, this system facilitates non-intrusive communication of information across a physical barrier, via the measurement of changing radiation levels that change in response to a change in condition, for example.

Abstract: In accordance with one exemplary embodiment, the present invention provides a sensor system for detecting a change in condition. The system includes a radioactive source. This system also includes at least one radiation detector configured to detect radiation from the radioactive source. Furthermore, the system includes a displacement assembly configured to physically displace in response to the change in condition and configured to change the radiation levels detected by the radiation detector in response to such displacement. Advantageously, this system facilitates non-intrusive communication of information across a physical barrier, via the measurement of changing radiation levels that change in response to a change in condition, for example.

Abstract: A method for seismic exploration using nonlinear conversions between electromagnetic and seismic energy, with particular attention to the electromagnetic source waveform used. According to the invention, seismic returns from a source waveform are correlated with a reference waveform, with both waveforms custom designed to minimize both correlation side lobes and interference from linear electroseismic effects. A waveform element is selected which may be sequenced by a binary or similar digital code embodying the desired custom design to generate an input sweep with the needed depth penetration and noise suppression. Correlation of the seismic response with the reference waveform in a data processing step mathematically aggregates the seismic response from the input sweep into a single wavelet. Preferred binary digital codes include prescribed variations of maximal length shift-register sequences. Also, an apparatus for generating the desired waveforms.

Abstract: A method for seismic exploration using nonlinear conversions between electromagnetic and seismic energy, with particular attention to the electromagnetic source waveform used. According to the invention, seismic returns from a source waveform are correlated with a reference waveform, with both waveforms custom designed to minimize both correlation side lobes and interference from linear electroseismic effects. A waveform element is selected which may be sequenced by a binary or similar digital code embodying the desired custom design to generate an input sweep with the needed depth penetration and noise suppression. Correlation of the seismic response with the reference waveform in a data processing step mathematically aggregates the seismic response from the input sweep into a single wavelet. Preferred binary digital codes include prescribed variations of maximal length shift-register sequences. Also, an apparatus for generating the desired waveforms.