Abstract: A method is provided for building improved calibration models or for improving modifications to these models or validation of the models used in the non-invasive spectroscopic measurement of an analyte or attribute of tissue. The method uses a matched reference sample and measurement of that sample to ensure that the correct relationship between the spectra and analyte is made during the model building, modification or calibration process. A matched reference sample is one in which the analyte of interest or attribute of interest in the reference sample is representative of the analyte or attribute at the site being non-invasively sampled or the agreement between the reference concentration and the non-invasively sampled concentration is clinically significant.

Abstract: The present invention provides a method for using infrared light either alone or in conjunction with visible light to create an improved system for cytologic examination. As stated above, infrared light is sensitive to molecular and biochemical changes and can thus be used to identify specific chemical species or substances. This characteristic can be used to identify areas on a slide most likely to contain an abnormality, and can further provide information to overlay on an image of the slide to enhance the visual contrast for the human screener. This process of image enhancement via the use of multivariate spectroscopy can be used to dramatically enhance the information content provided to the medical professional.

Abstract: A method is provided for building improved calibration models or for improving modifications to these models or validation of the models used in the non-invasive spectroscopic measurement of an analyte or attribute of tissue. The method uses a matched reference sample and measurement of that sample to ensure that the correct relationship between the spectra and analyte is made during the model building, modification or calibration process. A matched reference sample is one in which the analyte of interest or attribute of interest in the reference sample is representative of the analyte or attribute at the site being non-invasively sampled or the agreement between the reference concentration and the non-invasively sampled concentration is clinically significant.