Patents by Inventor Clive Gavin Brown
Clive Gavin Brown has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20180030505Abstract: The present invention is a method for measuring the amount of at least one molecule in a biological sample, the method comprising a) combining the sample, or a derivative thereof, with one or more aptamers and allowing one or more molecules in the sample to bind to the aptamer(s); b) separating bound from unbound molecules; and c) quantifying the molecule(s) bound to the or each aptamer, wherein quantification of the bound molecule(s) is carried out by sequencing at least part of the or each aptamer. Uses of and products derived from the method are also contemplated.Type: ApplicationFiled: August 4, 2017Publication date: February 1, 2018Applicant: Caris Life Sciences Switzerland Holdings GmbHInventors: Clive Gavin BROWN, Koen KAS, Sven Agnes Jan EYCKERMAN
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Publication number: 20170350859Abstract: An analysis instrument comprises plural modules connected together over a data network, each module comprising an analysis apparatus operable to perform biochemical analysis of a sample. Each module comprises a control unit that controls the operation of the analysis apparatus. The control units are addressable to select an arbitrary number of modules to operate as a cluster for performing a common biochemical analysis. The control units communicate over the data network, repeatedly during the performance of the common biochemical analysis, to determine the operation of the analysis apparatus of each module required to meet the global performance targets, on the basis of measures of performance derived from the output data produced by the modules. The arrangement of the instrument as modules interacting in this manner provides a scalable analysis instrument.Type: ApplicationFiled: April 19, 2017Publication date: December 7, 2017Applicant: Oxford Nanopore Technologies Ltd.Inventors: Clive Gavin Brown, James Peter Willcocks
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Patent number: 9823235Abstract: The invention provides a method of forming a membrane between a first volume of polar medium and a second volume of polar medium. In some embodiments, the method involves providing a first volume including polar medium and a second volume including polar medium which are separated from one another by an apolar medium, in which at least one of the first and second volumes has a layer including amphipathic molecules.Type: GrantFiled: October 23, 2013Date of Patent: November 21, 2017Assignee: Oxford Nanopre Technologies Ltd.Inventors: Andrew John Heron, Jason Robert Hyde, Clive Gavin Brown
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Publication number: 20170326550Abstract: A detachable electrical device can be formed from a kit comprising a pair of component parts adapted for connection to each other, wherein the connected components of the device may be subsequently disconnected, comprising: an array of electrical connectors, each electrical connector comprising an electrically conductive liquid; and an array of electrodes; wherein the arrays can be brought into contact with each other so as to provide a plurality of electrical connections between the electrically conductive liquid of the array of electrical connectors and the electrodes of the array of electrodes, and wherein the electrical connections may be subsequently broken by detaching the electrically conductive liquid from the electrodes of the array.Type: ApplicationFiled: October 15, 2015Publication date: November 16, 2017Applicant: Oxford Nanopore Technologies Ltd.Inventors: Clive Gavin Brown, Jason Robert Hyde, Mark David Jackson, Paul Raymond Mackett, Jonathan Edward Mckendry, Richard Kenneth John Wiltshire
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Patent number: 9758811Abstract: The present invention is a method for measuring the amount of at least one molecule in a biological sample, the method comprising a) combining the sample, or a derivative thereof, with one or more aptamers and allowing one or more molecules in the sample to bind to the aptamer(s); b) separating bound from unbound molecules; and c) quantifying the molecule(s) bound to the or each aptamer, wherein quantification of the bound molecule(s) is carried out by sequencing at least part of the or each aptamer. Uses of and products derived from the method are also contemplated.Type: GrantFiled: September 5, 2014Date of Patent: September 12, 2017Assignee: Caris Life Sciences Switzerland Holdings GmbHInventors: Clive Gavin Brown, Koen Kas, Sven Agnes Jan Eyckerman
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Publication number: 20170253910Abstract: The invention relates to a new method of delivering an analyte to a transmembrane pore in a membrane. The method involves the use of microparticles.Type: ApplicationFiled: October 6, 2015Publication date: September 7, 2017Applicant: Oxford Nanopore Technologies Ltd.Inventors: Clive Gavin Brown, Daniel Ryan Garalde, Andrew John Heron, Daniel John Turner, James White
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Publication number: 20170233804Abstract: Analysis Of A Polymer A biochemical analysis system analyses polymers by taking measurements of a polymer from a sensor element comprising a nanopore during translocation of the polymer through the nanopore. When a polymer has partially translocated, the series of measurements is analysed using reference data derived from a reference sequence to provide a measure of similarity. Responsive to the measure of similarity, the sensor element may be selectively operated to eject the polymer and thereby make the nanopore available to receive a further polymer. Where the biochemical analysis system comprises an array of sensor elements and is takes measurements from sensor elements selected in a multiplexed manner, responsive to the measure of similarity, the biochemical analysis system ceases taking measurements from the currently selected sensor element and to starts taking measurements from a newly selected sensor element.Type: ApplicationFiled: October 16, 2015Publication date: August 17, 2017Applicant: Oxford Nanopore Technologies Ltd.Inventors: Stuart William Reid, Gavin Harper, Clive Gavin Brown, Daniel John Turner, Andrew John Heron, Christopher James Wright
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Publication number: 20170219557Abstract: A sequence of polymer units in a polymer (3), eg. DNA, is estimated from at least one series of measurements related to the polymer, eg. ion current as a function of translocation through a nanopore (1), FIG. 6 wherein the value of each measurement is dependent on a k-mer being a group of k polymer units (4). A probabilistic model, especially a hidden Markov model (HMM), is provided, comprising, for a set of possible k-mers: transition weightings representing the chances of transitions from origin k-mers to destination k-mers; and emission weightings in respect of each k-mer that represent the chances of observing given values of measurements for that k-mer. The series of measurements is analysed using an analytical technique, eg. Viterbi decoding, that refers to the model and estimates at least one estimated sequence of polymer units in the polymer based on the likelihood predicted by the model of the series of measurements being produced by sequences of polymer units.Type: ApplicationFiled: April 13, 2017Publication date: August 3, 2017Applicant: Oxford Nanopore Technologies Ltd.Inventors: Stuart William Reid, Gavin Harper, Clive Gavin Brown, James Anthony Clarke, Andrew John Heron
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Patent number: 9651519Abstract: An analysis instrument comprises plural modules connected together over a data network, each module comprising an analysis apparatus operable to perform biochemical analysis of a sample. Each module comprises a control unit that controls the operation of the analysis apparatus. The control units are addressable to select an arbitrary number of modules to operate as a cluster for performing a common biochemical analysis. The control units communicate over the data network, repeatedly during the performance of the common biochemical analysis, to determine the operation of the analysis apparatus of each module required to meet the global performance targets, on the basis of measures of performance derived from the output data produced by the modules. The arrangement of the instrument as modules interacting in this manner provides a scalable analysis instrument.Type: GrantFiled: July 29, 2015Date of Patent: May 16, 2017Assignee: Oxford Nanopore Technologies LimitedInventors: Clive Gavin Brown, James Peter Willcocks
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Publication number: 20170107569Abstract: The invention relates to new methods of attaching one or more polynucleotide binding proteins to a target polynucleotide. The invention also relates to new methods of characterising target polynucleotides.Type: ApplicationFiled: January 22, 2015Publication date: April 20, 2017Applicant: Oxford Nanopore Technologies Ltd.Inventors: Andrew John Heron, Clive Gavin Brown, Rebecca Victoria Bowen, James White, Daniel John Turner, Joseph Hargreaves Lloyd, Christopher Peter Youd
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Publication number: 20170058338Abstract: The invention relates to improving the movement of a target polynucleotide with respect to a transmembrane pore when the movement is controlled by a polynucleotide binding protein. The invention also relates to improved transmembrane pores and polynucleotide binding proteins.Type: ApplicationFiled: May 1, 2015Publication date: March 2, 2017Applicant: Oxford Nanopore Technologies Ltd.Inventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace, Jonathan Bankes Pugh, Richard George Hambley, Neil Roger Wood, Clive Gavin Brown, James White
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Patent number: 9551023Abstract: The invention relates to a method for modifying a template polynucleotide for characterization, especially for nanopore sequencing. The method produces a modified polynucleotide which is complementary to the template polynucleotide at some positions and which contains universal or abasic nucleotides at the other, and in some instances predicable, positions. The resulting modified polynucleotide can then be characterized.Type: GrantFiled: September 6, 2013Date of Patent: January 24, 2017Assignee: Oxford Nanopore Technologies Ltd.Inventors: Daniel John Turner, Clive Gavin Brown, Stuart William Reid, James Anthony Clarke, James White, David Jackson Stoddart
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Publication number: 20170002406Abstract: The invention relates to new methods of controlling the movement of polynucleotides through transmembrane pores. The invention also relates to new methods of characterising target polynucleotides using helicases.Type: ApplicationFiled: September 10, 2014Publication date: January 5, 2017Applicant: Oxford Nanopore Technologies Ltd.Inventors: Rebecca Victoria Bowen, Clive Gavin Brown, Mark Bruce, Andrew John Heron, Elizabeth Jayne Wallace, James White, Joseph Hargreaves Lloyd, David Antoni Alves, Domenico Caprotti, Lakmal Jayasinghe, Luke McNeill, John Milton, Antonino Puglisi, Szabolcs Soeroes
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Publication number: 20160362739Abstract: The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.Type: ApplicationFiled: August 24, 2016Publication date: December 15, 2016Applicant: Oxford Nanopore Technologies Ltd.Inventors: Clive Gavin Brown, James Anthony Clarke, Graham Hall, Gavin Harper, Andrew John Heron, James White
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Publication number: 20160281159Abstract: The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.Type: ApplicationFiled: June 10, 2016Publication date: September 29, 2016Applicant: Oxford Nanopore Technologies Ltd.Inventors: Clive Gavin Brown, James Anthony Clarke, Graham Hall, Gavin Harper, Andrew John Heron, James White
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Publication number: 20160251710Abstract: The invention relates to a new method of characterising a target ribonucleic acid (RNA) involving forming a complementary polynucleotide. The method uses a transmembrane pore.Type: ApplicationFiled: October 17, 2014Publication date: September 1, 2016Applicant: Oxford Nanopore Technologies Ltd.Inventors: Clive Gavin Brown, Daniel John, James White
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Publication number: 20160162634Abstract: A sequence of polymer units in a polymer (3), eg. DNA, is estimated from at least one series of measurements related to the polymer, eg. ion current as a function of translocation through a nanopore (1), wherein the value of each measurement is dependent on a k-mer being a group of k polymer units (4). A probabilistic model, especially a hidden Markov model (HMM), is provided, comprising, for a set of possible k-mers: transition weightings representing the chances of transitions from origin k-mers to destination k-mers; and emission weightings in respect of each k-mer that represent the chances of observing given values of measurements for that k-mer. The series of measurements is analysed using an analytical technique, eg. Viterbi decoding, that refers to the model and estimates at least one estimated sequence of polymer units in the polymer based on the likelihood predicted by the model of the series of measurements being produced by sequences of polymer units.Type: ApplicationFiled: September 21, 2012Publication date: June 9, 2016Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITEDInventors: Stuart William Reid, Gavin Harper, Clive Gavin Brown, James Anthony Clarke, Andrew John Heron
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Patent number: 9315804Abstract: The present invention is a method for the identification of one or more aptamers to at least one target molecule, the method comprising: selecting candidate aptamer sequences that bind to a target molecule, assigning to the bound sequences a measure (fitness function) of each sequence's aptameric potential, allowing evolution of some or all of the sequences to create a new mixture of candidate sequences, and repeating the method with the newly created candidate aptamer pool until the aggregate aptameric potential of the candidate pool reaches a plateau, wherein sequences present in the final pool are optimal aptamers to the target molecule.Type: GrantFiled: October 22, 2008Date of Patent: April 19, 2016Assignee: Caris Life Sciences Switzerland Holdings, GmbHInventor: Clive Gavin Brown
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Publication number: 20150346149Abstract: An analysis instrument comprises plural modules connected together over a data network, each module comprising an analysis apparatus operable to perform biochemical analysis of a sample. Each module comprises a control unit that controls the operation of the analysis apparatus. The control units are addressable to select an arbitrary number of modules to operate as a cluster for performing a common biochemical analysis. The control units communicate over the data network, repeatedly during the performance of the common biochemical analysis, to determine the operation of the analysis apparatus of each module required to meet the global performance targets, on the basis of measures of performance derived from the output data produced by the modules. The arrangement of the instrument as modules interacting in this manner provides a scalable analysis instrument.Type: ApplicationFiled: July 29, 2015Publication date: December 3, 2015Inventors: Clive Gavin BROWN, James Peter WILLCOCKS
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Publication number: 20150285781Abstract: The invention provides a method of forming a membrane between a first volume of polar medium and a second volume of polar medium, which method comprises: (a) providing a first volume comprising polar medium and a second volume comprising polar medium which are separated from one another by an apolar medium, wherein at least one of said first and second volumes comprises a layer comprising amphipathic molecules, at the interface between the polar medium and the apolar medium, wherein each of the amphipathic molecules comprises a first outer hydrophilic group, a hydrophobic core group, and a second outer hydrophilic group, wherein each of the first and second outer hydrophilic groups is linked to the hydrophobic core group; and (b) causing the first and second volumes to come into contact with one another to form a membrane comprising said amphipathic molecules between the first and second volumes.Type: ApplicationFiled: October 23, 2013Publication date: October 8, 2015Applicant: Oxford Nanopore Technologies Ltd.Inventors: Andrew John Heron, Jason Robert Hyde, Clive Gavin Brown