Abstract: A method of label-free cell or particle sorting in a microfluidic device includes providing a stream of aqueous droplets in oil in a channel of the microfluidic device, wherein at least some of the droplets include cells or particles and illuminating the stream from a first direction. Then, one detects scattered light from cells or particles within said aqueous droplets in a second direction. Next, one determines a number of the cells or particles in each droplet from the scattered light and sorts the aqueous droplets into differentiated streams. Notably, the refractive index of said oil is modified to closely match a refractive index of the aqueous droplets to reduce light scattered from boundaries of the droplets. A volume of the droplets can also controlled.

Abstract: A multi-ply fibrous product includes at least two fibrous plies, such as a multi-ply nonwoven product, a tissue paper product or a hybrid thereof including at least two tissue paper plies and/or nonwoven plies including cellulosic fibers. At least two fibrous plies are bonded to each other by an aqueous adhesive composition including an adhesive component and a dermatologically acceptable acid, and optionally a salt thereof. A process for the manufacture of this multi-ply fibrous product includes (a) providing at least two fibrous webs, (c) applying an aqueous adhesive composition including an adhesive component and a water-soluble, dermatologically acceptable acid, and optionally the corresponding salt thereof, to at least one side of at least one fibrous web, d) superimposing at least two webs such that the aqueous adhesive composition is located between at least two superimposed webs, and e) bonding at least two webs together.

Abstract: Instruments and cartridges for processing droplets in emulsions containing biological entities such as cells. A method of such processing comprises providing a plurality of the entities in a fluid; preparing a droplet from the fluid; determining whether the droplet contains one or more entities of said plurality of entities, or whether said droplet does not contain a said entity; sorting said droplet dependent on an outcome of the determination; and dispensing the sorted droplet into a reservoir. The dispensing may comprise identifying and extracting the sorted droplet from a first fluidic flow path of said fluid by transferring the sorted droplet from into a second fluidic flow path and then ejecting the sorted droplet into a reservoir by applying pressure to the second fluidic flow path. The droplet contents may be tracked so that the contents of an individual droplet can be sorted, selectively dispensed, and retrieved.

Abstract: A system for transporting fluid cargo within a shipping container, the system comprising an open topped box having side walls and end walls and a flexible membrane which either independently or in combination with the box contains the fluid cargo. The box supports the portion of the cargo within the box and is designed to flex and absorb at least part of the horizontal forces generated within the cargo during its transportation thus preventing overloading of the walls of the surrounding container. The membrane may be a bladder which completely encloses the cargo and is disposed partially within the box to contain the cargo independently of the box. The box may have a floor pan which can seal the box against leakage of any cargo.

Abstract: A frame system for supporting an associated vehicle for transport within an existing transportable structure having longitudinally extending side structures. The frame system has a transverse supporting frame supported by two vertical posts located one at each end of the supporting frame. Each post has an attachment for securing the top and bottom of the post at a plurality of locations along the length of the associated side structure and the supporting frame is raiseable with or without a vehicle loaded thereon and once raised is fixable to the posts at a chosen location for transport within the transportable structure. Each end of the support frame has an end plate for sliding up and down an inwardly facing surface on the adjacent vertical post as it is raised and lowered. At least one of the end plates has a telescopic connection with the remainder of the support frame.

Abstract: The present invention provides an emulsion for sorting droplets in a microfluidic device, the emulsion comprising: a discontinuous aqueous phase; and a continuous oil phase; wherein said aqueous phase comprises at least one analyte, said oil phase comprises a fluorous oil and a refractive index modifying compound comprising at least one aromatic ring, and the refractive index of said aqueous phase and the refractive index of said oil phase are substantially matched.

Abstract: Systems that employ microdroplets are used in embodiments for Microdroplet Electrospray Ionisation Mass Spectrometry (ESI MS). Thus, a method of detecting an analyte includes providing an oil composition comprising oil and an aqueous microdroplet comprising the analyte, the oil composition comprising a surfactant to stabilise the aqueous microdroplet in the oil composition; and performing ionisation mass spectrometry analysis of the oil composition.

Abstract: We describe a method of label-free cell and/or particle sorting in a microfluidic device, the method comprising; providing a stream of aqueous droplets in oil in a channel of said microfluidic device, wherein at least some of said droplets include cells and/or particles illuminating said stream from a first direction; detecting, for example, scattered light from cells and/or particles within said aqueous droplets in a second direction; determining, for example, a number of said cells and/or particles in each droplet from the scattered light; and sorting said aqueous droplets into one of a plurality of differentiated streams responsive to said determined number. The method further comprises modifying a refractive index of said oil to more closely match a refractive index of said aqueous droplets to reduce light scattered from boundaries of said droplets. Optionally a volume of the droplets is also controlled. In embodiments the oil is fluorous oil.

Abstract: The present invention provides an emulsion for sorting droplets in a microfluidic device, the emulsion comprising: a discontinuous aqueous phase; and a continuous oil phase; wherein said aqueous phase comprises at least one analyte, said oil phase comprises a fluorous oil and a refractive index modifying compound comprising at least one aromatic ring, and the refractive index of said aqueous phase and the refractive index of said oil phase are substantially matched.

Abstract: A frame system for supporting an associated vehicle for transport within an existing transportable structure having longitudinally extending side structures. The frame system has a transverse supporting frame supported by two vertical posts located one at each end of the supporting frame. Each post has attachment means for securing the top and bottom of the post at a plurality of locations along the length of the associated side structure and the supporting frame is raiseable with or without a vehicle loaded thereon and once raised is fixable to the posts at a chosen location for transport within the transportable structure. Each end of the support frame has an end plate for sliding up and down an inwardly facing surface on the adjacent vertical post as it is raised and lowered. At least one of the end plates has a telescopic connection with the remainder of the support frame.

Abstract: A system for transporting fluid cargo within a shipping container, the system comprising an open topped box having side walls and end walls and a flexible membrane which either independently or in combination with the box contains the fluid cargo. The box supports the portion of the cargo within the box and is designed to flex and absorb at least part of the horizontal forces generated within the cargo during its transportation thus preventing overloading of the walls of the surrounding container. The membrane may be a bladder which completely encloses the cargo and is disposed partially within the box to contain the cargo independently of the box. The box may have a floor pan which can seal the box against leakage of any cargo.

Abstract: A flexible walled bag, tank (1), or flexitank, for shipping fluids, in particular liquids, disposed within an outer container (12) confines, and supportable by a container floor, side walls and end structures, an upper tank surface being constrained, loaded or biassed, by external restraints, such as depending local spaced intrusions or baffles (22) extending from one side towards the other.

Abstract: A frame system for supporting two wheels of an associated vehicle for transport within an existing transportable structure such as a standard ISO container which has longitudinally extending side structures 5, 6. The frame system comprising a transverse wheel supporting frame 41, 43 supported by two vertical posts 40, 42 located one at each end of the wheel supporting frame. Each post is provided with attachment means 44, 48a for securing the top and bottom of the post at a plurality of locations along the length of the associated side structure independent of the shape of the side structure. The wheel supporting frame 41, 43 is raiseable with or without a vehicle loaded thereon and once raised is fixable 49, 50, 51 to the posts 40, 42 at a chosen location for transport within the transportable structure. The attachment means may comprise ties 44 of adjustable length which pull each post in the fore and aft direction relative to the side structure 5, 6 to locate each post.

Abstract: Systems that employ microdroplets are used in embodiments for Microdroplet Electrospray Ionisation Mass Spectrometry (ESI MS). Thus, a method of detecting an analyte includes providing an oil composition comprising oil and an aqueous microdroplet comprising the analyte, the oil composition comprising a surfactant to stabilise the aqueous microdroplet in the oil composition; and performing ionisation mass spectrometry analysis of the oil composition.

Abstract: A frame system for supporting two wheels of an associated vehicle for transport within an existing transportable structure such as a standard ISO container which has longitudinally extending side structures 5, 6. The frame system comprising a transverse wheel supporting frame 41, 43 supported by two vertical posts 40, 42 located one at each end of the wheel supporting frame. Each post is provided with attachment means 44, 48a for securing the top and bottom of the post at a plurality of locations along the length of the associated side structure independent of the shape of the side structure. The wheel supporting frame 41, 43 is raiseable with or without a vehicle loaded thereon and once raised is fixable 49, 50, 51 to the posts 40, 42 at a chosen location for transport within the transportable structure. The attachment means may comprise ties 44 of adjustable length which pull each post in the fore and aft direction relative to the side structure 5, 6 to locate each post.

Abstract: A medical diagnostic and communications apparatus with audio output comprises an electronic processor for processing stethoscope signals and secondary audio signals. An electronic stethoscope sensor is contained within a housing for transducing body sounds to electronic signals, and is operatively connected to the electronic processor. One or more secondary audio signal sources operatively connects to the electronic processor. A common audio output is connected to electronic processor to convert electronic stethoscope signals or secondary audio signals to acoustic output. These sounds may be produced separately or mixed.

Abstract: A transducer system is disclosed for detecting actual or simulated body sounds. An audio signal generation and detection system is disclosed for the purposes of simulating the medical examination of a patient or simulating the listening of sounds seeming to emanate from a live or inanimate body. A signal generator sets up a voltage potential at an electrode physically attached to the body, or electrically connected to a body, thereby setting up a voltage potential on a surface area of the body. An electric field potential sensor or a capacitive electrical sensor placed in proximity to the electrode or body surface then detects the voltage potential. The signals produced by the signal generator can represent heart, lung, bowel or other sounds and the electrical sensor can take the physical form of a listening device such as a stethoscope, thereby creating a simulation of listening to body sounds for medical diagnostic purposes.

Abstract: A flexible walled bag, tank (1), or flexitank, for shipping fluids, in particular liquids, disposed within an outer container (12) confines. and supportable by a container floor, side walls and end structures, an upper tank surface being constrained. loaded or biassed, by external restraints, such as depending local spaced intrusions or baffles (22) extending from one side towards the other.

Abstract: An acoustic-to-electrical transducer for sensing body sounds is disclosed. The transducer comprises a diaphragm that can be placed in direct contact with a body, whereby the diaphragm motion directly affects an electromagnetic sensing signal, which is then converted to an electrical signal representation of the diaphragm motion. Such sensing means allows the diaphragm to move freely without mechanical coupling to a secondary transducer, while providing a direct and efficient acoustic to electrical conversion means. The transducer further provides a means for using static diaphragm pressure to control gain and frequency characteristics of the electrical signal. The transducer provides methods for ambient noise reduction or cancellation, as well as means for simulating sound detection for applications such as medical education and testing. The sensor, circuitry, manufacturing methods and improvements are disclosed.

Abstract: A vehicle support deck frame (10) is condfigured to allow wheel (12) penetration through the deck depth and projection somewhat below the deck underside, so that a projecting wheel and/or attendant tyre (13) acts as a resiliently deformable spacer, cushion, bolster or buffer, in relation to an underlying (vehicle) load (30); such a ‘sunken’ wheel disposition allows a vehicle to sit lower in relation to the deck, so reducing the load depth (31) on that deck.