Patents by Inventor Colin Hebert

Colin Hebert has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11561164
    Abstract: A microfluidic chip configuration wherein injection occurs in an upwards vertical direction, and fluid vessels are located below the chip in order to minimize particle settling before and at the analysis portion of the chip's channels. The input and fluid flow up through the bottom of the chip, in one aspect using a manifold, which avoids orthogonal re-orientation of fluid dynamics. The contents of the vial are located below the chip and pumped upwards and vertically directly into the first channel of the chip. A long channel extends from the bottom of the chip to near the top of the chip. Then the channel takes a short horizontal turn that nearly negates any influence of cell settling due to gravity and zero flow velocity at the walls. The fluid is pumped up to a horizontal analysis portion that is the highest channel/fluidic point in the chip and thus close to the top of the chip, which results in clearer imaging.
    Type: Grant
    Filed: April 22, 2021
    Date of Patent: January 24, 2023
    Assignee: LUMACTYE, INC.
    Inventors: Sean Hart, Colin Hebert, Christopher Field, Shweta Krishnan
  • Publication number: 20220146405
    Abstract: Provided are methods and devices for assessing biological particles for use in cell immunotherapy. By utilizing a microfluidic chip device together with optical force measurement and cell imaging, the methods enable comprehensive assessment and characterization of biological particles with regard to morphology, motility, binding affinities, and susceptibility to external forces, including but not limited to, chemical, biochemical, biological, physical and temperature influences. The methods enable the selection and production of biological particles, such as engineered T-cells, for use in immunotherapy and biomanufacturing.
    Type: Application
    Filed: January 26, 2022
    Publication date: May 12, 2022
    Inventors: Sean Hart, Colin Hebert
  • Publication number: 20220072541
    Abstract: Provided are devices for automated analysis of one or more samples in single or multi-well plates or vessels, wherein the process of automated analysis comprises automated flow, wherein the samples comprise liquid or particles in a sample vessel, and wherein the devices comprise an assembly of components that enable processing of a sample for analytical assessment by fluidic and/or particle based instruments. Automated flow may comprise systems for moving samples including vacuum systems, pressure-based systems, pneumatic systems, pumps, peristaltic pumps, diaphragms, or syringes. The devices may comprise an assembly of components that enable movement in X, Y, and Z dimensions, as well as switches, microfluidic tubing, well plate block, electronic pressure controllers, pneumatic or fluidic mixing devices, components for fluid handling, sampling vessels, and mechanical components for translating or transporting system components.
    Type: Application
    Filed: September 9, 2021
    Publication date: March 10, 2022
    Inventors: Sean Hart, Colin Hebert, Margaret McCoy, Shweta Krishnan, Christopher Field, Zachary Evans, Adam Lubrano, Nathan LaPuma
  • Publication number: 20220008922
    Abstract: Provided herein are devices, systems, and methods of using the same, that enable manual and automated sampling and preparation of biological samples for assessment. The samples may be obtained in any quantity, including nano/micro/millifluidic amounts. The samples comprise cells and/or other biological particles that are in suspension or grown on substrates such as microcarriers, and may be obtained from one or more containers, such as single well plates, vials, flasks or bioreactors. The instrument to which the sample is transferred may comprise any analytical instrument, such as an optical force or laser force cytology instrument.
    Type: Application
    Filed: July 8, 2021
    Publication date: January 13, 2022
    Inventors: Sean Hart, Colin Hebert
  • Publication number: 20210333194
    Abstract: A microfluidic chip configuration wherein injection occurs in an upwards vertical direction, and fluid vessels are located below the chip in order to minimize particle settling before and at the analysis portion of the chip's channels. The input and fluid flow up through the bottom of the chip, in one aspect using a manifold, which avoids orthogonal re-orientation of fluid dynamics. The contents of the vial are located below the chip and pumped upwards and vertically directly into the first channel of the chip. A long channel extends from the bottom of the chip to near the top of the chip. Then the channel takes a short horizontal turn that nearly negates any influence of cell settling due to gravity and zero flow velocity at the walls. The fluid is pumped up to a horizontal analysis portion that is the highest channel/fluidic point in the chip and thus close to the top of the chip, which results in clearer imaging.
    Type: Application
    Filed: April 22, 2021
    Publication date: October 28, 2021
    Inventors: Sean Hart, Colin Hebert, Christopher Field, Shweta Krishnan
  • Publication number: 20210223162
    Abstract: Provided are methods and devices for assessing biological particles for use in cell immunotherapy. By utilizing a microfluidic chip device together with optical force measurement and cell imaging, the methods enable comprehensive assessment and characterization of biological particles with regard to morphology, motility, binding affinities, and susceptibility to external forces, including but not limited to, chemical, biochemical, biological, physical and temperature influences. The methods enable the selection and production of biological particles, such as engineered T-cells, for use in immunotherapy and biomanufacturing.
    Type: Application
    Filed: December 21, 2020
    Publication date: July 22, 2021
    Inventors: Sean Hart, Colin Hebert
  • Patent number: 11041797
    Abstract: A microfluidic chip configuration wherein injection occurs in an upwards vertical direction, and fluid vessels are located below the chip in order to minimize particle settling before and at the analysis portion of the chip's channels. The input and fluid flow up through the bottom of the chip, in one aspect using a manifold, which avoids orthogonal re-orientation of fluid dynamics. The contents of the vial are located below the chip and pumped upwards and vertically directly into the first channel of the chip. A long channel extends from the bottom of the chip to near the top of the chip. Then the channel takes a short horizontal turn that nearly negates any influence of cell settling due to gravity and zero flow velocity at the walls. The fluid is pumped up to a horizontal analysis portion that is the highest channel/fluidic point in the chip and thus close to the top of the chip, which results in clearer imaging.
    Type: Grant
    Filed: December 23, 2017
    Date of Patent: June 22, 2021
    Assignee: LumaCyte, LLC
    Inventors: Sean Hart, Colin Hebert, Christopher Field, Shweta Krishnan
  • Publication number: 20210121878
    Abstract: Provided are methods and devices for automated analysis of one or more samples in single or multi-well plates or vessels, wherein the process of automated analysis comprises flow and hydrodynamic, electrokinetic, and optical forces for the analysis and sorting of samples, wherein the samples comprise liquid or particles in microfluidic channels, and wherein the devices comprise an assembly of components that enable processing of a said samples for analytical assessment by fluidic and/or particle based instruments. Microfluidic structures (channels, “T's”, “Y's”, branched “Y's”, wells, and weirs) are described for facilitating sample interaction and observation, sample analysis, sorting, or isolation. Detection can be accomplished using spectroscopic methods including, but not limited to, Raman spectroscopy of single cells and bulk cellular samples (collections of cells; several individuals to hundreds or thousands of cells).
    Type: Application
    Filed: September 9, 2020
    Publication date: April 29, 2021
    Inventors: Sean Hart, Colin Hebert
  • Publication number: 20210011018
    Abstract: The present invention is directed to intelligent algorithms, methodologies and computer-implemented methodologies for biophysical and biochemical cellular monitoring and quantification enabling enhanced performance and objective analysis of advanced infectivity assays including neutralization assays and adventitious agent testing using fluidic and optical force-based measurements.
    Type: Application
    Filed: March 20, 2019
    Publication date: January 14, 2021
    Inventors: Sean Hart, Colin Hebert, Margaret McCoy
  • Patent number: 10871440
    Abstract: Provided are methods and devices for assessing biological particles for use in cell immunotherapy. By utilizing a microfluidic chip device together with optical force measurement and cell imaging, the methods enable comprehensive assessment and characterization of biological particles with regard to morphology, motility, binding affinities, and susceptibility to external forces, including but not limited to, chemical, biochemical, biological, physical and temperature influences. The methods enable the selection and production of biological particles, such as engineered T-cells, for use in immunotherapy and biomanufacturing.
    Type: Grant
    Filed: April 25, 2019
    Date of Patent: December 22, 2020
    Assignee: Lumacyte, LLC
    Inventors: Sean Hart, Colin Hebert
  • Publication number: 20190308191
    Abstract: Provided are devices for automated analysis of one or more samples in single or multi-well plates or vessels, wherein the process of automated analysis comprises automated flow, wherein the samples comprise liquid or particles in a sample vessel, and wherein the devices comprise an assembly of components that enable processing of a sample for analytical assessment by fluidic and/or particle based instruments. Automated flow may comprise systems for moving samples including vacuum systems, pressure-based systems, pneumatic systems, pumps, peristaltic pumps, diaphragms, or syringes. The devices may comprise an assembly of components that enable movement in X, Y, and Z dimensions, as well as switches, microfluidic tubing, well plate block, electronic pressure controllers, pneumatic or fluidic mixing devices, components for fluid handling, sampling vessels, and mechanical components for translating or transporting system components.
    Type: Application
    Filed: April 8, 2019
    Publication date: October 10, 2019
    Inventors: Sean Hart, Colin Hebert, Margaret McCoy, Shewta Krishnan, Christopher Field, Zachary Evans, Adam Lubrano, Nathan LaPuma
  • Publication number: 20190250093
    Abstract: Provided are methods and devices for assessing biological particles for use in cell immunotherapy. By utilizing a microfluidic chip device together with optical force measurement and cell imaging, the methods enable comprehensive assessment and characterization of biological particles with regard to morphology, motility, binding affinities, and susceptibility to external forces, including but not limited to, chemical, biochemical, biological, physical and temperature influences. The methods enable the selection and production of biological particles, such as engineered T-cells, for use in immunotherapy and biomanufacturing.
    Type: Application
    Filed: April 25, 2019
    Publication date: August 15, 2019
    Inventors: Sean Hart, Colin Hebert
  • Publication number: 20190195773
    Abstract: A microfluidic chip configuration wherein injection occurs in an upwards vertical direction, and fluid vessels are located below the chip in order to minimize particle settling before and at the analysis portion of the chip's channels. The input and fluid flow up through the bottom of the chip, in one aspect using a manifold, which avoids orthogonal re-orientation of fluid dynamics. The contents of the vial are located below the chip and pumped upwards and vertically directly into the first channel of the chip. A long channel extends from the bottom of the chip to near the top of the chip. Then the channel takes a short horizontal turn that nearly negates any influence of cell settling due to gravity and zero flow velocity at the walls. The fluid is pumped up to a horizontal analysis portion that is the highest channel/fluidic point in the chip and thus close to the top of the chip, which results in clearer imaging.
    Type: Application
    Filed: December 23, 2017
    Publication date: June 27, 2019
    Inventors: Sean Hart, Colin Hebert, Christopher Field, Shweta Krishnan