Abstract: Methods, systems, and computer-readable media for persisting user settings for non-persistent application streaming environments are disclosed. An application streaming service may receive a selection of an application for a user and assign a compute instance to the user. An agent provided by the compute instance obtains a storage location of a user settings file associated with the user (e.g., based on use of the application during a previous session). The agent downloads the user settings file from the storage location to the compute instance and mounts the user settings file at a file system location associated with an execution environment of the compute instance. The application is executed and the user settings file is updated during use of the application. In response to termination of the session, the agent uploads the updated user settings file to a storage location. The user settings file is not persisted on the compute instance.