Abstract: The present invention provides a method for efficiently producing L-homophenylalanine and a strain producing L-homophenylalanine. In the present invention, a new route for the synthesis of L-homophenylalanine by a cascade enzymatic method using cheap benzaldehyde and pyruvic acid as raw materials is designed. By constructing the pathway-related enzymes into the same E. coli strain, a recombinant E. coli is obtained, with which L-homophenylalanine is catalytically produced through reaction in a 5 L reactor, with a yield of 100.9 g/L, a conversion rate of 94%, and ee>99%. Compared with the existing main methods for producing L-HPA, the production cost of L-homophenylalanine is greatly reduced. Thus, the present invention has good application prospects.

Abstract: A method for generating a digital human video based on a large model, an electronic device, and a storage medium are provided, which relate to a field of artificial intelligence technologies, and may be applied to scenarios such as video livestreaming, advertisement production, and e-commerce sales. The method includes: acquiring a requirement information including an action description information for describing a specified action video segment, and the action video segment represents a specified action of a target object; processing the requirement information using a first large model to obtain a target script, where the target script includes a target speech segment text matching the action description information; and processing the target script and the action video segment using a second large model to obtain a target video for displaying a target digital human performing a speech delivery based on the target speech segment text while performing the specified action.

Abstract: The present invention relates to a recombinant strain for producing shikimic acid, in which a target gene that regulates the asymmetric cell division and target genes that regulate the shikimic acid production are expressed The target gene that regulates the asymmetric cell division includes cytoskeletal protein PopZ coding gene popZ, and the target genes that regulate the shikimic acid production include DAHP synthase coding gene aroG, 3-dehydroquinate synthase coding gene aroB, and transketolase coding gene tktA. The recombinant strain of the present invention realizes the de novo synthesis of shikimic acid using glucose as a substrate, with a low cost. After fermentation with the strain in a 7.5 L fermentor, the highest production of shikimic acid is 88.1 g/L, the yield is 0.33 g/g, and the production intensity of shikimic acid is 1.1 g/L/h.

Abstract: The present invention provides recombinant Escherichia coli for producing glutarate, a construction method and use thereof. A double-plasmid recombinant bacterium is constructed through molecular biological means for co-expressing an aldehyde synthase (AAS) gene, an amine oxidase Mao (gene) and an aldehyde dehydrogenase (Glox) gene. The constructed expression plasmids are introduced into the Escherichia coli to reconstruct to obtain recombinant cells. A recombination strain for efficiently producing glutarate is obtained through amicillin resistance and kanamycin resistance combined plate screening. Efficient production of the glutarate is achieved by optimizing concentration of a substrate, cell concentration and a transformation temperature. L-lysine with a concentration of 30 g/L may be transformed into 19.

Abstract: Disclosed is an acid phosphatase mutant obtained from Pseudomonas aeruginosa having the amino acid sequence of SEQ ID NO:3 and method of using the mutant in the technical field of biological engineering. The disclosure provides a mutant of acid phosphatase PaAPaseMu3. By expressing the mutant of acid phosphatase PaAPaseMu3 in Escherichia coli and using a whole-cell conversion method, L-ascorbic acid is transformed into L-ascorbate-2-phosphate. Moreover, the problems of a high substrate cost, environmental pollution and the like are greatly reduced, laying a foundation for the industrial green production of L-ascorbate-2-phosphate.

Abstract: An image-guided robotic spine injection system includes an injection robot registered to an interoperative imaging system for real-time guidance. The system includes a guidance system to communicate with said injection robot and said interoperative imaging system during an injection procedure. The guidance system includes a preoperative injection plan based on preoperative imaging data of a subject's spine, and includes anatomical features identified as preoperative registration markers. The guidance system receives interoperative imaging data from said interoperative imaging system of said subject's spine. The guidance system receives an indication of anatomical features identified as interoperative registration markers that correspond in a one-to-one relationship to each of said preoperative registration markers. The guidance system registers said interoperative registration markers with said preoperative registration markers to transform said preoperative injection plan to an interoperative injection plan.

Abstract: This application provides a data processing method, a computer device, and a medium. The method is applied to a terminal device including a first client. The first client has a public client key and a private client key. The first client stores encrypted data that is obtained by encrypting the private client key according to an object password. The method includes receiving the object password; decrypting the encrypted data by using the received object password to obtain the private client key; signing the encrypted data based on the private client key to obtain a signature of the encrypted data; and transmitting the public client key, the encrypted data, and the signature of the encrypted data to a cloud device of the first client, the cloud device storing the encrypted data after verifying the encrypted data based on the public client key and the signature of the encrypted data.

Abstract: The present disclosure provides a method and a device for processing voice interaction, an electronic device and a storage medium. The method includes: determining a first integrity of a voice instruction from a user by using a pre-trained integrity detection model in response to detecting that the voice instruction from the user is not a high-frequency instruction; determining a waiting duration for the voice instruction based on the first integrity and a preset integrity threshold, wherein the waiting duration for the voice instruction indicates a length of period between a time when a voice interaction device determines that receiving the voice instruction is completed and a time when the voice interaction device performs an operation in response to the voice instruction of the user; and controlling the voice interaction device to respond to the voice instruction of the user based on the waiting duration.

Abstract: Integrated inductors are formed by arranging multiple vias to bracket a volume of semiconductor substrate, where each via includes a top metal pad and a bottom metal pad. The vias are alternately connected by way of the top and bottom pads to form an end-to-end current loop along a length of the volume of semiconductor substrate.

Abstract: The present disclosure provides an efficient vibration reduction and isolation base supported by a chained panel fluid bladder, including a chained panel, the vibration reduction fluid bladder, vertical limiting devices and a bottom plate. The chained panel is a discontinuous structure formed by connecting chained substructure panels in series by panel hinge devices. The vibration reduction fluid bladder and the vertical limiting devices are fixedly installed between the chained panel and the bottom plate. The chained panel is constructed based on the impedance mismatch principle and provided with a mechanical device. Mechanical vibration energy is dissipated twice by the chained panel and the vibration reduction fluid bladder, thereby greatly reducing influences of mechanical device operation on a hull structure.

Abstract: The invention provides a recombinant Escherichia coli strain for producing succinic acid and a construction method thereof. The by-product encoding genes in the E. coli strain FMME-N-2 are knocked out to obtain the E. coli strain FMME-N-5 (?focA-pflB-?ldhA-?pta-ackA); and the phosphoenolpyruvate carboxykinase pck derived from Actinobacillus succinogenes and the phosphite dehydrogenase ptxD derived from Pseudomonas stutzeri were overexpressed. The constructed plasmid pTrcHisA-pck-ptxD was introduced into the expression host E. coli FMME-N-5 (?focA-pflB-?ldhA-?pta-ackA), and the cells were screened in a plate containing ampicillin, to obtain an engineered strain E. coli FMME-N-5 (?focA-pflB-?ldhA-?pta-ackA)-pck-ptxD that can efficiently produce succinic acid. After fermentation by a two-stage fermentation strategy, the production of succinic acid reaches 137 g/L, the yield of succinic acid is up to 1 g/g glucose, and the space time yield is 1.

Abstract: The invention provides a method for preparing pyrrolidone, and the invention provides a method for catalytically preparing pyrrolidone with ?-aminobutyric acid in the presence of carnitine-CoA ligase CaiC. The carnitine-CoA ligase CaiC has an amino acid sequence as shown in SEQ ID NO:1. The ligase has catalytic activity in the cyclization of ?-aminobutyric acid to produce pyrrolidone. The carnitine-CoA ligase provided in the present invention affords a yield of pyrrolidone of 3.26 g/L and a molar yield of 39.53% in 24 h when ?-aminobutyric acid is used as a substrate, thus reducing the production period, improving the production of pyrrolidone, and accelerating the industrialization process of producing pyrrolidone by enzymatic conversion method.

Abstract: Disclosed are a method and system for evaluating sonar self-noise at a ship design stage. The method includes: building a ship structure full-scale geometric simulation model; acquiring loss factors and sonar transducer space outfitting acoustic absorption coefficient material parameters; acquiring mechanical excitation, hydrodynamic excitation, and propeller excitation; inputting the loss factors and the sonar transducer space outfitting acoustic absorption coefficient material parameters into an established statistical energy evaluation model, and applying a mechanical excitation to a face plate of foundation of the built ship structure full-scale geometric simulation model, applying a hydrodynamic excitation to the surface of a ship hull, and applying a propeller excitation to a stern shaft to perform calculation of sonar self-noise of a ship to obtain total spectral density level of the sonar self-noise; and evaluating spectral density level calculation results by index requirements.

Abstract: Disclosed are a method and system for evaluating sonar self-noise at a ship design stage. The method includes: building a ship structure full-scale geometric simulation model; acquiring loss factors and sonar transducer space outfitting acoustic absorption coefficient material parameters; acquiring mechanical excitation, hydrodynamic excitation, and propeller excitation; inputting the loss factors and the sonar transducer space outfitting acoustic absorption coefficient material parameters into an established statistical energy evaluation model, and applying a mechanical excitation to a face plate of foundation of the built ship structure full-scale geometric simulation model, applying a hydrodynamic excitation to the surface of a ship hull, and applying a propeller excitation to a stern shaft to perform calculation of sonar self-noise of a ship to obtain total spectral density level of the sonar self-noise; and evaluating spectral density level calculation results by index requirements.

Abstract: The present invention provides recombinant Escherichia coli for producing glutarate, a construction method and use thereof. A double-plasmid recombinant bacterium is constructed through molecular biological means for co-expressing an aldehyde synthase (AAS) gene, an amine oxidase Mao (gene) and an aldehyde dehydrogenase (Glox) gene. The constructed expression plasmids are introduced into the Escherichia coli to reconstruct to obtain recombinant cells. A recombination strain for efficiently producing glutarate is obtained through amicillin resistance and kanamycin resistance combined plate screening. Efficient production of the glutarate is achieved by optimizing concentration of a substrate, cell concentration and a transformation temperature. L-lysine with a concentration of 30 g/L may be transformed into 19.

Abstract: The present disclosure discloses a control method and a control apparatus for speech interaction. The detailed implementation solution of the control method for the speech interaction includes: collecting an audio signal; detecting a wake-up word in the audio signal to obtain a wake-up word result; and playing a prompt tone and/or executing a speech instruction in the audio signal based on the wake-up word result.

Abstract: The present invention relates to a recombinant strain for producing shikimic acid, in which a target gene that regulates the asymmetric cell division and target genes that regulate the shikimic acid production are expressed The target gene that regulates the asymmetric cell division includes cytoskeletal protein PopZ coding gene popZ, and the target genes that regulate the shikimic acid production include DAHP synthase coding gene aroG, 3-dehydroquinate synthase coding gene aroB, and transketolase coding gene tktA. The recombinant strain of the present invention realizes the de novo synthesis of shikimic acid using glucose as a substrate, with a low cost. After fermentation with the strain in a 7.5 L fermentor, the highest production of shikimic acid is 88.1 g/L, the yield is 0.33 g/g, and the production intensity of shikimic acid is 1.1 g/L/h.

Abstract: The present disclosure provides an efficient vibration reduction and isolation base supported by a chained panel fluid bladder, including a chained panel, the vibration reduction fluid bladder, vertical limiting devices and a bottom plate. The chained panel is a discontinuous structure formed by connecting chained substructure panels in series by panel hinge devices. The vibration reduction fluid bladder and the vertical limiting devices are fixedly installed between the chained panel and the bottom plate. The chained panel is constructed based on the impedance mismatch principle and provided with a mechanical device. Mechanical vibration energy is dissipated twice by the chained panel and the vibration reduction fluid bladder, thereby greatly reducing influences of mechanical device operation on a hull structure.

Abstract: The present disclosure discloses a control method and a control apparatus for speech interaction. The detailed implementation solution of the control method for the speech interaction includes: collecting an audio signal; detecting a wake-up word in the audio signal to obtain a wake-up word result; and playing a prompt tone and/or executing a speech instruction in the audio signal based on the wake-up word result.

Abstract: A method for synthetizing a speech includes: obtaining a source speech; suppressing a noise in the source speech based on an amplitude component and/or phase component of the source speech, to obtain a noise-reduced speech; performing a speech recognition process on the noise-reduced speech to obtain corresponding text information; inputting the text information of the noise-reduced speech and a preset tag into a trained acoustic model to obtain a predicted acoustic feature matching the text information; and generating a target speech based on the predicted acoustic feature.