Abstract: The invention provides a method for preparing pyrrolidone, and the invention provides a method for catalytically preparing pyrrolidone with ?-aminobutyric acid in the presence of carnitine-CoA ligase CaiC. The carnitine-CoA ligase CaiC has an amino acid sequence as shown in SEQ ID NO:1. The ligase has catalytic activity in the cyclization of ?-aminobutyric acid to produce pyrrolidone. The carnitine-CoA ligase provided in the present invention affords a yield of pyrrolidone of 3.26 g/L and a molar yield of 39.53% in 24 h when ?-aminobutyric acid is used as a substrate, thus reducing the production period, improving the production of pyrrolidone, and accelerating the industrialization process of producing pyrrolidone by enzymatic conversion method.

Abstract: Disclosed are a method and system for evaluating sonar self-noise at a ship design stage. The method includes: building a ship structure full-scale geometric simulation model; acquiring loss factors and sonar transducer space outfitting acoustic absorption coefficient material parameters; acquiring mechanical excitation, hydrodynamic excitation, and propeller excitation; inputting the loss factors and the sonar transducer space outfitting acoustic absorption coefficient material parameters into an established statistical energy evaluation model, and applying a mechanical excitation to a face plate of foundation of the built ship structure full-scale geometric simulation model, applying a hydrodynamic excitation to the surface of a ship hull, and applying a propeller excitation to a stern shaft to perform calculation of sonar self-noise of a ship to obtain total spectral density level of the sonar self-noise; and evaluating spectral density level calculation results by index requirements.

Abstract: This application relates to automated processes for assigning item containers, such as boxes, to a location on a shipping container, such as a pallet. For example, a computing device may receive an item assignment request identifying a plurality of item containers, and determines a subset of the plurality of item containers for assigning to a layer of a shipping container. Further, the computing device determines a plurality of positions and a plurality of orientations for each of the subset of the plurality of item containers. The computing device also assigns the subset of the plurality of item containers to the layer of the shipping container based on the plurality of positions and the plurality of orientations. The computing device further generates an item assignment response identifying the assignments of the plurality of item containers, and transits the item assignment response for display.

Abstract: This application relates to automated processes for assigning item containers, such as boxes, to a location on a shipping container, such as a pallet. For example, a computing device may receive an item assignment request identifying a plurality of item containers, and determines a subset of the plurality of item containers for assigning to a layer of a shipping container. Further, the computing device determines a plurality of positions and a plurality of orientations for each of the subset of the plurality of item containers. The computing device also assigns the subset of the plurality of item containers to the layer of the shipping container based on the plurality of positions and the plurality of orientations. The computing device further generates an item assignment response identifying the assignments of the plurality of item containers, and transits the item assignment response for display.

Abstract: Disclosed are a method and system for evaluating sonar self-noise at a ship design stage. The method includes: building a ship structure full-scale geometric simulation model; acquiring loss factors and sonar transducer space outfitting acoustic absorption coefficient material parameters; acquiring mechanical excitation, hydrodynamic excitation, and propeller excitation; inputting the loss factors and the sonar transducer space outfitting acoustic absorption coefficient material parameters into an established statistical energy evaluation model, and applying a mechanical excitation to a face plate of foundation of the built ship structure full-scale geometric simulation model, applying a hydrodynamic excitation to the surface of a ship hull, and applying a propeller excitation to a stern shaft to perform calculation of sonar self-noise of a ship to obtain total spectral density level of the sonar self-noise; and evaluating spectral density level calculation results by index requirements.

Abstract: The present invention provides recombinant Escherichia coli for producing glutarate, a construction method and use thereof. A double-plasmid recombinant bacterium is constructed through molecular biological means for co-expressing an aldehyde synthase (AAS) gene, an amine oxidase Mao (gene) and an aldehyde dehydrogenase (Glox) gene. The constructed expression plasmids are introduced into the Escherichia coli to reconstruct to obtain recombinant cells. A recombination strain for efficiently producing glutarate is obtained through amicillin resistance and kanamycin resistance combined plate screening. Efficient production of the glutarate is achieved by optimizing concentration of a substrate, cell concentration and a transformation temperature. L-lysine with a concentration of 30 g/L may be transformed into 19.

Abstract: The present disclosure discloses a control method and a control apparatus for speech interaction. The detailed implementation solution of the control method for the speech interaction includes: collecting an audio signal; detecting a wake-up word in the audio signal to obtain a wake-up word result; and playing a prompt tone and/or executing a speech instruction in the audio signal based on the wake-up word result.

Abstract: The present invention relates to a recombinant strain for producing shikimic acid, in which a target gene that regulates the asymmetric cell division and target genes that regulate the shikimic acid production are expressed The target gene that regulates the asymmetric cell division includes cytoskeletal protein PopZ coding gene popZ, and the target genes that regulate the shikimic acid production include DAHP synthase coding gene aroG, 3-dehydroquinate synthase coding gene aroB, and transketolase coding gene tktA. The recombinant strain of the present invention realizes the de novo synthesis of shikimic acid using glucose as a substrate, with a low cost. After fermentation with the strain in a 7.5 L fermentor, the highest production of shikimic acid is 88.1 g/L, the yield is 0.33 g/g, and the production intensity of shikimic acid is 1.1 g/L/h.

Abstract: The present disclosure provides an efficient vibration reduction and isolation base supported by a chained panel fluid bladder, including a chained panel, the vibration reduction fluid bladder, vertical limiting devices and a bottom plate. The chained panel is a discontinuous structure formed by connecting chained substructure panels in series by panel hinge devices. The vibration reduction fluid bladder and the vertical limiting devices are fixedly installed between the chained panel and the bottom plate. The chained panel is constructed based on the impedance mismatch principle and provided with a mechanical device. Mechanical vibration energy is dissipated twice by the chained panel and the vibration reduction fluid bladder, thereby greatly reducing influences of mechanical device operation on a hull structure.

Abstract: The present disclosure discloses a control method and a control apparatus for speech interaction. The detailed implementation solution of the control method for the speech interaction includes: collecting an audio signal; detecting a wake-up word in the audio signal to obtain a wake-up word result; and playing a prompt tone and/or executing a speech instruction in the audio signal based on the wake-up word result.

Abstract: A method for synthetizing a speech includes: obtaining a source speech; suppressing a noise in the source speech based on an amplitude component and/or phase component of the source speech, to obtain a noise-reduced speech; performing a speech recognition process on the noise-reduced speech to obtain corresponding text information; inputting the text information of the noise-reduced speech and a preset tag into a trained acoustic model to obtain a predicted acoustic feature matching the text information; and generating a target speech based on the predicted acoustic feature.

Abstract: The present disclosure provides a method for a human-computer interaction, an apparatus for a human-computer interaction, a device, and a storage medium, and the present disclosure relates to the field of artificial intelligence, such as deep learning and voice. A specific implementation includes: acquiring a voice command; performing voice recognition on the voice command to determine a corresponding voice text; sending, in response to satisfying a preset information sending condition, the voice text to a cloud; receiving a resource for the voice command returned from the cloud; and responding to the voice command based on the resource.

Abstract: A method for generating a speech package, an electronic device and a storage medium The method includes: determining a number of texts to be displayed and a speech recording condition based on a type of a recording mode selection control in response to the recording mode selection control being triggered; acquiring speech data with an amount matched with the number based on the speech recording condition; sending the speech data to a server; and acquiring a speech package generated by the server using the speech data.

Abstract: The invention provides a recombinant Escherichia coli strain for producing succinic acid and a construction method thereof. The by-product encoding genes in the E. coli strain FMME-N-2 are knocked out to obtain the E. coli strain FMME-N-5 (?focA-pflB-?ldhA-?pta-ackA); and the phosphoenolpyruvate carboxykinase pck derived from Actinobacillus succinogenes and the phosphite dehydrogenase ptxD derived from Pseudomonas stutzeri were overexpressed. The constructed plasmid pTrcHisA-pck-ptxD was introduced into the expression host E. coli FMME-N-5 (?focA-pflB-?ldhA-?pta-ackA), and the cells were screened in a plate containing ampicillin, to obtain an engineered strain E. coli FMME-N-5 (?focA-pflB-?ldhA-?pta-ackA)-pck-ptxD that can efficiently produce succinic acid. After fermentation by a two-stage fermentation strategy, the production of succinic acid reaches 137 g/L, the yield of succinic acid is up to 1 g/g glucose, and the space time yield is 1.

Abstract: The embodiments of the present disclosure provides a smart service method, apparatus and device, the method includes: a first device plays response information corresponding to an awaking word after receiving the awaking word; when the first device determines that a second device in an awake state exists in a device group that the first device is in, the first device transmits operation information of the first device to the second device, and receives operation information of the second device transmitted by the second device; the first device determines, according to the operation information of the first device and the operation information of the second device a target device from the first device and the second device, where the target device is a device in the device group to provide a smart service. The service resources are economized.

Abstract: Disclosed is a mutant of acid phosphatase and an application thereof, belonging to the technical field of biological engineering. The disclosure provides a mutant of acid phosphatase PaAPaseMu3. By expressing the mutant of acid phosphatase PaAPaseMu3 in Escherichia coli and using a whole-cell conversion method, L-ascorbic acid is transformed into L-ascorbate-2-phosphate. Moreover, a catalytic system of the mutant of acid phosphatase PaAPaseMu3 is optimized from the aspects of reaction pH and temperature, so that a yield of L-ascorbate-2-phosphate can reach 90.1 g/L and a molar yield can reach 75.1%. Therefore, the problems of a high substrate cost, environmental pollution and the like before are greatly reduced, laying a foundation for the industrial green production of L-ascorbate-2-phosphate.

Abstract: The present disclosure provides a method and a device for processing voice interaction, an electronic device and a storage medium. The method includes: determining a first integrity of a voice instruction from a user by using a pre-trained integrity detection model in response to detecting that the voice instruction from the user is not a high-frequency instruction; determining a waiting duration for the voice instruction based on the first integrity and a preset integrity threshold, wherein the waiting duration for the voice instruction indicates a length of period between a time when a voice interaction device determines that receiving the voice instruction is completed and a time when the voice interaction device performs an operation in response to the voice instruction of the user; and controlling the voice interaction device to respond to the voice instruction of the user based on the waiting duration.

Abstract: The present disclosure discloses a control method and a control apparatus for speech interaction. The detailed implementation solution of the control method for the speech interaction includes: collecting an audio signal; detecting a wake-up word in the audio signal to obtain a wake-up word result; and playing a prompt tone and/or executing a speech instruction in the audio signal based on the wake-up word result.

Abstract: The present disclosure discloses a control method and a control apparatus for speech interaction. The detailed implementation solution of the control method for the speech interaction includes: collecting an audio signal; detecting a wake-up word in the audio signal to obtain a wake-up word result; and playing a prompt tone and/or executing a speech instruction in the audio signal based on the wake-up word result.

Abstract: The embodiments of the present disclosure provides a smart service method, apparatus and device, the method includes: a first device plays response information corresponding to an awaking word after receiving the awaking word; when the first device determines that a second device in an awake state exists in a device group that the first device is in, the first device transmits operation information of the first device to the second device, and receives operation information of the second device transmitted by the second device; the first device determines, according to the operation information of the first device and the operation information of the second device a target device from the first device and the second device, where the target device is a device in the device group to provide a smart service. The service resources are economized.