Abstract: The present disclosure provides a method for screening a probiotic strain in promoting reproductive health by using a reproductive disorder model of Caenorhabditis elegans. Cyclophosphamide (CTX) drug intervention is applied to the C. elegans to destroy a normal reproductive system of the C. elegans, thereby obtaining the reproductive disorder model of the C. elegans. Strains capable of significantly improving a reproductive capacity of individuals with reproductive disorders are selected from 3,985 kinds of gene-knockout strains in a Keio gene-knockout library of Escherichia coli; and homologous gene knockout is conducted on a probiotic strain E. coli Nissle 1917 by gene editing, and a probiotic strain with a potential to promote the reproductive health is selected.

Abstract: The invention relates to the application of the ERH gene in the preparation of bladder cancer diagnosis and treatment products. The ERH gene is found to have a good correlation with bladder cancer. In addition, an RNA interference vector is constructed to perform cell function experiments to study the effect of ERH gene on the proliferation and clonality of bladder cancer cells. The present invention provides a research basis for clinical diagnosis and treatment of bladder cancer and has a good application prospect.

Abstract: The invention relates to the application of the ERH gene in the preparation of bladder cancer diagnosis and treatment products. The ERH gene is found to have a good correlation with bladder cancer. In addition, an RNA interference vector is constructed to perform cell function experiments to study the effect of ERH gene on the proliferation and clonality of bladder cancer cells. The present invention provides a research basis for clinical diagnosis and treatment of bladder cancer and has a good application prospect.

Abstract: The invention provides an experimental research method for targeted therapy of prostate cancer by nuclide 125I-labeled dual-regulation oncolytic adenovirus, comprising cloning and identification of prostate specific antigen PSA promoter; of adenoid construction and identification of hTERT/PSA double-regulated adenovirus vector RSOAds-hTERT/PSA; nuclide 125I-labeled hTERT/PSA dual-regulation proliferative oncolytic adenovirus construction 125I-RSOAds-hTERT/PSA nuclide-oncolytic virus marker; detection of transfection efficiency and tumor killing effect of 125I-RSOAds-hTERT/PSA on hormone-independent prostate cancer cells in vitro; 125I-RSOAds-hTERT/PSA targeted therapy for anti-tumor effect of prostate cancer and observation of tumor microenvironment changes.

Abstract: Provided herein are methods of applying the ERH gene in the preparation of bladder cancer diagnosis and treatment products. The ERH gene is found to have a good correlation with bladder cancer. In addition, an RNA interference vector is constructed to perform cell function experiments to study the effect of ERH gene on the proliferation and clonality of bladder cancer cells. The methods disclosed herein provide a research basis for clinical diagnosis and treatment of bladder cancer and has a good application prospect.