Abstract: The present invention relates, in general, to diagnostic kits for selectively detecting a prokaryotic microorganism and a eukaryotic microorganism in a sample wherein the cells of such microorganisms are lysed by combining the sample with a lysis solution and contacting the nucleic acid released from the microorganisms with selective nucleic acid probes through hybridization techniques. The present invention can be used for detecting microorganisms associated with vaginal disorders, e.g., Gardnerella vaginalis, Trichomonas vaginalis and Candida albicans. These kits may be used in a medical practitioner's private office or in a more structured clinical environment, such as a hospital, a commercial clinical microbiology laboratory or the like.

Abstract: The present invention relates, in general, to methods for selectively detecting a prokaryotic microorganism and a eukaryotic microorganism in a single complex biological sample wherein the cells of such microorganisms are lysed by combining the sample with a lysis solution and contacting the nucleic acid released from the microorganisms with selective nucleic acid probes through hybridization techniques. Further provided are methods which permit the detection of a Gram-positive bacterium with at least one other microorganism selected from the group consisting of yeast, protozoa, mycoplasma and Gram-negative bacteria in a single complex biological sample. In other aspects of the invention, the methods disclosed permit the detection of microorganisms associated with vaginal disorders.