Patents by Inventor Constantine Londos

Constantine Londos has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Cloning of perilipin proteins
    Patent number: 6074842
    Abstract: The present invention provides isolated nucleic acid sequences, i.e., polynucleotides, which encode a family of perilipin proteins. The present invention also provides isolated, substantially purified perilipin proteins which are useful as markers for differentiating true adipocytes from non-adipocyte cells which, as a result of pathophysiological conditions, assume adipocyte characteristics and become lipid-laden. The present invention further provides methods for producing a substantially purified perilipin protein and methods for detecting the presence of such perilipin proteins in a biological samples.
    Type: Grant
    Filed: December 16, 1996
    Date of Patent: June 13, 2000
    Assignee: The United States of America as represented by the Department of Health and Human Services
    Inventors: Constantine Londos, Andrew S. Greenberg, Alan R. Kimmel, John J. Egan
  • Cloning of perilipin proteins
    Patent number: 5585462
    Abstract: The present invention provides isolated nucleic acid sequences, i.e., polynucleotides, which encode a family of perilipin proteins. The present invention also provides isolated, substantially purified perilipin proteins which are useful as markers for differentiating true adipocytes from non-adipocyte cells which, as a result of pathophysiological conditions, assume adipocyte characteristics and become lipid-laden. The present invention further provides methods for producing a substantially purified perilipin protein and methods for detecting the presence of such perilipin proteins in a biological samples.
    Type: Grant
    Filed: October 4, 1993
    Date of Patent: December 17, 1996
    Assignee: United States of America
    Inventors: Constantine Londos, Andrew S. Greenberg, Alan R. Kimmel, John J. Egan
  • Assay of protein kinases with peptide substrates
    Patent number: 5141852
    Abstract: A method for assaying protein kinases that phosphorylate peptides such as Kemptide, such as cAMP-dependent protein kinase, or a glycogen synthase peptide, which is an excellent substrate for protein kinase C. Upon sequentially processing of reaction mixtures through tandem columns of cation and anion exchange resins improved separation of ATP from phosphorylated peptides is achieved such that radioactivity in background samples is nearly nil and the yield of phosphorylated peptides is high. This method is generally applicable to any protein kinase so long as the substrate peptide is appropriately structured such that the peptide retains a net positive charge when fully phosphorylated so that the peptide will adhere to the cation exchange resin and pass through the anion exchange resin. This method reduces labor, radioactivity, enzyme requirements, and costs of assaying protein kinases.
    Type: Grant
    Filed: December 12, 1988
    Date of Patent: August 25, 1992
    Assignee: The United States of America as represented by the Department of Health and Human Services
    Inventors: John J. Egan, Constantine Londos