Patents by Inventor Cordula Nemetz

Cordula Nemetz has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for improving the stability of linear DNA in cell-free in vitro transcription/translation systems
    Patent number: 7011958
    Abstract: The present invention concerns a method for improving the stability of linear short DNA towards exonucleases in cell-free in vitro transcription/translation systems using lysates containing exonucleases or in cellular systems containing exonucleases, wherein the stability of the linear short DNA is improved by adding unspecific linear DNA.
    Type: Grant
    Filed: April 17, 2002
    Date of Patent: March 14, 2006
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Manfred Watzele, Thomas Hoffmann, Cordula Nemetz, Dieter Heindl, Thomas Metzler, Wolfgang Mutter
  • Method for predicting the expression efficiency in cell-free expression systems
    Publication number: 20060024679
    Abstract: The invention relates to a method for the analysis and optimization of the expression efficiency in the preparation of a protein in expression systems and to a method for the preparation of proteins in such expression systems.
    Type: Application
    Filed: August 6, 2004
    Publication date: February 2, 2006
    Applicant: Biomax Informatics AG
    Inventors: Dieter Voges, Bernd Buchberger, Sabine Wizemann, Manfred Watzele, Cordula Nemetz
  • Method for protein expression starting from stabilized linear short DNA in cell-free in vitro transcription/translation systems with exonuclease-containing lysates or in a cellular system containing exonucleases
    Publication number: 20040259081
    Abstract: The present invention concerns a method for protein expression comprising the steps of transcribing stabilized linear short DNA in cell-free in vitro transcription/translation systems with exonuclease-containing lysates or in a cellular system containing exonucleases and subsequent translation, wherein the stability of the linear short DNA is improved by one or several of the following measures to protect the double-stranded DNA from exonucleases:
    Type: Application
    Filed: April 17, 2002
    Publication date: December 23, 2004
    Inventors: Manfred Watzele, Thomas Hoffmann, Cordula Nemetz, Dieter Heindl, Thomas Metzler, Wolfgang Mutter
  • Method for increasing the solubility, expression rate and the acitivity of proteins during recombinant production
    Publication number: 20040248238
    Abstract: The present invention concerns a method for producing a lysate containing helper proteins in which a strain which is suitable for obtaining in vitro translation lysates is transformed using a vector containing one or more genes coding for one or more helper proteins, wherein the helper proteins are expressed in this strain and the lysate containing helper proteins is obtained from these strains. The present invention also concerns a lysate containing helper proteins that can be obtained by the method according to the invention, blends of these lysates and the use of these lysates and blends in in vitro translation systems.
    Type: Application
    Filed: March 17, 2004
    Publication date: December 9, 2004
    Inventors: Manfred Watzele, Regina Schweizer, Cordula Nemetz, Robin Steigerwald, Thomas Emrich, Katrin Zaiss, Erhard Fernholz, Baerbel Walckhoff, Hans Joachim Schoenfeld, Birgit Offen
  • Method for producing linear DNA fragments for the in vitro expression of proteins
    Publication number: 20040110135
    Abstract: The present invention concerns a method for producing linear DNA fragments for the in vitro expression of proteins in which a linear DNA fragment which contains control elements necessary for expression and the protein-coding gene which has complementary regions to the two ends of the linear DNA fragment at both ends, are amplified together using a primer pair which binds upstream and downstream of the expression control regions on the linear DNA fragment, characterized in that the linear DNA fragment can be produced by linearizing an expression vector. Furthermore a method is disclosed which concerns the preparation of the protein-coding gene the ends of which have regions which overlap the linear DNA fragment.
    Type: Application
    Filed: December 6, 2002
    Publication date: June 10, 2004
    Inventors: Cordula Nemetz, Bernd Buchberger, Manfred Watzele, Wolfgang Mutter, Albert Roeder, Stephanie Wessner
  • Method for improving the stability of linear DNA in cell-free in vitro transcription / translation systems
    Publication number: 20030073201
    Abstract: The present invention concerns a method for improving the stability of linear short DNA towards exonucleases in cell-free in vitro transcription/translation systems using lysates containing exonucleases or in cellular systems containing exonucleases, wherein the stability of the linear short DNA is improved by adding unspecific linear DNA.
    Type: Application
    Filed: April 17, 2002
    Publication date: April 17, 2003
    Inventors: Manfred Watzele, Thomas Hoffmann, Cordula Nemetz, Dieter Heindl, Thomas Metzler, Wolfgang Mutter