Abstract: The disclosure relates to isolated microorganisms—including novel strains of the microorganisms—microbial ensembles, and compositions comprising the same. Furthermore, the disclosure teaches methods of utilizing the described microorganisms, microbial compositions, and compositions comprising the same, in methods for modulating the agricultural production of ruminants. In particular aspects, the disclosure provides methods of increasing feed efficiency, and methods of decreasing acidosis.

Abstract: Disclosed herein are polypeptides having nuclease activity. Some of the polypeptides having nuclease activity were generated by an improved gene site mutagenesis (“GSSM”) method or the tailored multi-site combinatorial assembly (“TMCA”) method. Also disclosed are compositions and kits comprising the polypeptides having nuclease activity, and methods for making and using these polypeptides, compositions and kits.

Abstract: The disclosure relates to isolated microorganisms—including novel strains of the microorganisms—microbial ensembles, and compositions comprising the same. Furthermore, the disclosure teaches methods of utilizing the described microorganisms, microbial compositions, and compositions comprising the same, in methods for modulating the agricultural production of ruminants. In particular aspects, the disclosure provides methods of increasing feed efficiency, and methods of decreasing acidosis.

Abstract: The present disclosure relates to methods of stabilization of microbial compositions comprising combining a population of pre-served microbial cells with at least one water activity scavenger (WAS) to a desired homogeneity level; and packaging and sealing the mixture of preserved microbial cells and the WAS. The present disclosure further relates to the stabilized microbial compositions and uses thereof.

Abstract: The present disclosure provides methods of improving microbe viability after preservation comprising subjecting a population of target microbial cells to one or more preservation challenges and preparing a product using the population of preserved viability-enhanced microbial cells produced from said methods. The present disclosure further provides products comprising preserved viability-enhanced microbial cells produced by the methods described herein.

Abstract: Disclosed herein are methods of identifying promoters that drive protein expression independently of methanol and are useful in driving protein expression in yeast.

Abstract: The disclosure relates to isolated microorganisms—including novel strains of the microorganisms—microbial ensembles, and compositions comprising the same. Furthermore, the disclosure teaches methods of utilizing the described microorganisms, microbial compositions, and compositions comprising the same, in methods for modulating the agricultural production of ruminants. In particular aspects, the disclosure provides methods of increasing feed efficiency, and methods of decreasing acidosis.

Abstract: Disclosed herein are polypeptides having nuclease activity. Some of the polypeptides having nuclease activity were generated by an improved gene site mutagenesis (“GSSM”) method or the tailored multi-site combinatorial assembly (“TMCA”) method. Also disclosed are compositions and kits comprising the polypeptides having nuclease activity, and methods for making and using these polypeptides, compositions and kits.

Abstract: The disclosure relates to isolated microorganisms—including novel strains of the microorganisms—microbial ensembles, and compositions comprising the same. Furthermore, the disclosure teaches methods of utilizing the described microorganisms, microbial compositions, and compositions comprising the same, in methods for modulating the agricultural production of ruminants. In particular aspects, the disclosure provides methods of increasing feed efficiency, and methods of decreasing acidosis.

Abstract: Systems and methods are provided for using a growth vessel to simulate algae growth and/or productivity in a reference environment, such as an open pond, a closed photobioreactor, or a hybrid system. Based on predicted algae sample trajectories in the reference environment, an illumination profile is developed. An algae sample in the growth vessel can then be exposed to the illumination profile under controlled conditions. Properties of algae in the reference environment can then be characterized based on the sample exposed to the illumination profile.

Abstract: Systems and methods are provided for using a growth vessel to simulate algae growth and/or productivity in a reference environment, such as an open pond, a closed photobioreactor, or a hybrid system. Based on predicted algae sample trajectories in the reference environment, an illumination profile is developed. An algae sample in the growth vessel can then be exposed to the illumination profile under controlled conditions. Properties of algae in the reference environment can then be characterized based on the sample exposed to the illumination profile.