Abstract: Disclosed herein are novel 3D microelectrode arrays (3D MEA) that include a substrate body (e.g. chip), microneedles, traces, and a well, wherein the 3D MEA provides for transfer of electrical signals on one side of the substrate body to the other side of the substrate body. Methods for using 3D MEAs to grow electrogenic cells and obtain electrophysiological signals are disclosed as well. Fabrication techniques for producing the 3D MEAs are also disclosed.

Abstract: An apparatus providing an embedded electrode array (EEA) capable of testing cell samples includes an embedded electrode array, an interface printed circuit board, a test controller for configuring the stimulating signals, and data channels in the interface printed circuit board for receiving and sampling responsive signals into data channels, and a data collector for receiving data sets for processing and storage. The embedded electrode array includes tissue culture wells each having electrode pads for containing a tissue cell sample signal connectors having a connector pins for receiving a stimulating signal for each of tissue culture wells and for generating a plurality of responsive signals from each of the tissue cell samples, and circuit runs embedded within an EEA-printed circuit board, for connecting the one of the connector pins to of the electrode pads.

Abstract: Disclosed herein are novel 3D microelectrode arrays (3D MEA) that include a substrate body (e.g. chip), microneedles, traces, and a well, wherein the 3D MEA provides for transfer of electrical signals on one side of the substrate body to the other side of the substrate body. Methods for using 3D MEAs to grow electrogenic cells and obtain electrophysiological signals are disclosed as well. Fabrication techniques for producing the 3D MEAs are also disclosed.