Abstract: The present invention relates to the treatment of infections due to antibiotic-resistant bacteria. Antimicrobial resistance (AMR) has been observed at dangerously high levels worldwide and alternative strategies are urgently needed. Antisense therapy has been identified as potential therapeutic tool for tackling AMR. However, in the context of AMR, since the antisense oligonucleotides have to reach the target mRNA to be efficient, the cellular uptake inside prokaryotic cells is a critical issue. The inventors demonstrated that antisense oligonucleotide sequences, in particular targeting the blaCTX/M15 gene, featuring a lipid moiety conjugated to the ASO extremity show a particularly efficient intracellular penetration in prokaryotic cells and that these lipid-modified antisense oligonucleotides can show a further improved enzymatic stability with phosphorothioate chemistry (PTO).