Abstract: A separation apparatus is disclosed, comprising: a layerizer arranged to bring a group of particles in a layer on a transport surface with a constant spatial relation of the particles relative to each other in the layer; an identifier arranged to identify particles in the group of particles that have a specific property; a separator arranged to separate the particles in the group based on a difference in affinity between the particles and the separator; an affinity modifier arranged to modify said affinity for identified particles relative to non-identified particles in the group. The layerizer comprises a recirculating transport surface on which the particles of the layer are carried. The transport surface is arranged to move along a transport trajectory as a rigid plane. Further, a method for separation of particles from a group of particles is disclosed.